دورية أكاديمية
Untargeted metabolomics unveiled the role of butanoate metabolism in the development of Pseudomonas aeruginosa hypoxic biofilm.
| العنوان: | Untargeted metabolomics unveiled the role of butanoate metabolism in the development of Pseudomonas aeruginosa hypoxic biofilm. |
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| المؤلفون: | Abdelhamid AG; Department of Food Science and Technology, The Ohio State University, Columbus, OH, United States.; Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt., Yousef AE; Department of Food Science and Technology, The Ohio State University, Columbus, OH, United States.; Department of Microbiology, The Ohio State University, Columbus, OH, United States. |
| المصدر: | Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2024 Feb 16; Vol. 14, pp. 1346813. Date of Electronic Publication: 2024 Feb 16 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Media SA |
| مواضيع طبية MeSH: | Pseudomonas aeruginosa* , Acetoin*, Humans ; Metabolomics ; Metabolome ; Hypoxia ; Biofilms |
| مستخلص: | Pseudomonas aeruginosa is a versatile opportunistic pathogen which causes a variety of acute and chronic human infections, some of which are associated with the biofilm phenotype of the pathogen. We hypothesize that defining the intracellular metabolome of biofilm cells, compared to that of planktonic cells, will elucidate the metabolic pathways and biomarkers indicative of biofilm inception. Disc-shaped stainless-steel coupons (12.7 mm diameter) were employed as a surface for static biofilm establishment. Each disc was immersed in a well, of a 24-well microtiter plate, containing a 1-mL Lysogeny broth (LB) suspension of P. aeruginosa ATCC 9027, a strain known for its biofilm prolificacy. This setup underwent oxygen-depleted incubation at 37°C for 24 hours to yield hypoxic biofilms and the co-existing static planktonic cells. In parallel, another planktonic phenotype of ATCC 9027 was produced in LB under shaking (200 rpm) incubation at 37°C for 24 hours. Planktonic and biofilm cells were harvested, and the intracellular metabolites were subjected to global untargeted metabolomic analysis using LC-MS technology, where small metabolites (below 1.5 kDa) were selected. Data analysis showed the presence of 324 metabolites that differed ( p < 0.05) in abundance between planktonic and biofilm cells, whereas 70 metabolites did not vary between these phenotypes ( p > 0.05). Correlation, principal components, and partial least square discriminant analyses proved that the biofilm metabolome is distinctly clustered away from that of the two planktonic phenotypes. Based on the functional enrichment analysis, arginine and proline metabolism were enriched in planktonic cells, but butanoate metabolism was enriched in biofilm cells. Key differential metabolites within the butanoate pathway included acetoacetate, 2,3-butandiol, diacetyl, and acetoin, which were highly upregulated in the biofilm compared to the planktonic cells. Exogenous supplementation of acetoin (2 mM), a critical metabolite in butanoate metabolism, augmented biofilm mass, increased the structural integrity and thickness of the biofilm, and maintained the intracellular redox potential by balancing NADH/NAD + ratio. In conclusion, P. aeruginosa hypoxic biofilm has a specialized metabolic landscape, and butanoate pathway is a metabolic preference and possibly required for promoting planktonic cells to the biofilm state. The butanoate pathway metabolites, particularly acetoin, could serve as markers for biofilm development. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Abdelhamid and Yousef.) |
| References: | Methods Mol Biol. 2014;1149:631-41. (PMID: 24818938) ISME J. 2020 Dec;14(12):3093-3105. (PMID: 32814867) Cell Host Microbe. 2020 Mar 11;27(3):345-357.e6. (PMID: 32078802) Diagn Microbiol Infect Dis. 2017 Jun;88(2):152-157. (PMID: 28366610) Trends Microbiol. 2019 Oct;27(10):850-863. (PMID: 31178124) J Proteome Res. 2019 Jun 7;18(6):2601-2612. (PMID: 31060355) Microb Biotechnol. 2009 Jan;2(1):62-74. (PMID: 21261882) J Hazard Mater. 2022 Jan 15;422:126836. (PMID: 34403940) Drug Resist Updat. 2019 May;44:100640. (PMID: 31492517) mBio. 2018 Apr 10;9(2):. (PMID: 29636437) Int J Mol Sci. 2021 Mar 18;22(6):. (PMID: 33803907) J Appl Microbiol. 2006 Nov;101(5):1087-95. (PMID: 17040232) Dev Cell. 2002 Oct;3(4):593-603. (PMID: 12408810) J Bacteriol. 2005 Dec;187(23):8114-26. (PMID: 16291684) J Oral Sci. 2019;61(2):255-263. (PMID: 31217374) AMB Express. 2020 May 6;10(1):85. (PMID: 32378141) J Breath Res. 2016 Aug 17;10(3):036013. (PMID: 27532768) Nat Rev Microbiol. 2021 May;19(5):331-342. (PMID: 33214718) Signal Transduct Target Ther. 2022 Jun 25;7(1):199. (PMID: 35752612) Biotechnol Adv. 2021 Jul-Aug;49:107734. (PMID: 33785375) Int J Food Microbiol. 2018 Feb 21;267:9-19. (PMID: 29275280) Appl Environ Microbiol. 2014 Nov;80(21):6843-52. (PMID: 25172858) Front Cell Infect Microbiol. 2022 Feb 14;12:833269. (PMID: 35237533) BMC Genomics. 2006 Jun 08;7:142. (PMID: 16762068) Nucleic Acids Res. 2021 Jul 2;49(W1):W388-W396. (PMID: 34019663) Antibiotics (Basel). 2019 Dec 10;8(4):. (PMID: 31835470) PLoS Pathog. 2009 Apr;5(4):e1000382. (PMID: 19360133) Nucleic Acids Res. 2018 Jan 4;46(D1):D575-D580. (PMID: 29106626) Front Microbiol. 2017 Nov 27;8:2335. (PMID: 29230206) Sci Rep. 2022 Oct 15;12(1):17317. (PMID: 36243882) |
| فهرسة مساهمة: | Keywords: Pseudomonas aeruginosa; acetoin; bacterial biofilm; butanoate metabolism; hypoxic biofilm; in vitro biofilm; metabolic markers; metabolomics |
| المشرفين على المادة: | BG4D34CO2H (Acetoin) |
| تواريخ الأحداث: | Date Created: 20240304 Date Completed: 20240305 Latest Revision: 20240305 |
| رمز التحديث: | 20240305 |
| مُعرف محوري في PubMed: | PMC10904581 |
| DOI: | 10.3389/fcimb.2024.1346813 |
| PMID: | 38435305 |
| قاعدة البيانات: | MEDLINE |
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