دورية أكاديمية
أعرض في EDS

The possible regulatory role of miR-514 and miR-642b in cumulus cells on the oocyte maturation in patients with polycystic ovary syndrome.

التفاصيل البيبلوغرافية
العنوان: The possible regulatory role of miR-514 and miR-642b in cumulus cells on the oocyte maturation in patients with polycystic ovary syndrome.
المؤلفون: Shafienia H; Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran., Hoseini SM; Biotechnology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran., Khalili MA; Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran., Heydari L; Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran., Sheikhha MH; Biotechnology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran. Electronic address: sheikhha@yahoo.com., Montazeri F; Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran. Electronic address: f.montazeri@ssu.ac.ir.
المصدر: Reproductive biology [Reprod Biol] 2024 Jun; Vol. 24 (2), pp. 100863. Date of Electronic Publication: 2024 Feb 16.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Urban & Partner Country of Publication: Poland NLM ID: 101160559 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2300-732X (Electronic) Linking ISSN: 1642431X NLM ISO Abbreviation: Reprod Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: <2013- > : Olsztyn, Poland : Elsevier Urban & Partner
Original Publication: Olsztyn, Poland : Polish Society for Biology of Reproduction, c2001-
مواضيع طبية MeSH: Polycystic Ovary Syndrome*/genetics , Polycystic Ovary Syndrome*/metabolism , MicroRNAs*/metabolism , MicroRNAs*/genetics , Cumulus Cells*/metabolism , Oocytes*/metabolism, Humans ; Female ; Adult ; ErbB Receptors/metabolism ; ErbB Receptors/genetics ; Receptors, Prostaglandin E, EP2 Subtype/metabolism ; Receptors, Prostaglandin E, EP2 Subtype/genetics ; Oogenesis/genetics
مستخلص: Polycystic ovary syndrome is a common endocrine disorder in reproductive-age women. Accordingly, abnormal microenvironment may negatively influence oocyte developmental competence as a result of the altered expression profile of cumulus cells (CCs), mainly the key players of oocyte maturation, such as epidermal growth factor receptor (EGFR) and prostaglandin E receptor-2 (PTGER2). This study aimed to examine the expression levels of miR-514, miR-642b, and their candidate target genes (EGFR and PTGER2, respectively) in CCs of immature and mature oocytes in patients with PCOS. A total of 40 oocytes at germinal vesicle (GV) and 40 oocytes at metaphase II (MII) stages were retrieved from 30 PCOS women. Quantitative real-time PCR was performed to analyze the expression level of miR-514, miR-642b, EGFR, and PTGER2 in cumulus cells (CCS) of each oocyte. The expression level of miRNAs and their candidate target genes were compared between CCs of GV and MII oocytes. Our study suggests an inverse relationship exists between the expression levels of miR-514 and EGFR, and miR-642b and PTGER2. Furthermore, we observed that CCs of GV oocytes had higher levels of EGFR and PTGER2 mRNA and lower levels of miR-514 and miR-642b expression compared to those of MII oocytes. The present study demonstrated that miR-514 and miR-642b can regulate oocyte development by targeting EGFR and PTGER2, respectively. Therefore, examination of these miRNAs in CCs could be promising parameters to predict oocyte competence in PCOS patients.
Competing Interests: Declaration of Competing Interest The authors declare that there is no conflict of interest regarding this article's publication and financial issues.
(Copyright © 2024 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Cumulus cells; MiR-514; MiR-642b; Oocyte competence; Polycystic ovary syndrome
المشرفين على المادة: 0 (MicroRNAs)
EC 2.7.10.1 (ErbB Receptors)
0 (Receptors, Prostaglandin E, EP2 Subtype)
0 (PTGER2 protein, human)
EC 2.7.10.1 (EGFR protein, human)
تواريخ الأحداث: Date Created: 20240217 Date Completed: 20240606 Latest Revision: 20240617
رمز التحديث: 20240617
DOI: 10.1016/j.repbio.2024.100863
PMID: 38367330
قاعدة البيانات: MEDLINE
الوصف
تدمد:2300-732X
DOI:10.1016/j.repbio.2024.100863