دورية أكاديمية
Hybrid Molecular and Functional Micro-CT Imaging Reveals Increased Myocardial Apoptosis Preceding Cardiac Failure in Progeroid Ercc1 Mice.
| العنوان: | Hybrid Molecular and Functional Micro-CT Imaging Reveals Increased Myocardial Apoptosis Preceding Cardiac Failure in Progeroid Ercc1 Mice. |
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| المؤلفون: | van Thiel BS; Department of Molecular Genetics, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, The Netherlands.; Department of Vascular Surgery, Erasmus MC Cardiovascular Institute, Erasmus University Medical Center, Room 702A, Wytemaweg 80, 3015 CN, Rotterdam, The Netherlands.; Division of Vascular Medicine and Pharmacology, Department of Internal Medicine, Erasmus University Medical Center, Rotterdam, The Netherlands., de Boer M; Division of Experimental Cardiology, Department of Cardiology, Erasmus MC Cardiovascular Institute, Erasmus University Medical Center, Rotterdam, The Netherlands., Ridwan Y; Department of Molecular Genetics, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, The Netherlands.; Department of Radiotherapy, Erasmus University Medical Center, Room 702A, Wytemaweg 80, 3015 CN, Rotterdam, The Netherlands., de Kleijnen MGJ; Division of Experimental Cardiology, Department of Cardiology, Erasmus MC Cardiovascular Institute, Erasmus University Medical Center, Rotterdam, The Netherlands., van Vliet N; Department of Molecular Genetics, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, The Netherlands., van der Linden J; Department of Molecular Genetics, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, The Netherlands.; Division of Vascular Medicine and Pharmacology, Department of Internal Medicine, Erasmus University Medical Center, Rotterdam, The Netherlands.; Division of Experimental Cardiology, Department of Cardiology, Erasmus MC Cardiovascular Institute, Erasmus University Medical Center, Rotterdam, The Netherlands., de Beer I; Department of Molecular Genetics, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, The Netherlands., van Heijningen PM; Department of Molecular Genetics, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, The Netherlands., Vermeij WP; Department of Molecular Genetics, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, The Netherlands.; Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.; Oncode Institute, Utrecht, The Netherlands., Hoeijmakers JHJ; Department of Molecular Genetics, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, The Netherlands.; Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.; Oncode Institute, Utrecht, The Netherlands.; Institute for Genome Stability in Aging and Disease, Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany., Danser AHJ; Division of Vascular Medicine and Pharmacology, Department of Internal Medicine, Erasmus University Medical Center, Rotterdam, The Netherlands., Kanaar R; Department of Molecular Genetics, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, The Netherlands.; Oncode Institute, Utrecht, The Netherlands., Duncker DJ; Division of Experimental Cardiology, Department of Cardiology, Erasmus MC Cardiovascular Institute, Erasmus University Medical Center, Rotterdam, The Netherlands., van der Pluijm I; Department of Molecular Genetics, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, The Netherlands. i.vanderpluijm@erasmusmc.nl.; Department of Vascular Surgery, Erasmus MC Cardiovascular Institute, Erasmus University Medical Center, Room 702A, Wytemaweg 80, 3015 CN, Rotterdam, The Netherlands. i.vanderpluijm@erasmusmc.nl., Essers J; Department of Molecular Genetics, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, The Netherlands. j.essers@erasmusmc.nl.; Department of Vascular Surgery, Erasmus MC Cardiovascular Institute, Erasmus University Medical Center, Room 702A, Wytemaweg 80, 3015 CN, Rotterdam, The Netherlands. j.essers@erasmusmc.nl.; Department of Radiotherapy, Erasmus University Medical Center, Room 702A, Wytemaweg 80, 3015 CN, Rotterdam, The Netherlands. j.essers@erasmusmc.nl. |
