دورية أكاديمية
Ocu-miR-10a-5p promotes the chondrogenic differentiation of rabbit BMSCs by targeting BTRC-mediated Wnt/β-catenin signaling pathway.
| العنوان: | Ocu-miR-10a-5p promotes the chondrogenic differentiation of rabbit BMSCs by targeting BTRC-mediated Wnt/β-catenin signaling pathway. |
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| المؤلفون: | Liu D; Department of Spine Surgery, Guangzhou Hospital of Integrated Traditional and Western Medicine, Guangzhou, China., Tang W; Department of Spine Surgery, Guangzhou Hospital of Integrated Traditional and Western Medicine, Guangzhou, China., Tang D; Department of Joint Surgery, Guangzhou Hospital of Integrated Traditional and Western Medicine, 87 Yingbin Road, Huadu District, Guangzhou City, Guangdong Province, China., Yan H; Guangzhou University of Chinese Medicine, Guangzhou, China., Jiao F; Department of Joint Surgery, Guangzhou Hospital of Integrated Traditional and Western Medicine, 87 Yingbin Road, Huadu District, Guangzhou City, Guangdong Province, China. twjiaofeng123@163.com. |
| المصدر: | In vitro cellular & developmental biology. Animal [In Vitro Cell Dev Biol Anim] 2024 Apr; Vol. 60 (4), pp. 343-353. Date of Electronic Publication: 2024 Mar 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Germany NLM ID: 9418515 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1543-706X (Electronic) Linking ISSN: 10712690 NLM ISO Abbreviation: In Vitro Cell Dev Biol Anim Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Berlin : Springer Original Publication: Columbia, MD : Tissue Culture Association, c1993- |
| مواضيع طبية MeSH: | MicroRNAs*/genetics , MicroRNAs*/metabolism , Mesenchymal Stem Cells*/metabolism , Mesenchymal Stem Cells*/cytology , Cell Differentiation*/genetics , Chondrogenesis*/genetics , Wnt Signaling Pathway*/genetics, Animals ; Rabbits ; Chondrocytes/metabolism ; Chondrocytes/cytology ; Ubiquitin-Protein Ligases/metabolism ; Ubiquitin-Protein Ligases/genetics ; Apoptosis/genetics ; Cell Survival ; beta Catenin/metabolism ; beta Catenin/genetics ; Base Sequence ; Gene Expression Regulation |
| مستخلص: | MicroRNAs (miRNAs) play an important role in articular cartilage damage in osteoarthritis (OA). However, the biological role of miRNAs in the chondrogenic differentiation of bone marrow mesenchymal stem cell (BMSC) remains largely unclear. Rabbit bone marrow mesenchymal stem cells (rBMSCs) were isolated, cultured, and identified. Afterwards, rBMSCs were induced to chondrogenic differentiation, examined by Alcian Blue staining. Differentially expressed miRNAs were identified in rBMSCs between induced and non-induced groups by miRNA sequencing analysis, part of which was validated via PCR assay. Cell viability and apoptosis were assessed by CCK-8 assay and Hoechst staining. Saffron O staining was utilized to assess chondrocyte hyperplasia. The expression of specific chondrogenic markers, including COL2A1, SOX9, Runx2, MMP-13, Aggrecan, and BMP-2, were measured at mRNA and protein levels. The association between beta-transducin repeat containing E3 ubiquitin protein ligase (BTRC) and miR-10a-5p in the miRNA family from rabbit (ocu-miR-10a-5p) was determined by luciferase reporter assay. A total of 76 differentially expressed miRNAs, including 52 downregulated and 24 upregulated miRNAs, were identified in rBMSCs from the induced group. Inhibition of ocu-miR-10a-5p suppressed rBMSC viability and chondrogenic differentiation, as well as downregulated the expression of β-catenin, SOX9, COL2A1, MMP-13, and Runx2. BTRC was predicted and confirmed as a target of ocu-miR-10a-5p. Overexpression of BTRC rescued the promoting impacts of overexpressed ocu-miR-10a-5p on chondrogenic differentiation of rBMSCs and β-catenin expression. Taken together, our data suggested that ocu-miR-10a-5p facilitated rabbit BMSC survival and chondrogenic differentiation by activating Wnt/β-catenin signaling through BTRC. (© 2024. The Society for In Vitro Biology.) |
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| معلومات مُعتمدة: | 22-HDWS-069 Guangzhou Huadu District Science and Technology Plan Project |
| فهرسة مساهمة: | Keywords: BTRC; Chondrogenic differentiation; Ocu-miR-10a-5p; Wnt/β-catenin pathway; rBMSCs |
| المشرفين على المادة: | 0 (MicroRNAs) EC 2.3.2.27 (Ubiquitin-Protein Ligases) 0 (beta Catenin) |
| تواريخ الأحداث: | Date Created: 20240320 Date Completed: 20240506 Latest Revision: 20240531 |
| رمز التحديث: | 20240601 |
| DOI: | 10.1007/s11626-024-00888-1 |
| PMID: | 38504085 |
| قاعدة البيانات: | MEDLINE |
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