دورية أكاديمية
Graft Protective and Intercellular Immunomodulatory Effects by Adoptive Transfer of an Agonistic Anti-BTLA mAb (3C10) Induced CD4 + CD25 + Regulatory T Cells in Murine Cardiac Allograft Transplant Model.
| العنوان: | Graft Protective and Intercellular Immunomodulatory Effects by Adoptive Transfer of an Agonistic Anti-BTLA mAb (3C10) Induced CD4 + CD25 + Regulatory T Cells in Murine Cardiac Allograft Transplant Model. |
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| المؤلفون: | Masaoka H; Department of Cardiovascular Surgery, Teikyo University, Tokyo, Japan., Yamamoto Y; Department of Cardiovascular Surgery, Teikyo University, Tokyo, Japan., Uchiyama M; Department of Cardiovascular Surgery, Teikyo University, Tokyo, Japan. Electronic address: mautiya@yahoo.co.jp., Iguchi K; Department of Cardiovascular Surgery, Teikyo University, Tokyo, Japan., Nakamura M; Department of Cardiovascular Surgery, Teikyo University, Tokyo, Japan., Yagita H; Department of Immunology, Juntendo University, Tokyo, Japan., Imazuru T; Department of Cardiovascular Surgery, Teikyo University, Tokyo, Japan., Shimokawa T; Department of Cardiovascular Surgery, Teikyo University, Tokyo, Japan. |
| المصدر: | Transplantation proceedings [Transplant Proc] 2024 Apr; Vol. 56 (3), pp. 692-700. Date of Electronic Publication: 2024 Feb 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Inc Country of Publication: United States NLM ID: 0243532 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2623 (Electronic) Linking ISSN: 00411345 NLM ISO Abbreviation: Transplant Proc Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, N.Y. : Elsevier Science Inc. Original Publication: New York Stratton. |
| مواضيع طبية MeSH: | Heart Transplantation* , T-Lymphocytes, Regulatory*/immunology , Mice, Inbred C57BL* , Adoptive Transfer* , Mice, Inbred CBA* , Graft Survival*/drug effects, Animals ; Mice ; Interleukin-2 Receptor alpha Subunit/immunology ; Antibodies, Monoclonal/pharmacology ; Male ; Receptors, Immunologic/metabolism ; Allografts ; Graft Rejection/immunology ; Graft Rejection/prevention & control ; Mice, Inbred BALB C |
| مستخلص: | Background: We demonstrated that an agonistic anti-B and T lymphocyte attenuator antibody (3C10) prolonged cardiac survival by inducing regulatory T cells (Treg). However, the mechanisms of immune tolerance in the recipients remained unclear. In this study, we investigated the graft-protective and intercellular immunomodulatory effects of adoptive transfer (AT) of 3C10-induced Tregs in a murine cardiac allograft transplant model. Methods: Thirty days after transplantation of a C57BL/6 heart into the primary 3C10-treated CBA recipients, splenic CD4 + CD25 + cells from these recipients (3C10/AT group) or naïve CBA mice (no-treatment group) were adoptively transferred into secondary CBA recipients with a C57BL/6 heart. To confirm the requirement for 3C10-induced Tregs, we administered an anti-interleukin-2 receptor alpha antibody (PC-61) to secondary CBA recipients. Additionally, histologic and fluorescent staining, cell proliferation analysis, flow cytometry, and donor-specific antibody (DSA) measurements were performed. Results: 3C10/AT-treated CBA recipients resulted in significantly prolonged allograft survival (median survival time [MST], >50 days). Allografts displayed prolonged function with preservation of vessel structure by maintaining high numbers of splenic CD4 + CD25 + Foxp3 + Treg and intramyocardial CD4 + Foxp3 + cells. DSA levels were suppressed in 3C10/AT-treated CBA recipients. Moreover, PC-61 administration resulted in a shorter MSTs of cardiac allograft survivals, a detrimental increase in DSA production, and enhanced expression of programmed cell death (PD)-1. Conclusion: AT of 3C10-induced Tregs may be a promising graft-protective strategy to prolong allograft survival and suppress DSA production, driven by the promotion of splenic and graft-infiltrating Tregs and collaboration with PD-1 + T cells and Treg. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Inc. All rights reserved.) |
| المشرفين على المادة: | 0 (Interleukin-2 Receptor alpha Subunit) 0 (Antibodies, Monoclonal) 0 (Receptors, Immunologic) 0 (PC61 monoclonal antibody) |
| تواريخ الأحداث: | Date Created: 20240215 Date Completed: 20240426 Latest Revision: 20240426 |
| رمز التحديث: | 20240427 |
| DOI: | 10.1016/j.transproceed.2024.01.015 |
| PMID: | 38360464 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1873-2623 |
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| DOI: | 10.1016/j.transproceed.2024.01.015 |