دورية أكاديمية
tRF-29-79 regulates lung adenocarcinoma progression through mediating glutamine transporter SLC1A5.
| العنوان: | tRF-29-79 regulates lung adenocarcinoma progression through mediating glutamine transporter SLC1A5. |
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| المؤلفون: | Shi Y; Department of Thoracic Surgery, Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, 42 Baiziting Road, Xuanwu District, Nanjing 210009, China.; Jiangsu Key Laboratory of Molecular and Translational Cancer Research, 42 Baiziting Road, Xuanwu District, Nanjing 210009, China.; The Fourth Clinical College of Nanjing Medical University, Nanjing, China., Pan Z; Department of Thoracic Surgery, Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, 42 Baiziting Road, Xuanwu District, Nanjing 210009, China.; Jiangsu Key Laboratory of Molecular and Translational Cancer Research, 42 Baiziting Road, Xuanwu District, Nanjing 210009, China.; The Fourth Clinical College of Nanjing Medical University, Nanjing, China., Feng Y; Department of Thoracic Surgery, Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, 42 Baiziting Road, Xuanwu District, Nanjing 210009, China.; Jiangsu Key Laboratory of Molecular and Translational Cancer Research, 42 Baiziting Road, Xuanwu District, Nanjing 210009, China.; The Fourth Clinical College of Nanjing Medical University, Nanjing, China., Zhou Q; The First Clinical College of Nanjing Medical University, Nanjing, China., Wang Q; Department of Thoracic Surgery, Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, 42 Baiziting Road, Xuanwu District, Nanjing 210009, China.; Jiangsu Key Laboratory of Molecular and Translational Cancer Research, 42 Baiziting Road, Xuanwu District, Nanjing 210009, China.; The Fourth Clinical College of Nanjing Medical University, Nanjing, China., Wang H; Department of Thoracic Surgery, Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, 42 Baiziting Road, Xuanwu District, Nanjing 210009, China.; Jiangsu Key Laboratory of Molecular and Translational Cancer Research, 42 Baiziting Road, Xuanwu District, Nanjing 210009, China.; The Fourth Clinical College of Nanjing Medical University, Nanjing, China., Dong G; Department of Thoracic Surgery, Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, 42 Baiziting Road, Xuanwu District, Nanjing 210009, China.; Jiangsu Key Laboratory of Molecular and Translational Cancer Research, 42 Baiziting Road, Xuanwu District, Nanjing 210009, China., Xia W; Department of Thoracic Surgery, Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, 42 Baiziting Road, Xuanwu District, Nanjing 210009, China.; Jiangsu Key Laboratory of Molecular and Translational Cancer Research, 42 Baiziting Road, Xuanwu District, Nanjing 210009, China.; The Fourth Clinical College of Nanjing Medical University, Nanjing, China., Jiang F; Department of Thoracic Surgery, Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, 42 Baiziting Road, Xuanwu District, Nanjing 210009, China.; Jiangsu Key Laboratory of Molecular and Translational Cancer Research, 42 Baiziting Road, Xuanwu District, Nanjing 210009, China.; The Fourth Clinical College of Nanjing Medical University, Nanjing, China. |
| المصدر: | Carcinogenesis [Carcinogenesis] 2024 Jun 10; Vol. 45 (6), pp. 409-423. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Irl Press At Oxford University Press Country of Publication: England NLM ID: 8008055 Publication Model: Print Cited Medium: Internet ISSN: 1460-2180 (Electronic) Linking ISSN: 01433334 NLM ISO Abbreviation: Carcinogenesis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Irl Press At Oxford University Press Original Publication: [New York, IRL Press] |
| مواضيع طبية MeSH: | Adenocarcinoma of Lung*/pathology , Adenocarcinoma of Lung*/genetics , Adenocarcinoma of Lung*/metabolism , Polypyrimidine Tract-Binding Protein*/metabolism , Polypyrimidine Tract-Binding Protein*/genetics , Amino Acid Transport System ASC*/metabolism , Amino Acid Transport System ASC*/genetics , Lung Neoplasms*/pathology , Lung Neoplasms*/genetics , Lung Neoplasms*/metabolism , Cell Proliferation* , Heterogeneous-Nuclear Ribonucleoproteins*/metabolism , Heterogeneous-Nuclear Ribonucleoproteins*/genetics , Gene Expression Regulation, Neoplastic* , Disease Progression*, Humans ; Mice ; Animals ; Minor Histocompatibility Antigens/genetics ; Minor Histocompatibility Antigens/metabolism ; Cell Movement ; Prognosis ; Cell Line, Tumor ; Alternative Splicing ; Female ; Glutamine/metabolism ; Male |
| مستخلص: | In recent decades, considerable evidence has emerged indicating the involvement of tRNA-derived fragments (tRFs) in cancer progression through various mechanisms. However, the biological effects and mechanisms of tRFs in lung adenocarcinoma (LUAD) remain unclear. In this study, we screen out tRF-29-79, a 5'-tRF derived from tRNAGlyGCC, through profiling the tRF expressions in three pairs of LUAD tissues. We show that tRF-29-79 is downregulated in LUAD and downregulation of tRF-29-79 is associated with poorer prognosis. In vivo and in vitro assay reveal that tRF-29-79 inhibits proliferation, migration and invasion of LUAD cells. Mechanistically, we discovered that tRF-29-79 interacts with the RNA-binding protein PTBP1 and facilitates the transportation of PTBP1 from nucleus to cytoplasm, which regulates alternative splicing in the 3' untranslated region (UTR) of SLC1A5 pre-mRNA. Given that SLC1A5 is a core transporter of glutamine, we proved that tRF-29-79 mediate glutamine metabolism of LUAD through affecting the stability of SLC1A5 mRNA, thus exerts its anticancer function. In summary, our findings uncover the novel mechanism that tRF-29-79 participates in glutamine metabolism through interacting with PTBP1 and regulating alternative splicing in the 3' UTR of SLC1A5 pre-mRNA. (© The Author(s) 2024. Published by Oxford University Press. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.) |
| معلومات مُعتمدة: | 82003106 National Natural Science Foundation of China; ZDXYS202203 Jiangsu Provincial Medical Key Laboratory; CXZX202224 Jiangsu Provincial Medical Innovation Center |
| المشرفين على المادة: | 139076-35-0 (Polypyrimidine Tract-Binding Protein) 0 (Amino Acid Transport System ASC) 0 (SLC1A5 protein, human) 0 (PTBP1 protein, human) 0 (Heterogeneous-Nuclear Ribonucleoproteins) 0 (Minor Histocompatibility Antigens) 0RH81L854J (Glutamine) |
| تواريخ الأحداث: | Date Created: 20240217 Date Completed: 20240610 Latest Revision: 20240620 |
| رمز التحديث: | 20240620 |
| DOI: | 10.1093/carcin/bgae010 |
| PMID: | 38366384 |
| قاعدة البيانات: | MEDLINE |
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