دورية أكاديمية
أعرض في EDS

N 6 -methyladenosine modification is not a general trait of viral RNA genomes.

التفاصيل البيبلوغرافية
العنوان: N 6 -methyladenosine modification is not a general trait of viral RNA genomes.
المؤلفون: Baquero-Pérez B; Molecular Virology Group, Department of Medicine and Life Sciences, Universitat Pompeu Fabra, Dr. Aiguader 88, 08003, Barcelona, Spain., Yonchev ID; Sheffield Institute for Nucleic Acids (SInFoNiA) and School of Biosciences, The University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK.; Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA., Delgado-Tejedor A; Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, 08003, Barcelona, Spain., Medina R; Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, 08003, Barcelona, Spain., Puig-Torrents M; Molecular Virology Group, Department of Medicine and Life Sciences, Universitat Pompeu Fabra, Dr. Aiguader 88, 08003, Barcelona, Spain., Sudbery I; Sheffield Institute for Nucleic Acids (SInFoNiA) and School of Biosciences, The University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK., Begik O; Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, 08003, Barcelona, Spain., Wilson SA; Sheffield Institute for Nucleic Acids (SInFoNiA) and School of Biosciences, The University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK. stuart.wilson@sheffield.ac.uk., Novoa EM; Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, 08003, Barcelona, Spain. eva.novoa@crg.eu.; Universitat Pompeu Fabra (UPF), 08003, Barcelona, Spain. eva.novoa@crg.eu., Díez J; Molecular Virology Group, Department of Medicine and Life Sciences, Universitat Pompeu Fabra, Dr. Aiguader 88, 08003, Barcelona, Spain. juana.diez@upf.edu.
المصدر: Nature communications [Nat Commun] 2024 Mar 11; Vol. 15 (1), pp. 1964. Date of Electronic Publication: 2024 Mar 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Chikungunya virus*/genetics , Dengue Virus*/genetics , Dengue* , Chikungunya Fever*, Adenosine/*analogs & derivatives, Humans ; RNA, Viral/genetics ; Antibodies, Viral
مستخلص: Despite the nuclear localization of the m 6 A machinery, the genomes of multiple exclusively-cytoplasmic RNA viruses, such as chikungunya (CHIKV) and dengue (DENV), are reported to be extensively m 6 A-modified. However, these findings are mostly based on m 6 A-Seq, an antibody-dependent technique with a high rate of false positives. Here, we address the presence of m 6 A in CHIKV and DENV RNAs. For this, we combine m 6 A-Seq and the antibody-independent SELECT and nanopore direct RNA sequencing techniques with functional, molecular, and mutagenesis studies. Following this comprehensive analysis, we find no evidence of m 6 A modification in CHIKV or DENV transcripts. Furthermore, depletion of key components of the host m 6 A machinery does not affect CHIKV or DENV infection. Moreover, CHIKV or DENV infection has no effect on the m 6 A machinery's localization. Our results challenge the prevailing notion that m 6 A modification is a general feature of cytoplasmic RNA viruses and underscore the importance of validating RNA modifications with orthogonal approaches.
(© 2024. The Author(s).)
References: Cell Res. 2017 Mar;27(3):444-447. (PMID: 28106076)
Nucleic Acids Res. 2021 Sep 20;49(16):e92. (PMID: 34157120)
Cell. 2013 Dec 5;155(6):1409-21. (PMID: 24269006)
Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772)
Nat Commun. 2023 Jan 19;14(1):334. (PMID: 36658122)
Genome Res. 2020 Sep;30(9):1345-1353. (PMID: 32907883)
Trends Genet. 2020 Jan;36(1):44-52. (PMID: 31810533)
Nucleic Acids Res. 2020 Jan 8;48(D1):D440-D444. (PMID: 31691833)
EMBO J. 2021 Feb 1;40(3):e105977. (PMID: 33470439)
Elife. 2017 Oct 31;6:. (PMID: 29087293)
Nat Methods. 2015 Aug;12(8):767-72. (PMID: 26121403)
Nature. 2017 Nov 9;551(7679):251-255. (PMID: 29072297)
Bioinformatics. 2009 Jul 15;25(14):1754-60. (PMID: 19451168)
Nature. 2020 Jul;583(7817):638-643. (PMID: 32555463)
Sci Rep. 2020 Apr 20;10(1):6590. (PMID: 32313079)
