دورية أكاديمية
A novel perspective on eugenol as a natural anti-quorum sensing molecule against Serratia sp.
| العنوان: | A novel perspective on eugenol as a natural anti-quorum sensing molecule against Serratia sp. |
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| المؤلفون: | Frikha F; Laboratory of Molecular and Cellular Screening Processes, Centre of Biotechnology of Sfax, University of Sfax, P O Box 1177, Sidi Mansour Road, 3018, Sfax, Tunisia. Electronic address: fakher.frikha@fss.usf.tn., Jardak M; Laboratory of Molecular and Cellular Screening Processes, Centre of Biotechnology of Sfax, University of Sfax, P O Box 1177, Sidi Mansour Road, 3018, Sfax, Tunisia., Aifa S; Laboratory of Molecular and Cellular Screening Processes, Centre of Biotechnology of Sfax, University of Sfax, P O Box 1177, Sidi Mansour Road, 3018, Sfax, Tunisia., Mnif S; Laboratory of Molecular and Cellular Screening Processes, Centre of Biotechnology of Sfax, University of Sfax, P O Box 1177, Sidi Mansour Road, 3018, Sfax, Tunisia. |
| المصدر: | Microbial pathogenesis [Microb Pathog] 2024 Apr; Vol. 189, pp. 106576. Date of Electronic Publication: 2024 Feb 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 8606191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1208 (Electronic) Linking ISSN: 08824010 NLM ISO Abbreviation: Microb Pathog Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; Orlando : Academic Press, c1986- |
| مواضيع طبية MeSH: | Quorum Sensing* , Serratia*, Biofilms ; Eugenol/pharmacology ; Serratia marcescens ; Virulence Factors/metabolism ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/metabolism |
| مستخلص: | Serratia marcescens is commonly noted to be an opportunistic pathogen and is often associated with nosocomial infections. In addition to its high antibiotic resistance, it exhibits a wide range of virulence factors that confer pathogenicity. Targeting quorum sensing (QS) presents a potential therapeutic strategy for treating bacterial infections caused by S. marcescens, as it regulates the expression of various virulence factors. Inhibiting QS can effectively neutralize S. marcescens' bacterial virulence without exerting stress on bacterial growth, facilitating bacterial eradication by the immune system. In this study, the antibacterial and anti-virulence properties of eugenol against Serratia sp. were investigated. Eugenol exhibited inhibitory effects on the growth of Serratia, with a minimal inhibitory concentration (MIC) value of 16.15 mM. At sub-inhibitory concentrations, eugenol also demonstrated antiadhesive and eradication activities by inhibiting biofilm formation. Furthermore, it reduced prodigiosin production and completely inhibited protease production. Additionally, eugenol effectively decreased swimming and swarming motilities in Serratia sp. This study demonstrated through molecular modeling, docking and molecular dynamic that eugenol inhibited biofilm formation and virulence factor production in Serratia by binding to the SmaR receptor and blocking the formation of the HSL-SmaR complex. The binding of eugenol to SmaR modulates biofilm formation and virulence factor production by Serratia sp. These findings highlight the potential of eugenol as a promising agent to combat S. marcescens infections by targeting its virulence factors through quorum sensing inhibition. Competing Interests: Declaration of competing interest All authors have thoroughly reviewed and approved the manuscript, and we all concur with its submission to Microbial Pathogenesis journal. The authors declare that they have no competing interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Biofilm; Eugenol; HSL; Molecular docking; Molecular dynamic; Quorum sensing; Serratia; SmaR |
| المشرفين على المادة: | 3T8H1794QW (Eugenol) 0 (Virulence Factors) 0 (Anti-Bacterial Agents) |
| تواريخ الأحداث: | Date Created: 20240221 Date Completed: 20240320 Latest Revision: 20240320 |
| رمز التحديث: | 20240320 |
| DOI: | 10.1016/j.micpath.2024.106576 |
| PMID: | 38382626 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-1208 |
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| DOI: | 10.1016/j.micpath.2024.106576 |