دورية أكاديمية
Particulate matters 2.5 induce tumor progression in lung cancer by increasing the activity of hnRNPA2B1 resulting in retarding mRNA decay of oxidative phosphorylation.
| العنوان: | Particulate matters 2.5 induce tumor progression in lung cancer by increasing the activity of hnRNPA2B1 resulting in retarding mRNA decay of oxidative phosphorylation. |
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| المؤلفون: | Bian W; Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China., Yu H; Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China., Zhang X; Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China., Wang Y; Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China., Ni B; Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China. |
| المصدر: | IUBMB life [IUBMB Life] 2024 Aug; Vol. 76 (8), pp. 563-576. Date of Electronic Publication: 2024 Mar 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published for the International Union of Biochemistry and Molecular Biology by Taylor & Francis Country of Publication: England NLM ID: 100888706 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-6551 (Electronic) Linking ISSN: 15216543 NLM ISO Abbreviation: IUBMB Life Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; Philadelphia, PA : Published for the International Union of Biochemistry and Molecular Biology by Taylor & Francis, c1999- |
| مواضيع طبية MeSH: | Apoptosis* , Heterogeneous-Nuclear Ribonucleoprotein Group A-B*/metabolism , Heterogeneous-Nuclear Ribonucleoprotein Group A-B*/genetics , Lung Neoplasms*/pathology , Lung Neoplasms*/genetics , Lung Neoplasms*/metabolism , Oxidative Phosphorylation* , Particulate Matter*/adverse effects , RNA Stability*, Animals ; Humans ; Mice ; A549 Cells ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Disease Progression ; Gene Expression Regulation, Neoplastic ; Mice, Inbred BALB C ; Mice, Nude ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Xenograft Model Antitumor Assays |
| مستخلص: | Particulate matter 2.5 (PM2.5) has been implicated in lung injury and various cancers, yet its precise mechanistic role remains elusive. To elucidate the key signaling pathways underpinning PM2.5-induced lung cancer progression, we embarked on a study examining the impact of PM2.5 both in vitro and in vivo. Lung cancer cell lines, A549 and H157, were employed for the in vitro investigations. Overexpression or knockdown techniques targeting the hnRNPA2B1 protein were implemented. Lung cancer cells were treated with a medium containing PM2.5 and subsequently prepared for in vitro evaluations. Cell growth, invasion, and migration were gauged using transwell and CCK-8 assays. Apoptosis was ascertained through flow cytometry and western blotting of pertinent proteins. Seahorse analyses probed the influence of PM2.5 on lung cancer energy metabolism. The RNA stability assay was employed to discern the impact of PM2.5 on the stability of oxidative phosphorylation-related genes in lung cancer. Our findings revealed that PM2.5 augmented cell proliferation, migration, and invasion rates. Similarly, a diminished apoptosis rate was observed in PM2.5-treated cells. Elevated expression of hnRNPA2B1 was detected in lung cancer cells exposed to PM2.5. Moreover, in cells treated with PM2.5, hnRNPA2B1 knockdown markedly curtailed cell proliferation by inducing G1-S cell cycle arrest and bolstered lung cancer cell apoptosis in vitro; it also curbed xenograft tumor growth. Mechanistically, our data suggest that PM2.5 undermines the stability of mRNA transcripts associated with oxidative phosphorylation (OXPHOS) and augments the formation of processing bodies (P-bodies), leading to an upsurge in OXPHOS levels. In conclusion, PM2.5 appears to drive lung cancer progression and migration by modulating the energy metabolism of lung cancer in a hnRNPA2B1-dependent manner. (© 2024 International Union of Biochemistry and Molecular Biology.) |
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| فهرسة مساهمة: | Keywords: RNPA2B1; energy metabolism; lung cancer; oxidative phosphorylation; particulate matter |
| المشرفين على المادة: | 0 (Heterogeneous-Nuclear Ribonucleoprotein Group A-B) 0 (hnRNP A2) 0 (Particulate Matter) 0 (RNA, Messenger) |
| تواريخ الأحداث: | Date Created: 20240307 Date Completed: 20240731 Latest Revision: 20240814 |
| رمز التحديث: | 20240814 |
| DOI: | 10.1002/iub.2813 |
| PMID: | 38450584 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1521-6551 |
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| DOI: | 10.1002/iub.2813 |