دورية أكاديمية
Cholesterol depletion induces mesenchymal properties in oral squamous cell carcinoma cell line.
| العنوان: | Cholesterol depletion induces mesenchymal properties in oral squamous cell carcinoma cell line. |
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| المؤلفون: | do Nascimento RB; Postgraduate Program in Dentistry and Health, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil., Cerqueira PSG; Postgraduate Program in Dentistry and Health, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil., Silva JC; Postgraduate Program in Dentistry and Health, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil., Fontes EK; Postgraduate Program in Dentistry and Health, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil., Dos Santos EA; Postgraduate Program in Dentistry and Health, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil., Dos Santos JN; Laboratory of Oral and Maxillofacial Pathology, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil., Nunes FD; Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil., Rodrigues MFSD; Postgraduate Program in Biophotonics Applied to Health Sciences, Nove De Julho University (UNINOVE), São Paulo, São Paulo, Brazil., Paiva KBS; Laboratory of Extracellular Matrix Biology and Cellular Interaction, Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, São Paulo, São Paulo, Brazil., Xavier FCA; Laboratory of Oral and Maxillofacial Pathology, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil. |
| المصدر: | Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology [J Oral Pathol Med] 2024 Apr; Vol. 53 (4), pp. 246-257. Date of Electronic Publication: 2024 Mar 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Blackwell Country of Publication: Denmark NLM ID: 8911934 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-0714 (Electronic) Linking ISSN: 09042512 NLM ISO Abbreviation: J Oral Pathol Med Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford, UK : Wiley-Blackwell Original Publication: Copenhagen : Munksgaard, c1989- |
| مواضيع طبية MeSH: | Carcinoma, Squamous Cell*/pathology , Mouth Neoplasms*/pathology , Head and Neck Neoplasms*, Humans ; Squamous Cell Carcinoma of Head and Neck ; Cell Line, Tumor ; Cell Proliferation ; Cholesterol ; Epithelial-Mesenchymal Transition/genetics ; Cell Movement |
| مستخلص: | Background: Cholesterol in cell membranes is crucial for cell signaling, adhesion, and migration. Membranes feature cholesterol-rich caveolae with caveolin proteins, playing roles in epithelial-mesenchymal transition and cancer progression. Despite elevated cholesterol levels in tumors, its precise function and the effects of its depletion in oral squamous cell carcinoma remain unclear. The aim of this study was to evaluate the influence of cholesterol depletion in oral squamous cell carcinoma cell line and epithelial-mesenchymal transition process. Methods: Cholesterol depletion was induced on SCC-9 cells by methyl-β-cyclodextrin and cell viability, proliferation, apoptosis, and colony formation capacities were evaluated. Gene and protein expressions were evaluated by reverse transcription polymerase chain reaction (RT-qPCR) and Western Blot, respectively, and cell sublocalization was assessed by immunofluorescence. Results: Cholesterol depletion resulted in alteration of oral squamous cell carcinoma cell morphology at different concentrations of methyl-β-cyclodextrin, as well as decreased cell proliferation and viability rates. Analysis of CAV1 transcript expression revealed increased gene expression in the treated SCC-9 during the 24 h period, at different concentrations of methyl-β-cyclodextrin: 5 , 7.5, 10, and 15 mM, in relation to parental SCC-9. CAV1 protein expression was increased, with subsequent dose-dependent decrease. A statistically significant difference was observed in samples treated with 5 mM of methyl-β-cyclodextrin (p = 0.02, Kruskal-Wallis test). The immunofluorescence assay showed lower cytoplasmic and membrane labeling intensity in the treated samples for CAV1. Conclusion: These findings indicate the modulation of cholesterol as a possible mechanism underlying the regulation of these molecules and activation of epithelial-mesenchymal transition in oral squamous cell carcinoma. (© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.) |
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| معلومات مُعتمدة: | 017/2013 Fundação de Amparo à Pesquisa do Estado da Bahia; MCTI/CNPq-14/2014 Conselho Nacional de Desenvolvimento Científico e Tecnológico |
| فهرسة مساهمة: | Keywords: caveolin; cell adhesion; cholesterol depletion; epithelial–mesenchymal transition; oral squamous cell carcinoma |
| المشرفين على المادة: | 97C5T2UQ7J (Cholesterol) |
| تواريخ الأحداث: | Date Created: 20240320 Date Completed: 20240418 Latest Revision: 20240418 |
| رمز التحديث: | 20240418 |
| DOI: | 10.1111/jop.13524 |
| PMID: | 38503722 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1600-0714 |
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| DOI: | 10.1111/jop.13524 |