دورية أكاديمية
أعرض في EDS

Identification and functional analysis of circpdlim5a generated from pdlim5a gene splicing in the skeletal muscle of Japanese flounder (Paralichthys olivaceus).

التفاصيل البيبلوغرافية
العنوان: Identification and functional analysis of circpdlim5a generated from pdlim5a gene splicing in the skeletal muscle of Japanese flounder (Paralichthys olivaceus).
المؤلفون: Wang H; Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266000, China., Liu M; Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266000, China., Tang H; Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266000, China., Zhang Z; Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266000, China., Wen H; Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266000, China., He F; Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266000, China. Electronic address: hefengouc@ouc.edu.cn.
المصدر: General and comparative endocrinology [Gen Comp Endocrinol] 2024 Jun 01; Vol. 352, pp. 114500. Date of Electronic Publication: 2024 Mar 18.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: United States NLM ID: 0370735 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-6840 (Electronic) Linking ISSN: 00166480 NLM ISO Abbreviation: Gen Comp Endocrinol Subsets: MEDLINE
أسماء مطبوعة: Publication: New York, NY : Academic Press
Original Publication: New York.
مواضيع طبية MeSH: Flounder*/metabolism, Animals ; RNA, Circular/genetics ; RNA, Circular/metabolism ; Transcriptome
مستخلص: Circular RNAs (circRNAs) are non-coding RNAs with endogenous regulatory functions, including regulating skeletal muscle development. However, its role in the development of skeletal muscle in Japanese flounder (Paralichthys olivaceus) is not clear. Therefore we screened a candidate circpdlim5a, which is derived from the gene pdlim5a, from the skeletal muscle transcriptome of Japanese flounder. We characterized circpdlim5a, which was more stable compared to the linear RNA pdlim5a. Distributional characterization of circpdlim5a showed that circpdlim5a was predominantly distributed in the nucleus and was highly expressed in the skeletal muscle of adult Japanese flounder (24 months). When we further studied the circpdlim5a function, we found that it inhibited the expression of proliferation and differentiation genes according to the over-expression experiment of circpdlim5a in myoblasts. We concluded that circpdlim5a may inhibit the proliferation and differentiation of myoblasts and thereby inhibit skeletal muscle development in Japanese flounder. This experiment provides information for the study of circRNAs by identifying circpdlim5a and exploring its function, and offers clues for molecular breeding from an epigenetic perspective.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Circpdlim5a; Japanese flounder; Skeletal muscle development
المشرفين على المادة: 0 (RNA, Circular)
تواريخ الأحداث: Date Created: 20240320 Date Completed: 20240422 Latest Revision: 20240607
رمز التحديث: 20240607
DOI: 10.1016/j.ygcen.2024.114500
PMID: 38508470
قاعدة البيانات: MEDLINE
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