دورية أكاديمية
أعرض في EDS

Effects of β-mannanase supplementation on productive performance, inflammation, energy metabolism, and cecum microbiota composition of laying hens fed with reduced-energy diets.

التفاصيل البيبلوغرافية
العنوان: Effects of β-mannanase supplementation on productive performance, inflammation, energy metabolism, and cecum microbiota composition of laying hens fed with reduced-energy diets.
المؤلفون: Liu H; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China., Liu W; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China., Ai M; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China., Hao X; Qingdao Animal Husbandry Workstation (Qingdao Research Institute of Husbandry and Veterinary), Qingdao 266100, China., Zhang Q; Qingdao Animal Husbandry Workstation (Qingdao Research Institute of Husbandry and Veterinary), Qingdao 266100, China., Ren J; Qingdao Animal Husbandry Workstation (Qingdao Research Institute of Husbandry and Veterinary), Qingdao 266100, China., Zhang K; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China. Electronic address: zhangkai@qau.edu.cn.
المصدر: Poultry science [Poult Sci] 2024 Apr; Vol. 103 (4), pp. 103521. Date of Electronic Publication: 2024 Feb 01.
نوع المنشور: Randomized Controlled Trial, Veterinary; Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: England NLM ID: 0401150 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-3171 (Electronic) Linking ISSN: 00325791 NLM ISO Abbreviation: Poult Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: 2020- : [Cambridge, UK] : Elsevier
Original Publication: Champaign Il : Poultry Science Association
مواضيع طبية MeSH: beta-Mannosidase* , Microbiota*, Animals ; Female ; Chickens/physiology ; Interleukin-6 ; NF-kappa B ; Diet/veterinary ; Cecum ; Energy Metabolism ; Animal Feed/analysis ; Dietary Supplements/analysis ; Inflammation/veterinary ; RNA, Messenger
مستخلص: The objective of this study is to investigate the beneficial effects and underlying mechanism of dietary β-mannanase supplementation on the productive performance of laying hens fed with metabolic energy (ME)-reduced diets. A total of 448 Hy-Line gray laying hens were randomly assigned to seven groups. Each group had 8 replicates with 8 hens. The groups included a control diet (CON) with a ME of 2750 kcal/Kg, diets reduced by 100 kcal/Kg or 200 kcal/Kg ME (ME _100 or ME _200 ), and diets with 0.15 g/Kg or 0.2 g/Kg β-mannanase (ME _100 +β-M _0.15 , ME _100 +β-M _0.2 , ME _200 +β-M _0.15 , and ME _200 +β-M _0.2 ). The productive performance, egg quality, intestinal morphology, inflammatory response, mRNA expression related to the Nuclear factor kappa B (NF-κB) and AMPK pathway, and cecum microbiome were evaluated in this study. ME-reduced diets negatively impacted the productive performance of laying hens. However, supplementation with β-mannanase improved FCR, decreased ADFI, and restored average egg weight to the level of the CON group. ME-reduced diets increased the levels of interleukin-1β (IL-1β) and IL-6 while decreasing the levels of IL-4 and IL-10 in the jejunum of laying hens. However, dietary β-mannanase supplementation improved jejunum morphology, reduced pro-inflammatory cytokine concentrations, and increased levels of anti-inflammatory factors in laying hens fed with ME-reduced diets. The mRNA levels of IL-6, IFN-γ, TLR4, MyD88, and NF-κB in the jejunum of ME-reduced diets were significantly higher than that in CON, dietary β-mannanase supplementation decreased these genes expression in laying hens fed with ME-reduced diets. Moreover, dietary β-mannanase supplementation also decreased the mRNA levels of AMPKα and AMPKγ, and increased the abundance of mTOR in the jejunum of laying hens fed with ME-reduced diets. Cecum microbiota analysis revealed that dietary β-mannanase increased the abundance of various beneficial bacteria (e.g., g_Pseudoflavonifractor, g_Butyricicoccus, and f_Lactobacillaceae) in laying hens fed with ME-reduced diets. In conclusion, dietary β-mannanase supplementation could improve the productive performance of laying hens fed with a ME-reduced diet by improving intestinal morphology, alleviating intestinal inflammation, changing energy metabolism-related signaling pathways, and increasing cecum-beneficial microbiota.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: cecum microbiota; inflammatory response; laying hens; low-energy diet; β-Mannanase
المشرفين على المادة: EC 3.2.1.25 (beta-Mannosidase)
0 (Interleukin-6)
0 (NF-kappa B)
0 (RNA, Messenger)
تواريخ الأحداث: Date Created: 20240217 Date Completed: 20240322 Latest Revision: 20240322
رمز التحديث: 20240322
مُعرف محوري في PubMed: PMC10882124
DOI: 10.1016/j.psj.2024.103521
PMID: 38367470
قاعدة البيانات: MEDLINE
الوصف
تدمد:1525-3171
DOI:10.1016/j.psj.2024.103521