Cell Host Microbe. 2016 Nov 9;20(5):654-665. (PMID: 27773535)
Cell. 2020 May 14;181(4):914-921.e10. (PMID: 32330414)
PLoS Pathog. 2018 Apr 16;14(4):e1006995. (PMID: 29659627)
Cell. 2015 Jun 4;161(6):1388-99. (PMID: 26046440)
Proc Natl Acad Sci U S A. 2023 Apr 11;120(15):e2217053120. (PMID: 37011200)
Genome Res. 2010 Sep;20(9):1297-303. (PMID: 20644199)
Viruses. 2023 Jun 16;15(6):. (PMID: 37376680)
Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404)
Elife. 2017 Oct 06;6:. (PMID: 28984244)
Annu Rev Biochem. 2023 Jun 20;92:145-173. (PMID: 37068770)
Cell Host Microbe. 2017 Sep 13;22(3):377-386.e5. (PMID: 28910636)
Nat Microbiol. 2018 Jan;3(1):108-120. (PMID: 29109479)
Trends Biotechnol. 2021 Jan;39(1):72-89. (PMID: 32620324)
Nat Commun. 2020 Nov 26;11(1):6016. (PMID: 33243990)
Cell. 2017 Apr 6;169(2):326-337.e12. (PMID: 28388414)
PLoS Pathog. 2015 Nov 20;11(11):e1005274. (PMID: 26587836)
Nat Methods. 2021 Oct;18(10):1213-1222. (PMID: 34594034)
Nat Chem Biol. 2016 May;12(5):311-6. (PMID: 26863410)
Nat Rev Mol Cell Biol. 2019 Oct;20(10):608-624. (PMID: 31520073)
Nat Commun. 2021 Dec 10;12(1):7198. (PMID: 34893601)
Mol Cell. 2016 Feb 18;61(4):507-519. (PMID: 26876937)
Angew Chem Int Ed Engl. 2018 Dec 3;57(49):15995-16000. (PMID: 30345651)
Proc Natl Acad Sci U S A. 1975 Jan;72(1):318-22. (PMID: 164018)
Sci Rep. 2019 Jun 18;9(1):8697. (PMID: 31213632)
RNA. 2017 Oct;23(10):1493-1501. (PMID: 28724534)
Cell Res. 2014 Feb;24(2):177-89. (PMID: 24407421)
Cell. 2020 Jun 25;181(7):1582-1595.e18. (PMID: 32492408)
Mol Cell. 2020 Feb 6;77(3):542-555.e8. (PMID: 31810760)
RNA. 2018 Apr;24(4):499-512. (PMID: 29348140)
Nat Commun. 2019 Nov 12;10(1):5126. (PMID: 31719534)
Cell. 2015 Nov 5;163(4):999-1010. (PMID: 26593424)
Nucleic Acids Res. 1979 Jun 25;6(8):2879-99. (PMID: 223130)
Nucleic Acids Res. 2018 Jun 20;46(11):5776-5791. (PMID: 29373715)
RNA. 2018 Oct;24(10):1403-1417. (PMID: 30012570)
Nat Chem Biol. 2014 Feb;10(2):93-5. (PMID: 24316715)
Cell Host Microbe. 2016 Nov 9;20(5):666-673. (PMID: 27773536)
Mol Cell. 2020 May 21;78(4):624-640.e7. (PMID: 32380061)
Sci Rep. 2018 Sep 14;8(1):13827. (PMID: 30218090)
Genome Biol. 2008;9(9):R137. (PMID: 18798982)
Nat Chem Biol. 2011 Oct 16;7(12):885-7. (PMID: 22002720)
J Biol Chem. 1994 Jul 1;269(26):17697-704. (PMID: 8021282)
J Virol. 2022 Nov 23;96(22):e0099722. (PMID: 36314819)
Mol Cell. 2010 May 28;38(4):576-89. (PMID: 20513432)
Angew Chem Int Ed Engl. 2015 Jan 26;54(5):1587-90. (PMID: 25491922)
Elife. 2019 Oct 24;8:. (PMID: 31647415)
Bioinformatics. 2013 Jan 1;29(1):15-21. (PMID: 23104886)
Cell Res. 2017 Mar;27(3):315-328. (PMID: 28106072)
Nature. 2012 Apr 29;485(7397):201-6. (PMID: 22575960)
RNA. 2023 Jun;29(6):756-763. (PMID: 36889928)
J Virol. 1976 Oct;20(1):45-53. (PMID: 1086370)
PLoS Pathog. 2018 Feb 15;14(2):e1006919. (PMID: 29447282)
Mol Cell. 2013 Jan 10;49(1):18-29. (PMID: 23177736)
Cell. 2012 Jun 22;149(7):1635-46. (PMID: 22608085)
RNA. 2022 Nov;28(11):1430-1439. (PMID: 36104106)
Nature. 2016 May 25;534(7608):575-8. (PMID: 27281194)
RNA Biol. 2021 Oct 15;18(sup1):31-40. (PMID: 34559589)
Nat Commun. 2022 Aug 11;13(1):4725. (PMID: 35953468)
Nat Commun. 2019 Sep 9;10(1):4079. (PMID: 31501426)
Viruses. 2021 Jun 01;13(6):. (PMID: 34205979)
Bioinformatics. 2018 Sep 15;34(18):3094-3100. (PMID: 29750242)
Mol Cell. 2018 Sep 20;71(6):973-985.e5. (PMID: 30197295)
Methods Mol Biol. 2023;2624:185-205. (PMID: 36723817)
PLoS One. 2011 Jan 14;6(1):e16210. (PMID: 21264265)
Nucleic Acids Res. 1976 Mar;3(3):749-65. (PMID: 1272797)
Nature. 2014 Jan 2;505(7481):117-20. (PMID: 24284625)
J Virol. 2006 May;80(10):5059-64. (PMID: 16641297)
المشرفين على المادة: 0 (RNA, Viral)
CLE6G00625 (N-methyladenosine)
0 (Antibodies, Viral)
K72T3FS567 (Adenosine)
تواريخ الأحداث: Date Created: 20240312 Date Completed: 20240313 Latest Revision: 20240315
رمز التحديث: 20240315
مُعرف محوري في PubMed: PMC10928186
DOI: 10.1038/s41467-024-46278-9
PMID: 38467633
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-024-46278-9