دورية أكاديمية
Expression analysis and biological regulation of silencing regulatory protein 6 (SIRT6) in cutaneous squamous cell carcinoma.
| العنوان: | Expression analysis and biological regulation of silencing regulatory protein 6 (SIRT6) in cutaneous squamous cell carcinoma. |
|---|---|
| المؤلفون: | Chen S; Department of Dermatology, Affiliated Hospital 2 of Nantong University, People's Republic of China., Chen H; Department of Dermatology, Affiliated Hospital 2 of Nantong University, People's Republic of China., Wang X; Department of Dermatology, Affiliated Hospital 2 of Nantong University, People's Republic of China., Zhang D; Jiangsu Provincial Medical Key Discipline (Laboratory) Cultivation Unit of Immunology, Nantong First People's Hospital, People's Republic of China; Medical Research Center, Affiliated Hospital 2 of Nantong University, People's Republic of China., Zhang L; Department of Dermatology, Affiliated Hospital 2 of Nantong University, People's Republic of China., Cheng J; Department of Dermatology, Affiliated Hospital 2 of Nantong University, People's Republic of China., Zhang Q; Department of Dermatology, Affiliated Hospital 2 of Nantong University, People's Republic of China., Hua Z; Department of Dermatology, Affiliated Hospital 2 of Nantong University, People's Republic of China., Miao X; Department of Dermatology, Affiliated Hospital 2 of Nantong University, People's Republic of China., Shi J; Department of Dermatology, Affiliated Hospital 2 of Nantong University, People's Republic of China. Electronic address: jsntmcsj@163.com. |
| المصدر: | Anais brasileiros de dermatologia [An Bras Dermatol] 2024 Jul-Aug; Vol. 99 (4), pp. 535-545. Date of Electronic Publication: 2024 Mar 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier España, S.L Country of Publication: Spain NLM ID: 0067662 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1806-4841 (Electronic) Linking ISSN: 03650596 NLM ISO Abbreviation: An Bras Dermatol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Barcelona, Spain : Elsevier España, S.L. Original Publication: Rio De Janeiro : Sociedade Brasileira de Dermatologia |
| مواضيع طبية MeSH: | Apoptosis* , Carcinoma, Squamous Cell*/pathology , Carcinoma, Squamous Cell*/genetics , Cell Proliferation* , Sirtuins*/genetics , Sirtuins*/metabolism , Skin Neoplasms*/pathology , Skin Neoplasms*/genetics, Aged ; Female ; Humans ; Male ; Middle Aged ; Biomarkers, Tumor/metabolism ; Biomarkers, Tumor/analysis ; Blotting, Western ; Cell Cycle/physiology ; Cell Line, Tumor ; Cell Movement ; Epithelial-Mesenchymal Transition ; Gene Expression Regulation, Neoplastic ; Immunohistochemistry |
| مستخلص: | Background: Cutaneous squamous cell carcinoma (CSCC) is one of the most common types of skin cancer worldwide. Therefore, the identification of biomarkers associated with CSCC progression could aid in the early detection of high-risk squamous cell carcinoma and the development of novel therapeutic strategies. Objective: This study aimed to investigate the expression patterns of silent mating type Information Regulation 2 homolog 6 (SIRT6) in CSCC and its clinical significance. Methods: The protein expression level of SIRT6 in tissues was detected by immunohistochemistry, and the correlation between SIRT6 expression and clinicopathological parameters in CSCC patients was analyzed. The relative expression of SIRT6 in CSCC cell lineage