دورية أكاديمية
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Sensitive Detection of Ciguatoxins Using a Neuroblastoma Cell-Based Assay with Voltage-Gated Potassium Channel Inhibitors.

التفاصيل البيبلوغرافية
العنوان: Sensitive Detection of Ciguatoxins Using a Neuroblastoma Cell-Based Assay with Voltage-Gated Potassium Channel Inhibitors.
المؤلفون: Yokozeki T; Osaka Saito Laboratory, Japan Food Research Laboratories, 7-4-41 Saitoasagi, Osaka 567-0085, Ibaraki, Japan.; Department of Biological Chemistry, Graduate School of Science, Osaka Metropolitan University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Kanagawa, Japan., Kawabata M; Department of Biological Chemistry, Graduate School of Science, Osaka Metropolitan University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Kanagawa, Japan., Fujita K; Osaka Saito Laboratory, Japan Food Research Laboratories, 7-4-41 Saitoasagi, Osaka 567-0085, Ibaraki, Japan., Hirama M; Department of Biological Chemistry, Graduate School of Science, Osaka Metropolitan University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Kanagawa, Japan., Tsumuraya T; Department of Biological Chemistry, Graduate School of Science, Osaka Metropolitan University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Kanagawa, Japan.
المصدر: Toxins [Toxins (Basel)] 2024 Feb 29; Vol. 16 (3). Date of Electronic Publication: 2024 Feb 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101530765 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6651 (Electronic) Linking ISSN: 20726651 NLM ISO Abbreviation: Toxins (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI
مواضيع طبية MeSH: Ciguatoxins* , Ciguatera Poisoning* , Potassium Channels, Voltage-Gated* , Neuroblastoma*, Humans ; Animals ; Aminopyridines
مستخلص: Ciguatoxins (CTXs) are neurotoxins responsible for ciguatera poisoning (CP), which affects more than 50,000 people worldwide annually. The development of analytical methods to prevent CP is a pressing global issue, and the N2a assay is one of the most promising methods for detecting CTXs. CTXs are highly toxic, and an action level of 0.01 μg CTX1B equivalent (eq)/kg in fish has been proposed. It is desirable to further increase the detection sensitivity of CTXs in the N2a assay to detect such low concentrations reliably. The opening of voltage-gated sodium channels (Na V channels) and blocking of voltage-gated potassium channels (K V channels) are thought to be involved in the toxicity of CTXs. Therefore, in this study, we developed an assay that could detect CTXs with higher sensitivity than conventional N2a assays, using K V channel inhibitors as sensitizing reagents for N2a cells. The addition of the K V channel inhibitors 4-aminopyridine and tetraethylammonium chloride to N2a cells, in addition to the traditional sensitizing reagents ouabain and veratridine, increased the sensitivity of N2a cells to CTXs by up to approximately 4-fold. This is also the first study to demonstrate the influence of K V channels on the toxicity of CTXs in a cell-based assay.
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معلومات مُعتمدة: JP22K05332 Japan Society for the Promotion of Science
فهرسة مساهمة: Keywords: 4-aminopyridine; ciguatoxins; neuroblastoma cell-based assay; tetraethylammonium chloride; voltage-gated potassium channels; voltage-gated sodium channels
المشرفين على المادة: 11050-21-8 (Ciguatoxins)
0 (Aminopyridines)
0 (Potassium Channels, Voltage-Gated)
تواريخ الأحداث: Date Created: 20240327 Date Completed: 20240328 Latest Revision: 20240330
رمز التحديث: 20240330
مُعرف محوري في PubMed: PMC10974581
DOI: 10.3390/toxins16030118
PMID: 38535783
قاعدة البيانات: MEDLINE
الوصف
تدمد:2072-6651
DOI:10.3390/toxins16030118