دورية أكاديمية
Intra-nanoparticle plasmonic nanogap based spatial-confinement SERS analysis of polypeptides.
| العنوان: | Intra-nanoparticle plasmonic nanogap based spatial-confinement SERS analysis of polypeptides. |
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| المؤلفون: | Li R; College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China., Hu Y; College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China., Sun X; College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China., Zhang Z; College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China., Chen K; College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China., Liu Q; College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. Electronic address: iliuqi@csu.edu.cn., Chen X; College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China; Xiangjiang Laboratory, Changsha 410205, China. Electronic address: xqchen@csu.edu.cn. |
| المصدر: | Talanta [Talanta] 2024 Jun 01; Vol. 273, pp. 125899. Date of Electronic Publication: 2024 Mar 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Oxford : Pergamon Press |
| مواضيع طبية MeSH: | Metal Nanoparticles*/chemistry , Nanostructures*/chemistry , Chlorides* , Gold Compounds*, Gold/chemistry ; Spectrum Analysis, Raman/methods |
| مستخلص: | Sensing and characterizing water-soluble polypeptides are essential in various biological applications. However, detecting polypeptides using Surface-Enhanced Raman Scattering (SERS) remains a challenge due to the dominance of aromatic amino acid residues and backbones in the signal, which hinders the detection of non-aromatic amino acid residues. Herein, intra-nanoparticle plasmonic nanogap were designed by etching the Ag shell in Au@AgNPs (i.e., obtaining AuAg cores) with chlorauric acid under mild conditions, at the same time forming the outermost Au shell and the void between the AuAg cores and the Au shell (AuAg@void@Au). By varying the Ag to added chloroauric acid molar ratios, we pioneered a simple, controllable, and general synthetic strategy to form interlayer-free nanoparticles with tunable Au shell thickness, achieving precise regulation of electric field enhancement within the intra-nanogap. As validation, two polypeptide molecules, bacitracin and insulin B, were successfully synchronously encapsulated and spatial-confined in the intra-nanogap for sensing. Compared with concentrated 50 nm AuNPs and Au@AgNPs as SERS substrates, our simultaneous detection method improved the sensitivity of the assay while benefiting to obtain more comprehensive characteristic peaks of polypeptides. The synthetic strategy of confining analytes while fabricating plasmonic nanostructures enables the diffusion of target molecules into the nanogap in a highly specific and sensitive manner, providing the majority of the functionality required to achieve peptide detection or sequencing without the hassle of labeling. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Electric field enhancement regulation; Intra-nanoparticle plasmonic nanogap; Polypeptides analysis; Surface-enhanced Raman scattering (SERS) |
| المشرفين على المادة: | 7440-57-5 (Gold) 8H372EGX3V (gold tetrachloride, acid) 0 (Chlorides) 0 (Gold Compounds) |
| تواريخ الأحداث: | Date Created: 20240314 Date Completed: 20240409 Latest Revision: 20240409 |
| رمز التحديث: | 20240409 |
| DOI: | 10.1016/j.talanta.2024.125899 |
| PMID: | 38484502 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3573 |
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| DOI: | 10.1016/j.talanta.2024.125899 |