دورية أكاديمية
Galectin-8 modulates human osteoclast activity partly through isoform-specific interactions.
| العنوان: | Galectin-8 modulates human osteoclast activity partly through isoform-specific interactions. |
|---|---|
| المؤلفون: | Roy M; https://ror.org/00kybxq39 Division of Rheumatology, Department of Medicine, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, Canada., Mbous Nguimbus L; https://ror.org/00kybxq39 Division of Rheumatology, Department of Medicine, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, Canada., Badiane PY; https://ror.org/00kybxq39 Division of Rheumatology, Department of Medicine, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, Canada., Goguen-Couture V; https://ror.org/00kybxq39 Division of Rheumatology, Department of Medicine, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, Canada., Degrandmaison J; https://ror.org/00kybxq39 Division of Rheumatology, Department of Medicine, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, Canada., Parent JL; https://ror.org/00kybxq39 Division of Rheumatology, Department of Medicine, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, Canada., Brunet MA; https://ror.org/00kybxq39 Department of Paediatrics, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, Canada., Roux S; https://ror.org/00kybxq39 Division of Rheumatology, Department of Medicine, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, Canada Sophie.Roux@USherbrooke.ca. |
| المصدر: | Life science alliance [Life Sci Alliance] 2024 Feb 23; Vol. 7 (5). Date of Electronic Publication: 2024 Feb 23 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Life Science Alliance, LLC Country of Publication: United States NLM ID: 101728869 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 2575-1077 (Electronic) Linking ISSN: 25751077 NLM ISO Abbreviation: Life Sci Alliance Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Woodbury, NY] : Life Science Alliance, LLC, [2018]- |
| مواضيع طبية MeSH: | Bone Resorption*/metabolism , Galectins*/genetics , Galectins*/metabolism , Osteoclasts*/metabolism, Humans ; Chloride Channels/metabolism ; Chromatography, Liquid ; HEK293 Cells ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; Proteomics ; Tandem Mass Spectrometry |
| مستخلص: | In overactive human osteoclasts, we previously identified an alternative splicing event in LGALS8 , encoding galectin-8, resulting in decreased expression of the long isoform. Galectin-8, which modulates cell-matrix interactions and functions intracellularly as a danger recognition receptor, has never been associated with osteoclast biology. In human osteoclasts, inhibition of galectin-8 expression revealed its roles in bone resorption, osteoclast nuclearity, and mTORC1 signaling regulation. Galectin-8 isoform-specific inhibition asserted a predominant role for the short isoform in bone resorption. Moreover, a liquid chromatography with tandem mass spectrometry (LC-MS/MS) proteomic analysis of galectin-8 isoforms performed in HEK293T cells identified 22 proteins shared by both isoforms. Meanwhile, nine interacting partners were specific for the short isoform, and none were unique to the long isoform. Interactors specific for the galectin-8 short isoform included cell adhesion proteins and lysosomal proteins. We confirmed the interactions of galectin-8 with CLCN3, CLCN7, LAMP1, and LAMP2, all known to localize to secretory vesicles, in human osteoclasts. Altogether, our study reveals direct roles of galectin-8 in osteoclast activity, mostly attributable to the short isoform. (© 2024 Roy et al.) |
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| المشرفين على المادة: | 0 (Chloride Channels) 0 (CLCN7 protein, human) 0 (Galectins) 0 (LGALS8 protein, human) 0 (Protein Isoforms) |
| تواريخ الأحداث: | Date Created: 20240223 Date Completed: 20240226 Latest Revision: 20240229 |
| رمز التحديث: | 20240229 |
| مُعرف محوري في PubMed: | PMC10895193 |
| DOI: | 10.26508/lsa.202302348 |
| PMID: | 38395460 |
| قاعدة البيانات: | MEDLINE |
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