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Hypolipidemic Effect of Rice Bran Oil Extract Tocotrienol in High-Fat Diet-Induced Hyperlipidemia Zebrafish (Danio Rerio) Induced by High-Fat Diet.

التفاصيل البيبلوغرافية
العنوان: Hypolipidemic Effect of Rice Bran Oil Extract Tocotrienol in High-Fat Diet-Induced Hyperlipidemia Zebrafish (Danio Rerio) Induced by High-Fat Diet.
المؤلفون: Liu N; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China., Zhang P; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China., Xue M; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China., Zhang M; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China., Huang Z; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China., Xu C; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China., Meng Y; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China., Fan Y; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China., Liu W; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China., Zhang F; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.; College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China., Chen P; Institute of Fishery Research of Xinjiang Uygur Autonenous Region, Urumqi 830099, China., Zhou Y; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2024 Mar 03; Vol. 25 (5). Date of Electronic Publication: 2024 Mar 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Tocotrienols*/metabolism , Hyperlipidemias*/metabolism , Diabetes Mellitus, Type 2*/metabolism, Humans ; Mice ; Rats ; Animals ; Zebrafish/metabolism ; Diet, High-Fat ; Rice Bran Oil ; PPAR gamma/metabolism ; RNA, Messenger/metabolism ; Lipid Metabolism ; Liver/metabolism
مستخلص: In recent years, the potent influence of tocotrienol (T3) on diminishing blood glucose and lipid concentrations in both Mus musculus (rats) and Homo sapiens (humans) has been established. However, the comprehensive exploration of tocotrienol's hypolipidemic impact and the corresponding mechanisms in aquatic species remains inadequate. In this study, we established a zebrafish model of a type 2 diabetes mellitus (T2DM) model through high-fat diet administration to zebrafish. In the T2DM zebrafish, the thickness of ocular vascular walls significantly increased compared to the control group, which was mitigated after treatment with T3. Additionally, our findings demonstrate the regulatory effect of T3 on lipid metabolism, leading to the reduced synthesis and storage of adipose tissue in zebrafish. We validated the expression patterns of genes relevant to these processes using RT-qPCR. In the T2DM model, there was an almost two-fold upregulation in pparγ and cyp7a1 mRNA levels, coupled with a significant downregulation in cpt1a mRNA ( p < 0.01) compared to the control group. The ELISA revealed that the protein expression levels of Pparγ and Rxrα exhibited a two-fold elevation in the T2DM group relative to the control. In the T3-treated group, Pparγ and Rxrα protein expression levels consistently exhibited a two-fold decrease compared to the model group. Lipid metabolomics showed that T3 could affect the metabolic pathways of zebrafish lipid regulation, including lipid synthesis and decomposition. We provided experimental evidence that T3 could mitigate lipid accumulation in our zebrafish T2DM model. Elucidating the lipid-lowering effects of T3 could help to minimize the detrimental impacts of overfeeding in aquaculture.
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معلومات مُعتمدة: 2022BBA0054, 2023BBB122 CARS-45, technical innovation special project of Hubei Province; 32202980 the national natural science foundation of China; 2023TD46, YFI 202207 central public-interest scientific institution basal research fund
فهرسة مساهمة: Keywords: blood fat; high-fat diet; rice bran oil extract; tocotrienol (T3); type 2 diabetes mellitus (T2DM) model; zebrafish
المشرفين على المادة: 0 (Tocotrienols)
LZO6K1506A (Rice Bran Oil)
0 (PPAR gamma)
0 (RNA, Messenger)
تواريخ الأحداث: Date Created: 20240313 Date Completed: 20240314 Latest Revision: 20240315
رمز التحديث: 20240315
مُعرف محوري في PubMed: PMC10931685
DOI: 10.3390/ijms25052954
PMID: 38474201
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms25052954