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Integrated full-length transcriptome and metabolome analysis reveals the defence response of melon to gummy stem blight.

التفاصيل البيبلوغرافية
العنوان: Integrated full-length transcriptome and metabolome analysis reveals the defence response of melon to gummy stem blight.
المؤلفون: Wang H; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China., Wei X; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China., Mo C; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China., Wei M; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China., Li Y; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China., Fan Y; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China., Gu X; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China., Zhang X; Hami-Melon Research Center, Xinjiang Academy of Agricultural Sciences, Urumqi, China., Zhang Y; Hami-Melon Research Center, Xinjiang Academy of Agricultural Sciences, Urumqi, China., Kong Q; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China.
المصدر: Plant, cell & environment [Plant Cell Environ] 2024 Jun; Vol. 47 (6), pp. 1997-2010. Date of Electronic Publication: 2024 Feb 21.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: John Wiley & Sons Ltd Country of Publication: United States NLM ID: 9309004 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-3040 (Electronic) Linking ISSN: 01407791 NLM ISO Abbreviation: Plant Cell Environ Subsets: MEDLINE
أسماء مطبوعة: Publication: Hoboken, NJ : John Wiley & Sons Ltd.
Original Publication: Oxford, UK : Blackwell Scientific Publications
مواضيع طبية MeSH: Plant Diseases*/microbiology , Plant Diseases*/genetics , Plant Diseases*/immunology , Metabolome* , Transcriptome* , Disease Resistance*/genetics , Cucurbitaceae*/microbiology , Cucurbitaceae*/genetics , Cucurbitaceae*/metabolism , Gene Expression Regulation, Plant*, Gene Expression Profiling
مستخلص: Gummy stem blight (GSB), a widespread disease causing great loss to cucurbit production, has become a major threat to melon cultivation. However, the melon-GSB interaction remains largely unknown. Here, full-length transcriptome and widely targeted metabolome were used to investigate the defence responses of resistant (PI511089) and susceptible (Payzawat) melon accessions to GSB pathogen infection at 24 h. The biosynthesis of secondary metabolites and MAPK signalling pathway were specifically enriched for differentially expressed genes in PI511890, while carbohydrate metabolism and amino acid metabolism were specifically enriched in Payzawat. More than 1000 novel genes were identified and MAPK signalling pathway was specifically enriched for them in PI511890. There were 11 793 alternative splicing events involving in the defence response to GSB. Totally, 910 metabolites were identified in Payzawat and PI511890, and flavonoids were the dominant metabolites. Integrated full-length transcriptome and metabolome analysis showed eriodictyol and oxalic acid were the potential marker metabolites for GSB resistance in melon. Moreover, posttranscription regulation was widely involved in the defence response of melon to GSB pathogen infection. These results not only improve our understanding on the interaction between melon and GSB, but also facilitate the genetic improvement of melon with GSB resistance.
(© 2024 John Wiley & Sons Ltd.)
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معلومات مُعتمدة: 2021BBA101 Key R&D project of Hubei Province; 2662020YLPY024 Fundamental Research Funds for the Central Universities; 2022A03004-5 Major Project of Sci & Tech of Xinjiang; CARS-25-G2 China Agriculture Research System
فهرسة مساهمة: Keywords: alternative splicing; differentially accumulated metabolites; differentially expressed genes; novel genes; plant‐pathogen interaction
تواريخ الأحداث: Date Created: 20240221 Date Completed: 20240429 Latest Revision: 20240429
رمز التحديث: 20240429
DOI: 10.1111/pce.14865
PMID: 38379450
قاعدة البيانات: MEDLINE
الوصف
تدمد:1365-3040
DOI:10.1111/pce.14865