دورية أكاديمية
[Characterization of Reductive Dechlorination of Chlorinated Ethylenes by Anaerobic Consortium].
| العنوان: | [Characterization of Reductive Dechlorination of Chlorinated Ethylenes by Anaerobic Consortium]. |
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| المؤلفون: | Li W; Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China., Liu GP; Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China., Liu J; Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China., Lü LH; Jiangsu Provincial Key Laboratory of Environmental Engineering, Jiangsu Provincial Academy of Environmental Science, Nanjing 210036, China., Qiao WJ; Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China., Yu X; Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China., Zhang XY; Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China., Jiang JD; Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. |
| المصدر: | Huan jing ke xue= Huanjing kexue [Huan Jing Ke Xue] 2024 Feb 08; Vol. 45 (2), pp. 1080-1089. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Ke xue zhu ban she Country of Publication: China NLM ID: 8405344 Publication Model: Print Cited Medium: Print ISSN: 0250-3301 (Print) Linking ISSN: 02503301 NLM ISO Abbreviation: Huan Jing Ke Xue Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Beijing : Ke xue zhu ban she, |
| مواضيع طبية MeSH: | Trichloroethylene* , Tetrachloroethylene* , Chloroflexi*/genetics , Ethylene Dichlorides*, Humans ; Anaerobiosis ; RNA, Ribosomal, 16S/genetics ; Ethylenes ; Dichloroethylenes ; Biodegradation, Environmental |
| مستخلص: | Tetrachloroethylene （PCE） and trichloroethylene （TCE） are typical volatile halogenated organic compounds in groundwater that pose serious threats to the ecological environment and human health. To obtain an anaerobic microbial consortium capable of efficiently dechlorinating PCE and TCE to a non-toxic end product and to explore its potential in treating contaminated groundwater， an anaerobic microbial consortium W-1 that completely dechlorinated PCE and TCE to ethylene was obtained by repeatedly feeding PCE or TCE into the contaminated groundwater collected from an industrial site. The dechlorination rates of PCE and TCE were （120.1 ±4.9） μmol·（L·d） -1 and （172.4 ±21.8） μmol·（L·d） -1 in W-1， respectively. 16S rRNA gene amplicon sequencing and quantitative PCR （qPCR） showed that the relative abundance of Dehalobacter increased from 1.9% to 57.1%， with the gene copy number increasing by 1.7×10 7 copies per 1 μmol Cl - released when 98.3 μmol of PCE was dechlorinated to cis -1，2-dichloroethylene （ cis -1，2-DCE）. The relative abundance of Dehalococcoides increased from 1.1% to 53.8% when cis -1，2-DCE was reductively dechlorinated to ethylene. The growth yield of Dehalococcoides gene copy number increased by 1.7×10 8 copies per 1 μmol Cl - released for the complete reductive dechlorination of PCE to ethylene. The results indicated that Dehalobacter and Dehalococcoides cooperated to completely detoxify PCE. When TCE was used as the only electron acceptor， the relative abundance of Dehalococcoides increased from （29.1 ±2.4）% to （7.7 ±0.2）%， and gene copy number increased by （1.9 ±0.4）×10 8 copies per 1 μmol Cl - released， after dechlorinating 222.8 μmol of TCE to ethylene. The 16S rRNA gene sequence of Dehalococcoides LWT1， the main functional dehalogenating bacterium in enrichment culture W-1， was obtained using PCR and Sanger sequencing， and it showed 100% similarity with the 16S rRNA gene sequence of D. mccartyi strain 195. The anaerobic microbial consortium W-1 was also bioaugmented into the groundwater contaminated by TCE at a concentration of 418.7 μmol·L -1 . The results showed that （69.2 ±9.8）% of TCE could be completely detoxified to ethylene within 28 days with a dechlorination rate of （10.3 ±1.5） μmol·（L·d） -1 . This study can provide the microbial resource and theoretical guidance for the anaerobic microbial remediation in PCE or TCE-contaminated groundwater. |
| فهرسة مساهمة: | Keywords: Dehalococcoides; anaerobic microbial reductive dechlorination; organohalide-respiring bacteria; tetrachloroethene（PCE）; trichloroethylene（TCE） |
| المشرفين على المادة: | 0 (RNA, Ribosomal, 16S) 91GW059KN7 (ethylene) 0 (Ethylenes) 0 (Dichloroethylenes) 290YE8AR51 (Trichloroethylene) TJ904HH8SN (Tetrachloroethylene) 55163IJI47 (ethylene dichloride) 0 (Ethylene Dichlorides) |
| تواريخ الأحداث: | Date Created: 20240312 Date Completed: 20240314 Latest Revision: 20240314 |
| رمز التحديث: | 20240314 |
| DOI: | 10.13227/j.hjkx.202302134 |
| PMID: | 38471945 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0250-3301 |
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| DOI: | 10.13227/j.hjkx.202302134 |