دورية أكاديمية
Phenogenetic profile and agronomic contribution of Azospirillum argentinense Az39 T , a reference strain for the South American inoculant industry.
| العنوان: | Phenogenetic profile and agronomic contribution of Azospirillum argentinense Az39 T , a reference strain for the South American inoculant industry. |
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| المؤلفون: | Maroniche GA; Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata (UNMdP), CONICET, Balcarce, Buenos Aires, Argentina., Puente ML; Instituto de Microbiología y Zoología Agrícola, Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina., García JE; Instituto de Microbiología y Zoología Agrícola, Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina., Mongiardini E; Instituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP), CONICET, La Plata, Buenos Aires, Argentina., Coniglio A; Laboratorio de Fisiología Vegetal y de la Interacción Planta-Microorganismo, Instituto de Investigaciones Agrobiotecnológicas (INIAB-CONICET), Universidad Nacional de Río Cuarto (UNRC), Río Cuarto, Córdoba, Argentina., Nievas S; Laboratorio de Fisiología Vegetal y de la Interacción Planta-Microorganismo, Instituto de Investigaciones Agrobiotecnológicas (INIAB-CONICET), Universidad Nacional de Río Cuarto (UNRC), Río Cuarto, Córdoba, Argentina., Labarthe MM; Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata (UNMdP), CONICET, Balcarce, Buenos Aires, Argentina., Wisniewski-Dyé F; Universite Claude Bernard Lyon 1, Laboratoire d'Ecologie Microbienne, UMR CNRS 5557, UMR INRAE 1418, VetAgro Sup, Villeurbanne 69622, France., Rodriguez Cáceres E; Plantec Biotecnología, Buenos Aires, Argentina., Díaz-Zorita M; Facultad de Agronomía, Universidad Nacional de La Pampa (UNLPam), CONICET, Santa Rosa, La Pampa, Argentina., Cassán F; Laboratorio de Fisiología Vegetal y de la Interacción Planta-Microorganismo, Instituto de Investigaciones Agrobiotecnológicas (INIAB-CONICET), Universidad Nacional de Río Cuarto (UNRC), Río Cuarto, Córdoba, Argentina. Electronic address: fcassan@exa.unrc.edu.ar. |
| المصدر: | Microbiological research [Microbiol Res] 2024 Jun; Vol. 283, pp. 127650. Date of Electronic Publication: 2024 Feb 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: G. Fischer Country of Publication: Germany NLM ID: 9437794 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-0623 (Electronic) Linking ISSN: 09445013 NLM ISO Abbreviation: Microbiol Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Jena : G. Fischer, c1994- |
| مواضيع طبية MeSH: | Azospirillum*/physiology, Indoleacetic Acids/metabolism ; Plant Development ; Carbon ; South America |
| مستخلص: | Azospirillum sp. is a plant growth-promoting rhizobacteria largely recognized for its potential to increase the yield of different important crops. In this work, we present a thorough genomic and phenotypic analysis of A. argentinense Az39 T to provide new insights into the beneficial mechanisms of this microorganism. Phenotypic analyses revealed the following in vitro abilities: growth at 20-38 °C (optimum, 28 °C), pH 6.0-8.0 (optimum, pH 6.8), and in the presence of 1% (w/v) NaCl; production of variable amounts of PHB as intracellular granules; nitrogen fixation under microaerophilic conditions; IAA synthesis in the presence of L-tryptophan. Through biochemical (API 20NE) and carbon utilization profiling (Biolog) assays, we proved that A. argentinense Az39 T is able to use 15 substrates and metabolize 19 different carbon substrates. Lipid composition indicated a predominance of medium and long-chain saturated fatty acids. A total of 6 replicons classified as one main chromosome, three chromids, and two plasmids, according to their tRNA and core essential genes contents, were identified. Az39 T genome includes genes associated with multiple plant growth-promoting (PGP) traits such as nitrogen fixation and production of auxins, cytokinin, abscisic acid, ethylene, and polyamines. In addition, Az39 T genome harbor genetic elements associated with physiological features that facilitate its survival in the soil and competence for rhizospheric colonization; this includes motility, secretion system, and quorum sensing genetic determinants. A metadata analysis of Az39 T agronomic performance in the pampas region, Argentina, demonstrated significant grain yield increases in wheat and maize, proving its potential to provide better growth conditions for dryland cereals. In conclusion, our data provide a detailed insight into the metabolic profile of A. argentinense Az39 T , the strain most widely used to formulate non-legume inoculants in Argentina, and allow a better understanding of the mechanisms behind its field performance. Competing Interests: Declaration of Competing Interest The authors report no conflicts of interest. (Copyright © 2024 Elsevier GmbH. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Azospirillum argentinense Az39(T) genome; Crop yield improvement; Inoculants; Plant growth-promoting mechanisms; Rhizosphere colonization |
| المشرفين على المادة: | 0 (Indoleacetic Acids) 7440-44-0 (Carbon) |
| تواريخ الأحداث: | Date Created: 20240307 Date Completed: 20240417 Latest Revision: 20240417 |
| رمز التحديث: | 20240417 |
| DOI: | 10.1016/j.micres.2024.127650 |
| PMID: | 38452553 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1618-0623 |
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| DOI: | 10.1016/j.micres.2024.127650 |