دورية أكاديمية
Polygonum cillinerve polysaccharide inhibits transmissible gastroenteritis virus by regulating microRNA-181.
| العنوان: | Polygonum cillinerve polysaccharide inhibits transmissible gastroenteritis virus by regulating microRNA-181. |
|---|---|
| المؤلفون: | Duan X; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China; Agricultural Management Department, Sichuan Xuanhan Vocational Secondary School, Xuanhan 636350, PR China., Li H; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China., Tan X; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China., Liu N; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China., Wang X; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China., Zhang W; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China., Liu Y; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China., Ma W; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China., Wu Y; Nanjing Agricultural University, No 1 Weigang, Nanjing 210095, PR China. Electronic address: wuyi2001cn@163.com., Ma L; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China. Electronic address: linma@nwafu.edu.cn., Fan Y; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China. Electronic address: ypfan@nwsuaf.edu.cn. |
| المصدر: | Veterinary journal (London, England : 1997) [Vet J] 2024 Apr; Vol. 304, pp. 106083. Date of Electronic Publication: 2024 Feb 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Balliere Tindall Country of Publication: England NLM ID: 9706281 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2971 (Electronic) Linking ISSN: 10900233 NLM ISO Abbreviation: Vet J Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Balliere Tindall Original Publication: London : Ballière Tindall, c1997- |
| مواضيع طبية MeSH: | Transmissible gastroenteritis virus*/genetics , Polygonum*/genetics , MicroRNAs*/genetics, Animals ; Swine ; RNA, Guide, CRISPR-Cas Systems ; Signal Transduction |
| مستخلص: | Transmissible gastroenteritis virus (TGEV) is an important pathogen capable of altering the expression profile of cellular miRNA. In this study, the potential of Polygonum cillinerve polysaccharide (PCP) to treat TGEV-infected piglets was evaluated through in vivo experiments. High-throughput sequencing technology was employed to identify 9 up-regulated and 17 down-regulated miRNAs during PCP-mediated inhibition of TGEV infection in PK15 cells. Additionally, miR-181 was found to be associated with target genes of key proteins in the apoptosis pathway. PK15 cells were treated with various concentrations of PCP following transfection with miR-181 mimic or inhibitor. Real-time PCR assessed the impact on TGEV replication, while electron microscopy (TEM) and Hoechst fluorescence staining evaluated cellular functionality. Western blot analysis was utilized to assess the expression of key signaling factors-cytochrome C (cyt C), caspase 9, and P53-in the apoptotic signaling pathway. The results showed that compared with the control group, 250 μg/mL PCP significantly inhibited TGEV gRNA replication and gene N expression (P < 0.01). Microscopic examination revealed uniform cell morphology and fewer floating cells in PCP-treated groups (250 and 125 μg/mL). TEM analysis showed no typical virus structure in the 250 μg/mL PCP group, and apoptosis staining indicated a significant reduction in apoptotic cells at this concentration. Furthermore, PCP may inhibit TGEV-induced apoptosis via the Caspase-dependent mitochondrial pathway following miR-181 transfection. These findings provide a theoretical basis for further exploration into the mechanism of PCP's anti-TGEV properties. Competing Interests: Conflict of Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Apoptosis; MiR-181; Polygonum cillinerve polysaccharide; Transmissible gastroenteritis virus; Viral replication |
| المشرفين على المادة: | 0 (RNA, Guide, CRISPR-Cas Systems) 0 (MicroRNAs) |
| تواريخ الأحداث: | Date Created: 20240216 Date Completed: 20240416 Latest Revision: 20240501 |
| رمز التحديث: | 20240501 |
| DOI: | 10.1016/j.tvjl.2024.106083 |
| PMID: | 38365083 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1532-2971 |
|---|---|
| DOI: | 10.1016/j.tvjl.2024.106083 |