دورية أكاديمية
أعرض في EDS

Proteomics study of bone tissue around ameloblastoma and the potential mechanism of CD36 in bone remodelling.

التفاصيل البيبلوغرافية
العنوان: Proteomics study of bone tissue around ameloblastoma and the potential mechanism of CD36 in bone remodelling.
المؤلفون: Zhang CX; State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, China., Man QW; State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, China; Department of Oral & Maxillofacial - Head Neck Oncology, School & Hospital of Stomatology, Wuhan University, Wuhan, China. Electronic address: manqiwen@whu.edu.cn.
المصدر: The British journal of oral & maxillofacial surgery [Br J Oral Maxillofac Surg] 2024 Apr; Vol. 62 (3), pp. 290-298. Date of Electronic Publication: 2024 Jan 04.
نوع المنشور: Journal Article; Comparative Study
اللغة: English
بيانات الدورية: Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 8405235 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-1940 (Electronic) Linking ISSN: 02664356 NLM ISO Abbreviation: Br J Oral Maxillofac Surg Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Edinburgh, U.K. : Churchill Livingstone, c1984-
مواضيع طبية MeSH: Ameloblastoma*/metabolism , Ameloblastoma*/pathology , Bone Remodeling*/physiology , Proteomics* , CD36 Antigens*/metabolism , CD36 Antigens*/analysis, Humans ; Mandibular Neoplasms/metabolism ; Mandibular Neoplasms/pathology ; Chromatography, Liquid ; Cancellous Bone/metabolism ; Lipid Metabolism/physiology ; Adult ; Female ; Male ; Mandible/metabolism ; Mass Spectrometry ; Fatty Acids/metabolism ; Middle Aged ; Proteome/analysis
مستخلص: Ameloblastoma (AM) is characterised by local aggressiveness and bone resorption. To our knowledge, the proteomic profile of bone adjacent to AM has not previously been explored. We therefore looked at the differential proteins in cancellous bone (CB) adjacent to AM and normal CB from the mandible. CB proteins were extracted, purified, quantified, and analysed by liquid chromatography-mass spectrometry (LC-MS) using samples from five patients with AM. These proteins were further investigated using gene ontology for additional functional annotation and enrichment. Proteins that met the screening requirements of expression difference ploidy > 1.5-fold (upregulation and downregulation) and p < 0.05 were subsequently deemed differential proteins. Immunohistochemical staining was performed to confirm the above findings. Compared with normal mandibular CB, 151 differential proteins were identified in CB adjacent to the mandibular AM. These were mainly linked to cellular catabolic processes, lipid metabolism, and fatty acids (FA) metabolism. LC-MS and immunohistochemistry showed that CD36 was one of the notably decreased proteins in CB bordering the AM compared with normal mandibular CB (p = 0.0066 and p = 0.0095, respectively). CD36 expression in CB correlates with bone remodelling in AM, making CD36 a viable target for therapeutic approaches.
(Copyright © 2024 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: ameloblastoma; bone; metabolism; proteomics
المشرفين على المادة: 0 (CD36 Antigens)
0 (Fatty Acids)
0 (CD36 protein, human)
0 (Proteome)
تواريخ الأحداث: Date Created: 20240309 Date Completed: 20240502 Latest Revision: 20240521
رمز التحديث: 20240521
DOI: 10.1016/j.bjoms.2024.01.001
PMID: 38461076
قاعدة البيانات: MEDLINE
كن أول من يترك تعليقا!
يجب تسجيل دخولك أولا