دورية أكاديمية
Protocol for Testing Human Melanoma Exosomes that Shift the Healthy Phenotype of Human Dermal Cells.
| العنوان: | Protocol for Testing Human Melanoma Exosomes that Shift the Healthy Phenotype of Human Dermal Cells. |
|---|---|
| المؤلفون: | Somuncu ÖS; Department of Biotechnology, Yeditepe University, Istanbul, Turkey. ozges_somuncu@dfci.harvard.edu.; Department of Radiation Oncology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA, USA. ozges_somuncu@dfci.harvard.edu.; Center for DNA Damage and Repair, Dana-Farber Cancer Institute, Boston, MA, USA. ozges_somuncu@dfci.harvard.edu., Taşli PN; Department of Biotechnology, Yeditepe University, Istanbul, Turkey., Şahin F; Department of Biotechnology, Yeditepe University, Istanbul, Turkey. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2849, pp. 149-160. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Exosomes*/metabolism , Melanoma*/metabolism , Melanoma*/pathology , Phenotype* , Fibroblasts*/metabolism, Humans ; Cell Line, Tumor ; Epithelial-Mesenchymal Transition/genetics ; rab27 GTP-Binding Proteins/metabolism ; rab27 GTP-Binding Proteins/genetics ; Dermis/cytology ; Dermis/metabolism ; Dermis/pathology |
| مستخلص: | Exosomes are small membrane-derived vesicles that transmit DNA constituents, mRNAs, microRNAs, and proteins from donor cells to a receiver cell. Various cells comprising of mesenchymal, immune, and cancer cells discharge exosomes. Cancer cell exosomes form the entry and reprogramming of essentials connected to a tumor environment. Melanoma-derived exosomes transport diverse proteins such as c-MET and RAB27a, which leave a melanoma mark. Increased mesenchymal epithelial transition (MET) expressions in serum exosomes have been considered an indicator of disease progression. Meanwhile, RAB27a has been identified as being involved in exosome discharge and trafficking. Decreased expressions of RAB27a in human melanoma cells have shown to diminish exosome release.We examined the effects of the downregulation and upregulation of RAB27a and c-MET in human dermal fibroblasts by utilizing the isolated exosomes of malignant melanoma cell lines. Melanoma exosomes derived from cancer cells conveyed information to healthy dermal fibroblasts and stem cells while inducing phenotypic change. In this chapter, we show optimized protocols that were used by our group for in vitro analysis with melanoma exosomes. (© 2024. Springer Science+Business Media, LLC.) |
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| فهرسة مساهمة: | Keywords: Exosome isolation; Exosomes; Melanoma exosomes; RAB27a; c-MET |
| المشرفين على المادة: | 0 (rab27 GTP-Binding Proteins) EC 3.6.1.-. (RAB27A protein, human) |
| تواريخ الأحداث: | Date Created: 20240227 Date Completed: 20240719 Latest Revision: 20240719 |
| رمز التحديث: | 20240719 |
| DOI: | 10.1007/7651_2024_519 |
| PMID: | 38411889 |
| قاعدة البيانات: | MEDLINE |
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