دورية أكاديمية
Quality control on oil palm RNA samples for efficient genomic downstream applications.
| العنوان: | Quality control on oil palm RNA samples for efficient genomic downstream applications. |
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| المؤلفون: | Apryianto A; Biopolymer Analytics, Institute of Biochemistry and Biology, University of Potsdam, Karl- liebknecht-str. 24-25 Potsdam Golm- Germany.; Laboratprium Bioteknologi, ASTRA Agro Lestari Ltd Indonesia., Arsela P; Prodi Agroteknologi, Fakultas Pertanian dan bisnis Digital, Universitas Muhammadiyah Kalimantan Timur, Indonesia., Felix F; Institute of Agricultural Research for Development (IRAD), Cameroon., Ajambang W; Institute of Agricultural Research for Development (IRAD), Cameroon.; Laboratprium Bioteknologi, ASTRA Agro Lestari Ltd Indonesia.; Prodi Agroteknologi, Fakultas Pertanian dan bisnis Digital, Universitas Muhammadiyah Kalimantan Timur, Indonesia. |
| المصدر: | Journal of genomics [J Genomics] 2024 Feb 17; Vol. 12, pp. 35-43. Date of Electronic Publication: 2024 Feb 17 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Ivyspring International Publisher Country of Publication: Australia NLM ID: 101623535 Publication Model: eCollection Cited Medium: Print ISSN: 1839-9940 (Print) NLM ISO Abbreviation: J Genomics Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: [Sydney, NSW, Australia] : Ivyspring International Publisher, [2013]- |
| مستخلص: | Quality control (QC) is primordial for determining the efficiency in any downstream genomic applications. There are several steps in the verification of the quality of RNA samples destined for genomic studies. The aim of this research was to determine whether RNA should be discarded at the level of the field lab if it fails preliminary quality control using Optical Density (OD) measurements. In this study, all samples were submitted to rigorous quality control in every stage of work. RNA samples showing poor OD values still gave excellent results in downstream QC and genomic applications. At the end of the quality control exercise, it was observed that the original samples were the same and had not undergone any deterioration along the different stages of handling and manipulation. This paper shows the different and most important stages of quality control on RNA samples (RIN) for an effective down stream application in genomic studies. RNA samples should not be discarded based on preliminary QC from our field labs. Competing Interests: Competing Interests: The authors have declared that no competing interest exists. (© The author(s).) |
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| فهرسة مساهمة: | Keywords: OD; RIN; RNA; genomics; oil palm |
| تواريخ الأحداث: | Date Created: 20240304 Latest Revision: 20240813 |
| رمز التحديث: | 20240813 |
| مُعرف محوري في PubMed: | PMC10905253 |
| DOI: | 10.7150/jgen.92209 |
| PMID: | 38434105 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1839-9940 |
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| DOI: | 10.7150/jgen.92209 |