دورية أكاديمية
Evaluating the TaqMan Jr a -Genotyping Method for Rapidly Predicting the Presence of Anti-Jr a Antibodies.
| العنوان: | Evaluating the TaqMan Jr a -Genotyping Method for Rapidly Predicting the Presence of Anti-Jr a Antibodies. |
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| المؤلفون: | Koo YK; Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea., Kwon SS; Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea., Suh EJ; Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea., Kim NH; Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea., Kim HK; Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea., Cho YK; Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea., Choi SJ; Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea., Kim S; Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea., Lee KA; Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea. |
| المصدر: | Annals of laboratory medicine [Ann Lab Med] 2024 Sep 01; Vol. 44 (5), pp. 418-425. Date of Electronic Publication: 2024 Feb 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Korean Society for Laboratory Medicine Country of Publication: Korea (South) NLM ID: 101571172 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2234-3814 (Electronic) Linking ISSN: 22343806 NLM ISO Abbreviation: Ann Lab Med Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Seoul : Korean Society for Laboratory Medicine |
| مواضيع طبية MeSH: | Polymorphism, Single Nucleotide* , Blood Grouping and Crossmatching*/methods, Humans ; Genotype ; Genotyping Techniques/methods ; Isoantibodies/blood ; Erythrocytes/immunology ; Blood Group Antigens/genetics ; Blood Group Antigens/immunology ; Sequence Analysis, DNA |
| مستخلص: | Background: The Jr a antigen is a high-prevalence red blood cell (RBC) antigen. Reports on cases of fatal hemolytic disease of the fetus and newborn and acute hemolytic transfusion reactions suggest that antibodies against Jr a (anti-Jr a ) have potential clinical significance. Identifying anti-Jr a is challenging owing to a lack of commercially available antisera. We developed an alternative approach to rapidly predict the presence of anti-Jr a using the TaqMan single-nucleotide polymorphism (SNP)-genotyping method. Methods: Residual peripheral blood samples from 10 patients suspected of having the anti-Jr a were collected. Two samples with confirmed Jr(a-) RBCs and anti-Jr a were used to validate the TaqMan genotyping assay by comparing the genotyping results with direct sequencing. The accuracy of the assay in predicting the presence of anti-Jr a was verified through crossmatching with in-house Jr(a-) O+ RBCs. Results: The TaqMan-genotyping method was validated with two Jr(a-) RBC- and anti-Jr a -confirmed samples that showed concordant Jr a genotyping and direct sequencing results. Jr a genotyping for the remaining samples and crossmatching the serum samples with inhouse Jr(a-) O+ RBCs showed consistent results. Conclusions: We validated a rapid, simple, accurate, and cost-effective method for predicting the presence of anti-Jr a using a TaqMan-based SNP-genotyping assay. Implementing this method in routine practice in clinical laboratories will assist in solving difficult problems regarding alloantibodies to high-prevalence RBC antigens and ultimately aid in providing safe and timely transfusions and proper patient care. |
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| فهرسة مساهمة: | Keywords: Anti-Jra; Antibody identification; Genotyping; High-prevalence antigen; Jr(a–); Single-nucleotide polymorphism; TaqMan assay |
| المشرفين على المادة: | 0 (Isoantibodies) 0 (Blood Group Antigens) |
| تواريخ الأحداث: | Date Created: 20240219 Date Completed: 20240612 Latest Revision: 20240615 |
| رمز التحديث: | 20240615 |
| مُعرف محوري في PubMed: | PMC11169768 |
| DOI: | 10.3343/alm.2023.0325 |
| PMID: | 38373791 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2234-3814 |
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| DOI: | 10.3343/alm.2023.0325 |