دورية أكاديمية
Aqueous Ozone Exposure Inhibits Sporulation in the Cyclospora cayetanensis Surrogate Eimeria acervulina.
| العنوان: | Aqueous Ozone Exposure Inhibits Sporulation in the Cyclospora cayetanensis Surrogate Eimeria acervulina. |
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| المؤلفون: | Baumann AA; Center for Agricultural Synthetic Biology (CASB), University of Tennessee, Knoxville, TN, USA., Myers AK; Department of Food Science, University of Tennessee, Knoxville, TN, USA., Khajeh-Kazerooni N; Center for Agricultural Synthetic Biology (CASB), University of Tennessee, Knoxville, TN, USA., Rosenthal B; Animal Parasitic Disease Laboratory, Agricultural Research Service, US Department of Agriculture, Beltsville, MD, USA., Jenkins M; Animal Parasitic Disease Laboratory, Agricultural Research Service, US Department of Agriculture, Beltsville, MD, USA., O'Brien C; Animal Parasitic Disease Laboratory, Agricultural Research Service, US Department of Agriculture, Beltsville, MD, USA., Fuller L; Department of Poultry Science, University of Georgia, Athens, GA, USA., Morgan M; Department of Food Science, University of Tennessee, Knoxville, TN, USA., Lenaghan SC; Center for Agricultural Synthetic Biology (CASB), University of Tennessee, Knoxville, TN, USA; Department of Food Science, University of Tennessee, Knoxville, TN, USA. Electronic address: slenagha@utk.edu. |
| المصدر: | Journal of food protection [J Food Prot] 2024 May; Vol. 87 (5), pp. 100260. Date of Electronic Publication: 2024 Mar 08. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 7703944 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1944-9097 (Electronic) Linking ISSN: 0362028X NLM ISO Abbreviation: J Food Prot Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : [New York, NY] : Elsevier Original Publication: Ames, Iowa, International Association of Milk, Food, and Environmental Sanitarians. |
| مواضيع طبية MeSH: | Cyclospora*/drug effects , Eimeria*/drug effects , Ozone*/pharmacology , Oocysts*/drug effects, Animals ; Humans ; Water ; Cyclosporiasis ; Disinfectants/pharmacology |
| مستخلص: | Ozone is a potent disinfecting agent used to treat potable water and wastewater, effectively clearing protozoa such as Giardia and Cryptosporidium spp. It is unclear whether ozone treatment of water or fresh produce can reduce the spread of the emerging parasite Cyclospora cayetanensis, which causes cyclosporiasis in humans. Obtaining viable C. cayetanensis oocysts to evaluate inactivation methods is challenging because we lack the means to propagate them in vitro, because of delays in case reporting, and because health departments typically add inactivating fixatives to clinical specimens. Research in various surrogate organisms has sought to bolster understanding of the biology of C. cayetanensis. Among these surrogates is the poultry parasite Eimeria acervulina, a closely related and easily cultured parasite of economic significance. We used this surrogate to evaluate the consequences of ozone treatment, using the sporulation state as an indicator of infectious potential. Treating with ozonated water acidified with citric acid reduced sporulation ability in a dose-dependent manner; treatment with up to 4.93 mg/L initial concentration of ozone resulted in a 93% inactivation of sporulation by 7 days posttreatment. This developmental arrest was accompanied by transcriptional changes in genes involved in regulating the response to reactive oxygen species (ROS) in a time course that is consistent with the production of oxygen free radicals. This study shows that ozone is highly effective in preventing sporulation of E. acervulina, a model coccidian used as a surrogate for Cyclospora. Furthermore, ozone exposure induced molecular responses to general oxidative stress, documented with several well-characterized antioxidant enzymes. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cyclospora; Eimeria; Oxidative stress; Ozone; Sporulation |
| المشرفين على المادة: | 66H7ZZK23N (Ozone) 059QF0KO0R (Water) 0 (Disinfectants) |
| تواريخ الأحداث: | Date Created: 20240309 Date Completed: 20240427 Latest Revision: 20240427 |
| رمز التحديث: | 20240428 |
| DOI: | 10.1016/j.jfp.2024.100260 |
| PMID: | 38460785 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1944-9097 |
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| DOI: | 10.1016/j.jfp.2024.100260 |