دورية أكاديمية
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Whole genome identification, molecular docking and expression analysis of enzymes involved in the selenomethionine cycle in Cardamine hupingshanensis.

التفاصيل البيبلوغرافية
العنوان: Whole genome identification, molecular docking and expression analysis of enzymes involved in the selenomethionine cycle in Cardamine hupingshanensis.
المؤلفون: Zeng X; Hubei Key Laboratory of Biological Resources Protection and Utilization, Enshi, China, Enshi.; Hubei Key Laboratory of Selenium Resource Research and Biological Application, Enshi, China, 44500.; College of Forestry and Horticulture, Hubei Minzu University, Enshi, China, 44500., Luo G; College of Biological and Food Engineering, Hubei Minzu University, Enshi, China, 44500., Fan Z; College of Biological and Food Engineering, Hubei Minzu University, Enshi, China, 44500., Xiao Z; Hubei Key Laboratory of Biological Resources Protection and Utilization, Enshi, China, Enshi.; College of Biological and Food Engineering, Hubei Minzu University, Enshi, China, 44500., Lu Y; Hubei Key Laboratory of Biological Resources Protection and Utilization, Enshi, China, Enshi., Xiao Q; College of Forestry and Horticulture, Hubei Minzu University, Enshi, China, 44500., Hou Z; College of Biological and Food Engineering, Hubei Minzu University, Enshi, China, 44500., Tang Q; Hubei Key Laboratory of Biological Resources Protection and Utilization, Enshi, China, Enshi. 330375856@qq.com.; College of Forestry and Horticulture, Hubei Minzu University, Enshi, China, 44500. 330375856@qq.com.; Hubei Engineering Research Center of Selenium Food Nutrition and Health Intelligent Technology, Enshi, China, 44500. 330375856@qq.com., Zhou Y; Hubei Key Laboratory of Selenium Resource Research and Biological Application, Enshi, China, 44500. 77416757@qq.com.; College of Biological and Food Engineering, Hubei Minzu University, Enshi, China, 44500. 77416757@qq.com.
المصدر: BMC plant biology [BMC Plant Biol] 2024 Mar 19; Vol. 24 (1), pp. 199. Date of Electronic Publication: 2024 Mar 19.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100967807 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2229 (Electronic) Linking ISSN: 14712229 NLM ISO Abbreviation: BMC Plant Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-
مواضيع طبية MeSH: Cardamine* , Selenium*, Selenomethionine ; 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase ; Molecular Docking Simulation ; Amino Acid Sequence ; Phylogeny ; Proteins
مستخلص: Background: The selenomethionine cycle (SeMTC) is a crucial pathway for the metabolism of selenium. The basic bioinformatics and functions of four enzymes involved in the cycle including S-adenosyl-methionine synthase (MAT), SAM-dependent methyltransferase (MTase), S-adenosyl-homocysteine hydrolase (SAHH) and methionine synthase (MTR), have been extensively reported in many eukaryotes. The identification and functional analyses of SeMTC genes/proteins in Cardamine hupingshanensis and their response to selenium stress have not yet been reported.
Results: In this study, 45 genes involved in SeMTC were identified in the C. hupingshanensis genome. Phylogenetic analysis showed that seven genes from ChMAT were clustered into four branches, twenty-seven genes from ChCOMT were clustered into two branches, four genes from ChSAHH were clustered into two branches, and seven genes from ChMTR were clustered into three branches. These genes were resided on 16 chromosomes. Gene structure and homologous protein modeling analysis illustrated that proteins in the same family are relatively conserved and have similar functions. Molecular docking showed that the affinity of SeMTC enzymes for selenium metabolites was higher than that for sulfur metabolites. The key active site residues identified for ChMAT were Ala 269 and Lys 273 , while Leu 221/231 and Gly 207/249 were determined as the crucial residues for ChCOMT. For ChSAHH, the essential active site residues were found to be Asn 87 , Asp 139 and Thr 206/207/208/325 . Ile 204 , Ser 111/329/377 , Asp 70/206/254 , and His 329/332/380 were identified as the critical active site residues for ChMTR. In addition, the results of the expression levels of four enzymes under selenium stress revealed that ChMAT3-1 genes were upregulated approximately 18-fold, ChCOMT9-1 was upregulated approximately 38.7-fold, ChSAHH1-2 was upregulated approximately 11.6-fold, and ChMTR3-2 genes were upregulated approximately 28-fold. These verified that SeMTC enzymes were involved in response to selenium stress to varying degrees.
Conclusions: The results of this research are instrumental for further functional investigation of SeMTC in C. hupingshanensis. This also lays a solid foundation for deeper investigations into the physiological and biochemical mechanisms underlying selenium metabolism in plants.
(© 2024. The Author(s).)
