دورية أكاديمية
أعرض في EDS

A phage nucleus-associated RNA-binding protein is required for jumbo phage infection.

التفاصيل البيبلوغرافية
العنوان: A phage nucleus-associated RNA-binding protein is required for jumbo phage infection.
المؤلفون: Enustun E; Department of Molecular Biology, University of California San Diego, La Jolla, CA 92093, USA., Armbruster EG; Department of Molecular Biology, University of California San Diego, La Jolla, CA 92093, USA., Lee J; Department of Molecular Biology, University of California San Diego, La Jolla, CA 92093, USA., Zhang S; Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA., Yee BA; Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA., Malukhina K; Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA., Gu Y; Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA., Deep A; Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA., Naritomi JT; Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA., Liang Q; Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA., Aigner S; Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA., Adler BA; California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA 94720, USA.; Innovative Genomics Institute, University of California, Berkeley, CA 94720, USA., Cress BF; Innovative Genomics Institute, University of California, Berkeley, CA 94720, USA., Doudna JA; California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA 94720, USA.; Innovative Genomics Institute, University of California, Berkeley, CA 94720, USA.; Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.; Department of Chemistry, University of California, Berkeley, CA 94720, USA.; Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.; MBIB Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA., Chaikeeratisak V; Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand., Cleveland DW; Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA.; Moores Cancer Center, University of California at San Diego, La Jolla, CA, USA., Ghassemian M; Biomolecular and Proteomics Mass Spectrometry Facility, University of California San Diego, La Jolla, CA 92093, USA., Bintu B; Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA., Yeo GW; Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA.; Moores Cancer Center, University of California at San Diego, La Jolla, CA, USA., Pogliano J; Department of Molecular Biology, University of California San Diego, La Jolla, CA 92093, USA., Corbett KD; Department of Molecular Biology, University of California San Diego, La Jolla, CA 92093, USA.; Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA.; Moores Cancer Center, University of California at San Diego, La Jolla, CA, USA.
المصدر: Nucleic acids research [Nucleic Acids Res] 2024 May 08; Vol. 52 (8), pp. 4440-4455.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.
مواضيع طبية MeSH: Bacteriophages*/physiology , RNA-Binding Proteins*/metabolism , RNA-Binding Proteins*/genetics, Cell Nucleus/metabolism ; CRISPR-Cas Systems ; Genome, Viral ; RNA, Messenger/metabolism ; RNA, Messenger/genetics ; RNA, Viral/metabolism ; RNA, Viral/genetics ; Viral Proteins/metabolism ; Viral Proteins/genetics ; Virus Assembly
مستخلص: Large-genome bacteriophages (jumbo phages) of the proposed family Chimalliviridae assemble a nucleus-like compartment bounded by a protein shell that protects the replicating phage genome from host-encoded restriction enzymes and DNA-targeting CRISPR-Cas nucleases. While the nuclear shell provides broad protection against host nucleases, it necessitates transport of mRNA out of the nucleus-like compartment for translation by host ribosomes, and transport of specific proteins into the nucleus-like compartment to support DNA replication and mRNA transcription. Here, we identify a conserved phage nuclear shell-associated protein that we term Chimallin C (ChmC), which adopts a nucleic acid-binding fold, binds RNA with high affinity in vitro, and binds phage mRNAs in infected cells. ChmC also forms phase-separated condensates with RNA in vitro. Targeted knockdown of ChmC using mRNA-targeting dCas13d results in accumulation of phage-encoded mRNAs in the phage nucleus, reduces phage protein production, and compromises virion assembly. Taken together, our data show that the conserved ChmC protein plays crucial roles in the viral life cycle, potentially by facilitating phage mRNA translocation through the nuclear shell to promote protein production and virion development.
(© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.)
التعليقات: Update of: bioRxiv. 2023 Sep 22:2023.09.22.559000. doi: 10.1101/2023.09.22.559000. (PMID: 37790334)
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معلومات مُعتمدة: R35 GM144121 United States GM NIGMS NIH HHS; R01 GM129245 United States NH NIH HHS; S10 OD021724 United States OD NIH HHS; United States HHMI Howard Hughes Medical Institute; R01 GM129245 United States GM NIGMS NIH HHS
المشرفين على المادة: 0 (RNA, Messenger)
0 (RNA, Viral)
0 (RNA-Binding Proteins)
0 (Viral Proteins)
تواريخ الأحداث: Date Created: 20240330 Date Completed: 20240508 Latest Revision: 20240821
رمز التحديث: 20240821
مُعرف محوري في PubMed: PMC11077065
DOI: 10.1093/nar/gkae216
PMID: 38554115
قاعدة البيانات: MEDLINE
الوصف
تدمد:1362-4962
DOI:10.1093/nar/gkae216