An Organism-Level Quantitative Flux Model of Mammalian Energy Metabolism.
| العنوان: | An Organism-Level Quantitative Flux Model of Mammalian Energy Metabolism. |
|---|---|
| المؤلفون: | Yuan B; Department of Molecular Metabolism, Harvard T. H. Chan School of Public Health, Boston, Massachusetts, USA., Inouye KE; Department of Molecular Metabolism, Harvard T. H. Chan School of Public Health, Boston, Massachusetts, USA.; Sabri Ülker Center for Metabolic Research, Department of Molecular Metabolism, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA., Hotamışlıgil GS; Department of Molecular Metabolism, Harvard T. H. Chan School of Public Health, Boston, Massachusetts, USA.; Sabri Ülker Center for Metabolic Research, Department of Molecular Metabolism, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA.; Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA., Hui S; Department of Molecular Metabolism, Harvard T. H. Chan School of Public Health, Boston, Massachusetts, USA.; Sabri Ülker Center for Metabolic Research, Department of Molecular Metabolism, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA.; Lead Contact. |
| المصدر: | BioRxiv : the preprint server for biology [bioRxiv] 2024 Feb 12. Date of Electronic Publication: 2024 Feb 12. |
| نوع المنشور: | Preprint |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: | Mammalian tissues feed on nutrients in the blood circulation. At the organism-level, mammalian energy metabolism comprises of oxidation, interconverting, storing and releasing of circulating nutrients. Though much is known about the individual processes and nutrients, a holistic and quantitative model describing these processes for all major circulating nutrients is lacking. Here, by integrating isotope tracer infusion, mass spectrometry, and isotope gas analyzer measurement, we developed a framework to systematically quantify fluxes through these processes for 10 major circulating energy nutrients in mice, resulting in an organism-level quantitative flux model of energy metabolism. This model revealed in wildtype mice that circulating nutrients' metabolic cycling fluxes are more dominant than their oxidation fluxes, with distinct partition between cycling and oxidation flux for individual circulating nutrients. Applications of this framework in obese mouse models showed on a per animal basis extensive elevation of metabolic cycling fluxes in ob/ob mice, but not in diet-induced obese mice. Thus, our framework describes quantitatively the functioning of energy metabolism at the organism-level, valuable for revealing new features of energy metabolism in physiological and disease conditions. Competing Interests: DECLARATION OF INTERESTS The authors declare no competing interests. |
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| معلومات مُعتمدة: | R00 DK117066 United States DK NIDDK NIH HHS |
| فهرسة مساهمة: | Keywords: circulating nutrients; energy metabolism; in vivo flux quantification; isotope tracing; obesity |
| تواريخ الأحداث: | Date Created: 20240226 Latest Revision: 20240301 |
| رمز التحديث: | 20240301 |
| مُعرف محوري في PubMed: | PMC10888810 |
| DOI: | 10.1101/2024.02.11.579776 |
| PMID: | 38405872 |
| قاعدة البيانات: | MEDLINE |
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