References: Cells. 2020 Jun 23;9(6):. (PMID: 32585935)
J Biol Chem. 2004 May 21;279(21):22548-57. (PMID: 15024005)
BMC Plant Biol. 2022 Oct 18;22(1):491. (PMID: 36253724)
Int J Mol Sci. 2022 Jul 31;23(15):. (PMID: 35955626)
Mol Plant. 2020 Aug 3;13(8):1194-1202. (PMID: 32585190)
J Mol Graph Model. 2004 Sep;23(1):77-87. (PMID: 15331056)
J Pineal Res. 2014 Sep;57(2):219-27. (PMID: 25039887)
Nat Prod Bioprospect. 2022 Aug 5;12(1):28. (PMID: 35927534)
Plant Mol Biol. 1997 Dec;35(6):981-6. (PMID: 9426618)
Plant Physiol. 2012 Aug;159(4):1834-44. (PMID: 22645068)
PLoS Biol. 2005 Feb;3(2):e31. (PMID: 15630480)
J Exp Bot. 2020 Jan 1;71(1):178-187. (PMID: 31563952)
Genome. 2007 Nov;50(11):1001-13. (PMID: 18059546)
Curr Opin Plant Biol. 2019 Aug;50:67-75. (PMID: 30959442)
J Chem Inf Model. 2021 Aug 23;61(8):3891-3898. (PMID: 34278794)
Int J Biol Macromol. 2020 May 15;151:554-565. (PMID: 32057875)
Biochem J. 2013 Apr 15;451(2):145-54. (PMID: 23535167)
J Biol Chem. 2004 Oct 22;279(43):44235-8. (PMID: 15326182)
Physiol Plant. 2002 Oct;116(2):238-247. (PMID: 12354201)
Plant Physiol. 2003 Mar;131(3):1460-7. (PMID: 12644695)
Plant Biol (Stuttg). 2012 Jan;14(1):1-10. (PMID: 22132825)
Nucleic Acids Res. 2019 Jul 2;47(W1):W345-W349. (PMID: 31114880)
Biochim Biophys Acta Gen Subj. 2018 Nov;1862(11):2333-2342. (PMID: 29751098)
J Appl Genet. 2009;50(3):213-23. (PMID: 19638676)
Annu Rev Plant Physiol Plant Mol Biol. 2000 Jun;51:401-432. (PMID: 15012198)
Antioxidants (Basel). 2021 May 31;10(6):. (PMID: 34072794)
EMBO J. 1994 Apr 15;13(8):1806-16. (PMID: 8168479)
Gene. 1989 Dec 14;84(2):359-69. (PMID: 2482229)
Plant Biotechnol J. 2018 Feb 7;:. (PMID: 29412503)
Nucleic Acids Res. 2003 Jul 1;31(13):3784-8. (PMID: 12824418)
Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):6117-21. (PMID: 11607550)
Metallomics. 2020 Apr 1;12(4):514-527. (PMID: 32055807)
Sci Rep. 2018 Feb 12;8(1):2789. (PMID: 29434336)
PeerJ. 2022 Mar 16;10:e13086. (PMID: 35313526)
Acta Crystallogr D Biol Crystallogr. 2012 Mar;68(Pt 3):218-31. (PMID: 22349223)
Biol Chem. 2000 Aug;381(8):695-703. (PMID: 11030427)
Planta. 2008 Jun;228(1):125-36. (PMID: 18350315)
Ann Bot. 2007 Jul;100(1):111-8. (PMID: 17525099)
Plant Physiol. 2016 Sep;172(1):244-53. (PMID: 27482079)
Plants (Basel). 2022 Mar 03;11(5):. (PMID: 35270160)
Plant Mol Biol. 1998 Jan;36(1):1-10. (PMID: 9484457)
J Biol Chem. 2003 Jan 24;278(4):2645-53. (PMID: 12438316)
Phytochemistry. 2006 Aug;67(15):1686-98. (PMID: 16766004)
Mol Biol Evol. 2021 Jun 25;38(7):3022-3027. (PMID: 33892491)
Methods. 2001 Dec;25(4):402-8. (PMID: 11846609)
Plant Physiol. 2010 Aug;153(4):1630-52. (PMID: 20498337)
New Phytol. 2017 Mar;213(4):1582-1596. (PMID: 27991670)
Arch Biochem Biophys. 2003 Jul 15;415(2):164-71. (PMID: 12831838)
Plant Signal Behav. 2019;14(8):1625697. (PMID: 31156026)
Biochim Biophys Acta Gen Subj. 2018 Nov;1862(11):2343-2353. (PMID: 29626605)
Front Nutr. 2021 May 28;8:685517. (PMID: 34124127)
J Theor Biol. 2004 Jan 7;226(1):33-43. (PMID: 14637052)
Nucleic Acids Res. 2015 Jul 1;43(W1):W443-7. (PMID: 25873628)
PLoS One. 2020 Jul 17;15(7):e0227466. (PMID: 32678822)
Cell Discov. 2021 Aug 10;7(1):62. (PMID: 34373445)
Nucleic Acids Res. 2014 Jul;42(Web Server issue):W320-4. (PMID: 24753421)
Plants (Basel). 2021 Dec 20;10(12):. (PMID: 34961287)
معلومات مُعتمدة: B2020088 Scientific Research project of Hubei Provincial Education Department; PT10202301 Open Foundation of Hubei Key Laboratory of Selenium Resource Research and Biological Application; 32260070 National Natural Science Foundation of China; T2020020 Excellent Young and Middle-aged Scientific and Technological Innovation Team Projects of Colleges and Universities in Hubei Province
فهرسة مساهمة: Keywords: Cardamine Hupingshanensis; Gene expression; Molecular docking; Selenium stress response; Selenomethionine cycle
المشرفين على المادة: 964MRK2PEL (Selenomethionine)
EC 2.1.1.13 (5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase)
H6241UJ22B (Selenium)
0 (Proteins)
تواريخ الأحداث: Date Created: 20240319 Date Completed: 20240320 Latest Revision: 20240321
رمز التحديث: 20240321
مُعرف محوري في PubMed: PMC10949594
DOI: 10.1186/s12870-024-04898-9
PMID: 38500044
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2229
DOI:10.1186/s12870-024-04898-9