دورية أكاديمية
Insights on the genomic diversity, virulence and resistance profile of a Campylobacter jejuni strain isolated from a hospitalized patient in Brazil.
| العنوان: | Insights on the genomic diversity, virulence and resistance profile of a Campylobacter jejuni strain isolated from a hospitalized patient in Brazil. |
|---|---|
| المؤلفون: | Gomes CN; Departamento de Análises Clínicas, Toxicológicas E Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto- Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil., Frazão MR; Departamento de Análises Clínicas, Toxicológicas E Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto- Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil., Seribelli AA; Laboratório de Patogenicidade Microbiana E Imunidade Inata, Faculdade de Medicina de Ribeirão Preto- Universidade de São Paulo, São Paulo, Brazil., Barker DOR; National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada., Che EV; National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada., Nogueira MCL; Centro de Investigação de Microrganismos, Departamento de Doenças Dermatológicas, Infecciosas E Parasitárias- Faculdade de Medicina de São José Do Rio Preto, São José Do Rio Preto, São Paulo, Brazil., Taboada EN; National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada., Falcão JP; Departamento de Análises Clínicas, Toxicológicas E Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto- Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil. jufalcao@fcfrp.usp.br. |
| المصدر: | Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2024 Jun; Vol. 55 (2), pp. 1381-1391. Date of Electronic Publication: 2024 Mar 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer International Publishing Country of Publication: Brazil NLM ID: 101095924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1678-4405 (Electronic) Linking ISSN: 15178382 NLM ISO Abbreviation: Braz J Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2019- : Switzerland, AG : Springer International Publishing Original Publication: Rio de Janeiro, RJ, Brasil : Sociedade Brasileira de Microbiologia |
| مواضيع طبية MeSH: | Campylobacter Infections*/microbiology , Anti-Bacterial Agents*/pharmacology , Campylobacter jejuni*/genetics , Campylobacter jejuni*/pathogenicity , Campylobacter jejuni*/drug effects , Campylobacter jejuni*/isolation & purification , Virulence Factors*/genetics, Humans ; Brazil ; Virulence/genetics ; Caco-2 Cells ; Genome, Bacterial ; Drug Resistance, Bacterial ; Genetic Variation ; Microbial Sensitivity Tests ; Multilocus Sequence Typing ; Whole Genome Sequencing |
| مستخلص: | Campylobacteriosis is currently recognized as one of the major causes of foodborne bacterial diseases worldwide. In Brazil, there is insufficient data to estimate the impact of Campylobacter in public health. The aim of this present study was to characterize a C. jejuni CJ-HBSJRP strain isolated from a hospitalized patient in Brazil by its ability to invade human Caco-2 epithelial cells, to survive in U937 human macrophages, and to assess its phenotypic antimicrobial resistance profile. In addition, prophages, virulence and antimicrobial resistance genes were search using whole-genome sequencing data. The genetic relatedness was evaluated by MLST and cgMLST analysis by comparison with 29 other C. jejuni genomes isolated from several countries. The CJ-HBSJRP strain showed an invasion percentage of 50% in Caco-2 polarized cells, 37.5% of survivability in U937 cells and was phenotypically resistant to ampicillin, ciprofloxacin and nalidixic acid. A total of 94 virulence genes related to adherence, biofilm, chemotaxis, immune modulation, invasion process, metabolism, motility and toxin were detected. The resistance genes bla (© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.) |
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| معلومات مُعتمدة: | 2015/23408-0 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2018/26043-1 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2019/19338-8 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2022/07013-0 Fundação de Amparo à Pesquisa do Estado de São Paulo; 304399/2018-3 Conselho Nacional de Desenvolvimento Científico e Tecnológico; 304803/2021-9 Conselho Nacional de Desenvolvimento Científico e Tecnológico |
| فهرسة مساهمة: | Keywords: Antimicrobial resistance profile; Caco-2 and U-937 cell lines; Campylobacter jejuni; Virulence; Whole genome sequence |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Virulence Factors) |
| تواريخ الأحداث: | Date Created: 20240328 Date Completed: 20240605 Latest Revision: 20240608 |
| رمز التحديث: | 20240608 |
| مُعرف محوري في PubMed: | PMC11153483 |
| DOI: | 10.1007/s42770-024-01314-0 |
| PMID: | 38546951 |
| قاعدة البيانات: | MEDLINE |
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