دورية أكاديمية
أعرض في EDS

Decoupling the Effects of Collagen Alignment and Bioceramic Incorporation on Osteoblast Proliferation, Differentiation, and Mineralization.

التفاصيل البيبلوغرافية
العنوان: Decoupling the Effects of Collagen Alignment and Bioceramic Incorporation on Osteoblast Proliferation, Differentiation, and Mineralization.
المؤلفون: Patrawalla NY; Department of Biomedical Engineering and Sciences, Florida Institute of Technology, Melbourne, FL 32901., Bock K; Department of Biomedical Engineering and Sciences, Florida Institute of Technology, Melbourne, FL 32901., Liebendorfer K; Department of Biomedical Engineering and Sciences, Florida Institute of Technology, Melbourne, FL 32901., Kishore V; Department of Biomedical Engineering and Sciences, Florida Institute of Technology, Melbourne, FL 32901.; Department of Chemistry and Chemical Engineering, Florida Institute of Technology, Melbourne, FL 32901.
المصدر: Materials today. Communications [Mater Today Commun] 2024 Mar; Vol. 38. Date of Electronic Publication: 2024 Feb 07.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Ltd Country of Publication: England NLM ID: 101702545 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2352-4928 (Print) Linking ISSN: 23524928 NLM ISO Abbreviation: Mater Today Commun Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Oxford] : Elsevier Ltd., [2014]-
مستخلص: Biomimetic scaffolds provide the essential biophysical (e.g., surface topography, stiffness) and biochemical cues (e.g., composition) to guide cell morphology, proliferation, and differentiation. Although the effects of biomaterial-directed cues on cell response have been widely reported, few studies have sought to decouple these effects to better understand the interplay between the different physicochemical factors on tissue-specific cell function. Herein, beta-tricalcium phosphate (β-TCP) was incorporated into electrochemically aligned collagen (ELAC) and random collagen threads, and the individual and interactive effects of collagen alignment (i.e., biophysical) and bioceramic incorporation (i.e., biochemical) on osteoblast cell morphology, proliferation, differentiation, and mineralization were investigated. Results showed that collagen alignment in ELAC threads was retained upon β-TCP incorporation. Collagen alignment significantly improved (p < 0.05) the swelling capacity and stability of collagen threads, while β-TCP incorporation showed no such effects. Tensile tests revealed that β-TCP incorporation significantly decreased (p < 0.05) the strength and stiffness of ELAC threads. Significant increase (p < 0.05) in Saos-2 cell orientation and alkaline phosphatase (ALP) activity was observed on ELAC compared to random collagen threads indicating that aligned collagen serves as a key driving factor for osteogenesis. β-TCP incorporation into random collagen threads had no effect on Saos-2 cell function. On the other hand, presence of β-TCP significantly augmented (p < 0.05) Saos-2 cell metabolic activity, differentiation, and mineralization on ELAC threads. Together, these findings suggest that combining collagen alignment and β-TCP incorporation can create robust tissue-mimicking scaffolds for bone regeneration applications.
Competing Interests: Declaration of interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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معلومات مُعتمدة: R15 AR071102 United States AR NIAMS NIH HHS
فهرسة مساهمة: Keywords: Saos-2 cells; alignment; bioceramic; bone tissue engineering; collagen; differentiation; mineralization; osteoblasts; tricalcium phosphate
تواريخ الأحداث: Date Created: 20240226 Latest Revision: 20240228
رمز التحديث: 20240228
مُعرف محوري في PubMed: PMC10883576
DOI: 10.1016/j.mtcomm.2024.108329
PMID: 38405262
قاعدة البيانات: MEDLINE
الوصف
تدمد:2352-4928
DOI:10.1016/j.mtcomm.2024.108329