and tissue specimens was determined by western blotting and PCR. The effect of SIRT6 silencing on cell proliferation was evaluated using cell counting kit 8. Wound healing, transwell method, and flow cytometry were used to investigate the migration, invasion, and cell cycle distribution/apoptosis of CSCC cells after SIRT6 silencing, respectively. Western blot was used to detect the expression of EMT (Epithelial-Mesenchymal Transition), cycle, apoptosis, and other related proteins. Results: The high expression of SIRT6 was correlated with the location of cancer tissue and Broder staging in CSCC patients. Knockdown of SIRT6 inhibited the proliferation, migration, invasion and EMT of CSCC cells, and promoted their apoptosis, with cells blocked in G1 phase. Study Limitations: No animal experiments were conducted to further verify the results. Conclusion: Decreased expression of SIRT6 can inhibit the occurrence and development of CSCC. (Copyright © 2024 Sociedade Brasileira de Dermatologia. Published by Elsevier España, S.L.U. All rights reserved.) |
| References: | Cell Stem Cell. 2016 Apr 7;18(4):495-507. (PMID: 27058938) Cell Death Dis. 2018 Jan 19;9(2):56. (PMID: 29352194) Cell. 2009 Jan 9;136(1):62-74. (PMID: 19135889) Int J Dermatol. 2018 Oct;57(10):1208-1217. (PMID: 30105812) Int J Mol Sci. 2018 Jul 12;19(7):. (PMID: 30002310) JAMA Dermatol. 2016 Apr;152(4):419-28. (PMID: 26762219) Acta Derm Venereol. 2016 Aug 23;96(6):820-1. (PMID: 26631391) Hematol Oncol Clin North Am. 2019 Feb;33(1):1-12. (PMID: 30497667) Cancer Res. 2014 Oct 15;74(20):5925-33. (PMID: 25320180) Mol Oncol. 2021 Feb;15(2):697-709. (PMID: 33244865) J Am Acad Dermatol. 2013 Jun;68(6):957-66. (PMID: 23375456) J Cancer Res Clin Oncol. 2020 Dec;146(12):3215-3231. (PMID: 32865618) Trends Biochem Sci. 2014 Feb;39(2):72-81. (PMID: 24438746) Cell Mol Biol Lett. 2020 Mar 05;25:12. (PMID: 32161621) J Clin Oncol. 2018 Sep 1;36(25):2612-2620. (PMID: 30016177) Protein Cell. 2013 Sep;4(9):702-10. (PMID: 23982738) EMBO J. 2013 Aug 14;32(16):2248-63. (PMID: 23860128) Int J Clin Exp Pathol. 2019 Jul 01;12(7):2526-2535. (PMID: 31934079) Blood. 2016 Mar 3;127(9):1138-50. (PMID: 26675349) Oncol Rep. 2017 Aug;38(2):1059-1066. (PMID: 28656307) Nature. 2009 Jul 30;460(7255):587-91. (PMID: 19641587) Anticancer Res. 2018 Oct;38(10):5825-5830. (PMID: 30275206) J Biol Chem. 2012 Nov 30;287(49):40924-37. (PMID: 23086953) Cell. 2012 Dec 7;151(6):1185-99. (PMID: 23217706) Blood. 2015 Jun 11;125(24):3720-30. (PMID: 25931583) Genes Cancer. 2017 Sep;8(9-10):701-712. (PMID: 29234488) Theranostics. 2020 Jun 12;10(16):7465-7479. (PMID: 32642006) BMC Cancer. 2022 Jul 18;22(1):787. (PMID: 35850759) Biomed Pharmacother. 2017 Dec;96:434-442. (PMID: 29031202) Eur J Cancer. 2020 Mar;128:83-102. (PMID: 32113942) Mol Med Rep. 2019 Aug;20(2):1575-1582. (PMID: 31257493) Biochem J. 2007 May 15;404(1):1-13. (PMID: 17447894) |
| فهرسة مساهمة: | Keywords: Biomarkers; Carcinoma, squamous cell; Histology; Sirtuin-6 |
| المشرفين على المادة: | 0 (Biomarkers, Tumor) EC 3.5.1.- (SIRT6 protein, human) EC 3.5.1.- (Sirtuins) |
| تواريخ الأحداث: | Date Created: 20240328 Date Completed: 20240610 Latest Revision: 20240710 |
| رمز التحديث: | 20240710 |
| مُعرف محوري في PubMed: | PMC11220918 |
| DOI: | 10.1016/j.abd.2023.08.010 |
| PMID: | 38548549 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1806-4841 |
|---|---|
| DOI: | 10.1016/j.abd.2023.08.010 |