دورية أكاديمية
Dietary n-3 polyunsaturated fatty acids alter the number, fatty acid profile and coagulatory activity of circulating and platelet-derived extracellular vesicles: a randomized, controlled crossover trial.
| العنوان: | Dietary n-3 polyunsaturated fatty acids alter the number, fatty acid profile and coagulatory activity of circulating and platelet-derived extracellular vesicles: a randomized, controlled crossover trial. |
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| المؤلفون: | Bozbas E; Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Reading, United Kingdom., Zhou R; Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Reading, United Kingdom., Soyama S; Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Reading, United Kingdom., Allen-Redpath K; Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Reading, United Kingdom., Mitchell JL; Institute for Cardiovascular and Metabolic Research and School of Biological Sciences, University of Reading, Reading, United Kingdom., Fisk HL; School of Human Development and Health, Faculty of Medicine, University of Southampton, Southampton, United Kingdom., Calder PC; School of Human Development and Health, Faculty of Medicine, University of Southampton, Southampton, United Kingdom; NIHR Southampton Biomedical Research Centre, University Hospital Southampton NHS Foundation Trust and University of Southampton, Southampton, United Kingdom., Jones C; Institute for Cardiovascular and Metabolic Research and School of Biological Sciences, University of Reading, Reading, United Kingdom., Gibbins JM; Institute for Cardiovascular and Metabolic Research and School of Biological Sciences, University of Reading, Reading, United Kingdom., Fischer R; Target Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom., Hester S; Target Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom., Yaqoob P; Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Reading, United Kingdom. Electronic address: p.yaqoob@reading.ac.uk. |
| المصدر: | The American journal of clinical nutrition [Am J Clin Nutr] 2024 May; Vol. 119 (5), pp. 1175-1186. Date of Electronic Publication: 2024 Mar 13. |
| نوع المنشور: | Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0376027 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1938-3207 (Electronic) Linking ISSN: 00029165 NLM ISO Abbreviation: Am J Clin Nutr Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : [New York, NY] : Elsevier Original Publication: Bethesda, MD : American Society of Clinical Nutrition |
| مواضيع طبية MeSH: | Extracellular Vesicles*/metabolism , Fatty Acids, Omega-3*/pharmacology , Cross-Over Studies* , Blood Coagulation*/drug effects , Blood Platelets*/metabolism , Blood Platelets*/drug effects, Humans ; Male ; Female ; Middle Aged ; Double-Blind Method ; Dietary Supplements ; Cardiovascular Diseases/prevention & control ; Adult ; Fish Oils/pharmacology ; Fish Oils/administration & dosage ; Aged ; Fatty Acids/metabolism |
| مستخلص: | Background: Extracellular vesicles (EVs) are proposed to play a role in the development of cardiovascular diseases (CVDs) and are considered emerging markers of CVDs. n-3 PUFAs are abundant in oily fish and fish oil and are reported to reduce CVD risk, but there has been little research to date examining the effects of n-3 PUFAs on the generation and function of EVs. Objectives: We aimed to investigate the effects of fish oil supplementation on the number, generation, and function of EVs in subjects with moderate risk of CVDs. Methods: A total of 40 participants with moderate risk of CVDs were supplemented with capsules containing either fish oil (1.9 g/d n-3 PUFAs) or control oil (high-oleic safflower oil) for 12 wk in a randomized, double-blind, placebo-controlled crossover intervention study. The effects of fish oil supplementation on conventional CVD and thrombogenic risk markers were measured, along with the number and fatty acid composition of circulating and platelet-derived EVs (PDEVs). PDEV proteome profiles were evaluated, and their impact on coagulation was assessed using assays including fibrin clot formation, thrombin generation, fibrinolysis, and ex vivo thrombus formation. Results: n-3 PUFAs decreased the numbers of circulating EVs by 27%, doubled their n-3 PUFA content, and reduced their capacity to support thrombin generation by >20% in subjects at moderate risk of CVDs. EVs derived from n-3 PUFA-enriched platelets in vitro also resulted in lower thrombin generation, but did not alter thrombus formation in a whole blood ex vivo assay. Conclusions: Dietary n-3 PUFAs alter the number, composition, and function of EVs, reducing their coagulatory activity. This study provides clear evidence that EVs support thrombin generation and that this EV-dependent thrombin generation is reduced by n-3 PUFAs, which has implications for prevention and treatment of thrombosis. Clinical Trial Registry: This trial was registered at clinicaltrials.gov as NCT03203512. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
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| فهرسة مساهمة: | Keywords: cardiovascular disease; coagulation; extracellular vesicles; fish oil; platelet-derived extracellular vesicles; thrombosis |
| سلسلة جزيئية: | ClinicalTrials.gov NCT03203512 |
| المشرفين على المادة: | 0 (Fatty Acids, Omega-3) 0 (Fish Oils) 0 (Fatty Acids) |
| تواريخ الأحداث: | Date Created: 20240314 Date Completed: 20240503 Latest Revision: 20240722 |
| رمز التحديث: | 20240723 |
| مُعرف محوري في PubMed: | PMC11130656 |
| DOI: | 10.1016/j.ajcnut.2024.03.008 |
| PMID: | 38484976 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1938-3207 |
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| DOI: | 10.1016/j.ajcnut.2024.03.008 |