| المصدر: | Molecular imaging and biology [Mol Imaging Biol] 2024 Aug; Vol. 26 (4), pp. 628-637. Date of Electronic Publication: 2024 Mar 18. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: United States NLM ID: 101125610 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1860-2002 (Electronic) Linking ISSN: 15361632 NLM ISO Abbreviation: Mol Imaging Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2005- : New York, NY : Springer Original Publication: New York, NY : Elsevier Science, c2001- |
| مواضيع طبية MeSH: | Apoptosis* , Heart Failure*/diagnostic imaging , Heart Failure*/pathology , DNA-Binding Proteins*/metabolism , DNA-Binding Proteins*/genetics , X-Ray Microtomography* , Endonucleases*/metabolism , Endonucleases*/genetics , Myocardium*/pathology, Animals ; Mice |
| مستخلص: | Purpose: In this study, we explored the role of apoptosis as a potential biomarker for cardiac failure using functional micro-CT and fluorescence molecular tomography (FMT) imaging techniques in Ercc1 mutant mice. Ercc1 is involved in multiple DNA repair pathways, and its mutations contribute to accelerated aging phenotypes in both humans and mice, due to the accumulation of DNA lesions that impair vital DNA functions. We previously found that systemic mutations and cardiomyocyte-restricted deletion of Ercc1 in mice results in left ventricular (LV) dysfunction at older age. Procedures and Results: Here we report that combined functional micro-CT and FMT imaging allowed us to detect apoptosis in systemic Ercc1 mutant mice prior to the development of overt LV dysfunction, suggesting its potential as an early indicator and contributing factor of cardiac impairment. The detection of apoptosis in vivo was feasible as early as 12 weeks of age, even when global LV function appeared normal, underscoring the potential of apoptosis as an early predictor of LV dysfunction, which subsequently manifested at 24 weeks. Conclusions: This study highlights the utility of combined functional micro-CT and FMT imaging in assessing cardiac function and detecting apoptosis, providing valuable insights into the potential of apoptosis as an early biomarker for cardiac failure. (© 2024. The Author(s).) |
| References: | Nat Rev Cardiol. 2011 Jan;8(1):30-41. (PMID: 21060326) Science. 2002 May 17;296(5571):1276-9. (PMID: 11950998) Invest Radiol. 2007 Feb;42(2):85-94. (PMID: 17220726) Nat Commun. 2017 Apr 24;8:15104. (PMID: 28436431) Circulation. 2002 Dec 10;106(24):3068-72. (PMID: 12473553) Mol Imaging. 2005 Apr-Jun;4(2):110-6. (PMID: 16105509) J Am Coll Cardiol. 2002 Sep 18;40(6):1097-103; discussion 1104-5. (PMID: 12354434) Eur J Clin Invest. 1999 May;29(5):380-6. (PMID: 10354194) Curr Biol. 1997 Jun 1;7(6):427-39. (PMID: 9197240) Nature. 2016 Sep 15;537(7620):427-431. (PMID: 27556946) Int J Cardiol. 2013 Sep 10;167(6):2373-86. (PMID: 23498286) Annu Rev Pharmacol Toxicol. 2016;56:427-45. (PMID: 26514200) J Vis Exp. 2016 Feb 16;(108):53603. (PMID: 26967592) Aging Cell. 2023 Mar;22(3):e13768. (PMID: 36756698) EMBO J. 2001 Nov 15;20(22):6540-9. (PMID: 11707424) N Engl J Med. 1997 Apr 17;336(16):1131-41. (PMID: 9099657) Cell. 2023 Jan 19;186(2):243-278. (PMID: 36599349) Nature. 2021 Apr;592(7856):695-703. (PMID: 33911272) J Pathol. 2003 Feb;199(2):229-36. (PMID: 12533836) Can J Cardiol. 2010 Mar;26 Suppl A:13A-16A. (PMID: 20386754) Mutat Res. 2007 Aug 1;621(1-2):5-17. (PMID: 17383689) Circulation. 1999 Jun 1;99(21):2757-64. (PMID: 10351969) Am J Epidemiol. 1996 May 1;143(9):881-8. (PMID: 8610701) J Clin Invest. 1997 Jul 1;100(1):169-79. (PMID: 9202069) Aging Cell. 2023 Apr;22(4):e13782. (PMID: 36734200) Cardiovasc Res. 2021 Feb 22;117(3):663-673. (PMID: 32170926) Nat Genet. 2023 Feb;55(2):268-279. (PMID: 36658433) |
| فهرسة مساهمة: | Keywords: Aging; CT; DNA repair; Ercc1; FMT; Heart failure; Molecular imaging |
| المشرفين على المادة: | 0 (DNA-Binding Proteins) EC 3.1.- (Endonucleases) EC 3.1.- (Ercc1 protein, mouse) |
| تواريخ الأحداث: | Date Created: 20240318 Date Completed: 20240727 Latest Revision: 20240903 |
| رمز التحديث: | 20240903 |
| مُعرف محوري في PubMed: | PMC11281969 |
| DOI: | 10.1007/s11307-024-01902-4 |
| PMID: | 38498063 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1860-2002 |
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| DOI: | 10.1007/s11307-024-01902-4 |