Quantitative evaluation of endometrium-expressed mRNAs for the purpose of discriminating between menstruation and traumatic vaginal injury in sexual assault cases.

التفاصيل البيبلوغرافية
العنوان:	Quantitative evaluation of endometrium-expressed mRNAs for the purpose of discriminating between menstruation and traumatic vaginal injury in sexual assault cases.
المؤلفون:	Akutsu T; First Department of Forensic Science, National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba, 277-0882, Japan. tomoko@nrips.go.jp., Minegishi S; Department of Forensic Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan., Watanabe K; First Department of Forensic Science, National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba, 277-0882, Japan., Toyomane K; First Department of Forensic Science, National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba, 277-0882, Japan., Yamagishi T; First Department of Forensic Science, National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba, 277-0882, Japan., Sakurada K; Department of Forensic Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
المصدر:	International journal of legal medicine [Int J Legal Med] 2024 Jul; Vol. 138 (4), pp. 1245-1254. Date of Electronic Publication: 2024 Feb 27.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Springer International Country of Publication: Germany NLM ID: 9101456 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1437-1596 (Electronic) Linking ISSN: 09379827 NLM ISO Abbreviation: Int J Legal Med Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Heidelberg, FRG : Springer International, c1990-
مواضيع طبية MeSH:	Menstruation* , Vagina/injuries , Matrix Metalloproteinase 7/genetics , RNA, Messenger* , Real-Time Polymerase Chain Reaction*, Humans ; Female ; Endometrium/metabolism ; Adult ; Biomarkers ; Young Adult ; Sex Offenses ; Cornified Envelope Proline-Rich Proteins/genetics ; Specimen Handling
مستخلص:	In sexual assault cases, it is crucial to discriminate between peripheral blood and menstrual blood to provide evidence for vaginal intercourse with traumatic injury. In this study, the menstrual blood mRNA markers progestagen-associated endometrial protein (PAEP), matrix metallopeptidase 7 (MMP7), and left-right determination factor 2 (LEFTY2) were evaluated by quantitative RT-PCR (RT-qPCR) for the discrimination of menstrual blood from peripheral blood and vaginal fluid. As a result, all markers with cutoff delta cycle quantification (ΔCq) values were specifically determined in menstrual blood among forensically relevant body fluids. Even though the changes in the expression levels of each marker differed during the menstrual cycle, all markers were determined to be positive in most of the randomly collected menstrual blood samples that were analyzed. Additionally, the markers with proposed cutoff ΔCq values could discriminate between menstrual blood and peripheral blood-mixed vaginal fluid samples. The determination of positive markers was less affected by storage temperature under dry conditions than under wet conditions, while PAEP was detectable in samples stored below room temperature under wet conditions. The detectability of PAEP was considered to be the result of its higher expression level compared with MMP7 and LEFTY2. In conclusion, menstrual blood markers for the RT-qPCR procedure evaluated in this study were highly specific for menstrual blood. The proposed procedure could be useful for discriminating between menstruation and traumatic bleeding in the female genital tract. In particular, PAEP is expected to be applicable to forensic casework samples because of its high specificity and robustness. (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
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معلومات مُعتمدة:	21K10538 Japan Society for the Promotion of Science
فهرسة مساهمة:	Keywords: Menstrual blood; Peripheral blood; RT-qPCR; Sexual assault; mRNA
المشرفين على المادة:	EC 3.4.24.23 (Matrix Metalloproteinase 7) 0 (RNA, Messenger) 0 (Biomarkers) EC 3.4.24.23 (MMP7 protein, human) 0 (Cornified Envelope Proline-Rich Proteins)
تواريخ الأحداث:	Date Created: 20240227 Date Completed: 20240610 Latest Revision: 20240610
رمز التحديث:	20240611
DOI:	10.1007/s00414-024-03199-y
PMID:	38409607
قاعدة البيانات:	MEDLINE

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تدمد:	1437-1596
DOI:	10.1007/s00414-024-03199-y