دورية أكاديمية
Hypolipidemic Effect of Rice Bran Oil Extract Tocotrienol in High-Fat Diet-Induced Hyperlipidemia Zebrafish (Danio Rerio) Induced by High-Fat Diet.
العنوان: | Hypolipidemic Effect of Rice Bran Oil Extract Tocotrienol in High-Fat Diet-Induced Hyperlipidemia Zebrafish (Danio Rerio) Induced by High-Fat Diet. |
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المؤلفون: | Liu N; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China., Zhang P; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China., Xue M; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China., Zhang M; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China., Huang Z; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China., Xu C; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China., Meng Y; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China., Fan Y; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China., Liu W; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China., Zhang F; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.; College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China., Chen P; Institute of Fishery Research of Xinjiang Uygur Autonenous Region, Urumqi 830099, China., Zhou Y; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China. |
المصدر: | International journal of molecular sciences [Int J Mol Sci] 2024 Mar 03; Vol. 25 (5). Date of Electronic Publication: 2024 Mar 03. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2000- |
مواضيع طبية MeSH: | Tocotrienols*/metabolism , Hyperlipidemias*/metabolism , Diabetes Mellitus, Type 2*/metabolism, Humans ; Mice ; Rats ; Animals ; Zebrafish/metabolism ; Diet, High-Fat ; Rice Bran Oil ; PPAR gamma/metabolism ; RNA, Messenger/metabolism ; Lipid Metabolism ; Liver/metabolism |
مستخلص: | In recent years, the potent influence of tocotrienol (T3) on diminishing blood glucose and lipid concentrations in both Mus musculus (rats) and Homo sapiens (humans) has been established. However, the comprehensive exploration of tocotrienol's hypolipidemic impact and the corresponding mechanisms in aquatic species remains inadequate. In this study, we established a zebrafish model of a type 2 diabetes mellitus (T2DM) model through high-fat diet administration to zebrafish. In the T2DM zebrafish, the thickness of ocular vascular walls significantly increased compared to the control group, which was mitigated after treatment with T3. Additionally, our findings demonstrate the regulatory effect of T3 on lipid metabolism, leading to the reduced synthesis and storage of adipose tissue in zebrafish. We validated the expression patterns of genes relevant to these processes using RT-qPCR. In the T2DM model, there was an almost two-fold upregulation in pparγ and cyp7a1 mRNA levels, coupled with a significant downregulation in cpt1a mRNA ( p < 0.01) compared to the control group. The ELISA revealed that the protein expression levels of Pparγ and Rxrα exhibited a two-fold elevation in the T2DM group relative to the control. In the T3-treated group, Pparγ and Rxrα protein expression levels consistently exhibited a two-fold decrease compared to the model group. Lipid metabolomics showed that T3 could affect the metabolic pathways of zebrafish lipid regulation, including lipid synthesis and decomposition. We provided experimental evidence that T3 could mitigate lipid accumulation in our zebrafish T2DM model. Elucidating the lipid-lowering effects of T3 could help to minimize the detrimental impacts of overfeeding in aquaculture. |
References: | Nutrients. 2017 Sep 14;9(9):. (PMID: 28906458) Nat Commun. 2020 Oct 14;11(1):5155. (PMID: 33056995) PLoS One. 2012;7(8):e43502. (PMID: 22912885) Dev Cell. 2012 Jun 12;22(6):1261-74. (PMID: 22579223) Cancer Biol Med. 2023 Jan 12;20(1):. (PMID: 36647790) Antioxidants (Basel). 2022 Mar 17;11(3):. (PMID: 35326228) Molecules. 2021 Apr 14;26(8):. (PMID: 33919764) Cell Death Differ. 2022 Jan;29(1):118-132. (PMID: 34413485) Antioxidants (Basel). 2023 Jan 03;12(1):. (PMID: 36670983) BMC Cancer. 2018 Jan 4;18(1):22. (PMID: 29301506) Am J Clin Nutr. 2020 Nov 11;112(5):1200-1211. (PMID: 32930325) Am J Pathol. 2007 May;170(5):1511-20. (PMID: 17456758) Antimicrob Agents Chemother. 2022 Jan 18;66(1):e0109821. (PMID: 34633849) Evid Based Complement Alternat Med. 2012;2012:740671. (PMID: 22956978) Mol Metab. 2019 Nov;29:99-113. (PMID: 31668396) Nutrients. 2019 Jul 04;11(7):. (PMID: 31277481) Sci Rep. 2017 Aug 8;7(1):7484. (PMID: 28790401) Molecules. 2022 Oct 21;27(20):. (PMID: 36296700) Public Health Rev. 2018 Aug 08;39:21. (PMID: 30094087) Mol Med Rep. 2012 Apr;5(4):905-9. (PMID: 22293775) Cells. 2020 May 28;9(6):. (PMID: 32481590) Sci Total Environ. 2019 Oct 15;687:1344-1356. (PMID: 31412468) Int J Mol Sci. 2022 Sep 15;23(18):. (PMID: 36142687) Fish Shellfish Immunol. 2023 May;136:108740. (PMID: 37061070) Dev Cell. 2012 Oct 16;23(4):691-704. (PMID: 23079596) BMC Dev Biol. 2007 Oct 15;7:114. (PMID: 17937808) Front Vet Sci. 2022 Feb 22;9:829338. (PMID: 35296058) Antioxidants (Basel). 2022 Jan 25;11(2):. (PMID: 35204111) Cell Mol Biol Lett. 2018 Apr 18;23:16. (PMID: 29721023) PLoS One. 2019 Dec 30;14(12):e0218360. (PMID: 31887112) Antioxidants (Basel). 2021 Oct 26;10(11):. (PMID: 34829560) Hum Mol Genet. 2017 May 1;26(9):1770-1784. (PMID: 28334899) J Clin Endocrinol Metab. 2018 Oct 1;103(10):3757-3766. (PMID: 30113648) Saudi J Biol Sci. 2023 Feb;30(2):103558. (PMID: 36712183) Molecules. 2017 Oct 25;22(11):. (PMID: 29068394) Front Chem. 2023 Jan 09;10:1101557. (PMID: 36700070) Int J Mol Sci. 2017 Mar 25;18(4):. (PMID: 28346357) Mar Drugs. 2015 Apr 30;13(5):2732-56. (PMID: 25942093) Int J Mol Sci. 2020 Nov 18;21(22):. (PMID: 33218175) Heliyon. 2023 Jan 24;9(2):e13215. (PMID: 36816288) |
معلومات مُعتمدة: | 2022BBA0054, 2023BBB122 CARS-45, technical innovation special project of Hubei Province; 32202980 the national natural science foundation of China; 2023TD46, YFI 202207 central public-interest scientific institution basal research fund |
فهرسة مساهمة: | Keywords: blood fat; high-fat diet; rice bran oil extract; tocotrienol (T3); type 2 diabetes mellitus (T2DM) model; zebrafish |
المشرفين على المادة: | 0 (Tocotrienols) LZO6K1506A (Rice Bran Oil) 0 (PPAR gamma) 0 (RNA, Messenger) |
تواريخ الأحداث: | Date Created: 20240313 Date Completed: 20240314 Latest Revision: 20240315 |
رمز التحديث: | 20240315 |
مُعرف محوري في PubMed: | PMC10931685 |
DOI: | 10.3390/ijms25052954 |
PMID: | 38474201 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1422-0067 |
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DOI: | 10.3390/ijms25052954 |