دورية أكاديمية
أعرض في EDS

Biochemical characterization of the meiosis-essential yet evolutionarily divergent topoisomerase VIB-like protein MTOPVIB from Arabidopsis thaliana.

التفاصيل البيبلوغرافية
العنوان: Biochemical characterization of the meiosis-essential yet evolutionarily divergent topoisomerase VIB-like protein MTOPVIB from Arabidopsis thaliana.
المؤلفون: Chen HW; Institute of Biochemical Sciences, National Taiwan University, 10617 Taipei, Taiwan., Yeh HY; Institute of Biochemical Sciences, National Taiwan University, 10617 Taipei, Taiwan., Chang CC; Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, 100233 Taipei, Taiwan., Kuo WC; Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, 100233 Taipei, Taiwan., Lin SW; Institute of Biological Chemistry, Academia Sinica, 11529 Taipei, Taiwan., Vrielynck N; Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), 78000,Versailles, France., Grelon M; Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), 78000,Versailles, France., Chan NL; Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, 100233 Taipei, Taiwan., Chi P; Institute of Biochemical Sciences, National Taiwan University, 10617 Taipei, Taiwan.; Institute of Biological Chemistry, Academia Sinica, 11529 Taipei, Taiwan.
المصدر: Nucleic acids research [Nucleic Acids Res] 2024 May 08; Vol. 52 (8), pp. 4541-4555.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.
مواضيع طبية MeSH: Arabidopsis*/genetics , Arabidopsis*/enzymology , Arabidopsis*/metabolism , Arabidopsis Proteins*/metabolism , Arabidopsis Proteins*/genetics , DNA Topoisomerases, Type II*/metabolism, Adenosine Triphosphate/metabolism ; Archaeal Proteins ; DNA Breaks, Double-Stranded ; DNA Topoisomerases/metabolism ; DNA Topoisomerases/genetics ; DNA-Binding Proteins/metabolism ; DNA-Binding Proteins/genetics ; Endodeoxyribonucleases/metabolism ; Endodeoxyribonucleases/genetics ; Endodeoxyribonucleases/chemistry ; Evolution, Molecular ; Meiosis ; Protein Multimerization
مستخلص: Formation of programmed DNA double-strand breaks is essential for initiating meiotic recombination. Genetic studies on Arabidopsis thaliana and Mus musculus have revealed that assembly of a type IIB topoisomerase VI (Topo VI)-like complex, composed of SPO11 and MTOPVIB, is a prerequisite for generating DNA breaks. However, it remains enigmatic if MTOPVIB resembles its Topo VI subunit B (VIB) ortholog in possessing robust ATPase activity, ability to undergo ATP-dependent dimerization, and activation of SPO11-mediated DNA cleavage. Here, we successfully prepared highly pure A. thaliana MTOPVIB and MTOPVIB-SPO11 complex. Contrary to expectations, our findings highlight that MTOPVIB differs from orthologous Topo VIB by lacking ATP-binding activity and independently forming dimers without ATP. Most significantly, our study reveals that while MTOPVIB lacks the capability to stimulate SPO11-mediated DNA cleavage, it functions as a bona fide DNA-binding protein and plays a substantial role in facilitating the dsDNA binding capacity of the MOTOVIB-SPO11 complex. Thus, we illustrate mechanistic divergence between the MTOPVIB-SPO11 complex and classical type IIB topoisomerases.
(© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.)
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معلومات مُعتمدة: Academia Sinica; NTU-112L891605 National Taiwan University; MOST-107-2923-B-002-001-MY4 National Science and Technology Council; ANR ESSPOIR-17-CE12-0032 Agence Nationale de la Recherche; ANR-17-EUR-0007 Saclay Plant Sciences-SPS
المشرفين على المادة: 8L70Q75FXE (Adenosine Triphosphate)
0 (Arabidopsis Proteins)
0 (Archaeal Proteins)
EC 5.99.1.- (DNA topoisomerase VI)
EC 5.99.1.- (DNA Topoisomerases)
EC 5.99.1.3 (DNA Topoisomerases, Type II)
0 (DNA-Binding Proteins)
EC 3.1.- (Endodeoxyribonucleases)
EC 3.1.- (meiotic recombination protein SPO11)
تواريخ الأحداث: Date Created: 20240318 Date Completed: 20240508 Latest Revision: 20240530
رمز التحديث: 20240531
مُعرف محوري في PubMed: PMC11077084
DOI: 10.1093/nar/gkae181
PMID: 38499490
قاعدة البيانات: MEDLINE
الوصف
تدمد:1362-4962
DOI:10.1093/nar/gkae181