دورية أكاديمية
The kinetics of goat sperm is negatively affected after freezing in an extender including zinc oxide nanoparticles.
| العنوان: | The kinetics of goat sperm is negatively affected after freezing in an extender including zinc oxide nanoparticles. |
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| المؤلفون: | Pereira Arruda LC; Andrology Laboratory (ANDROLAB), Veterinary Medicine Departament, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil., de Oliveira Alves Pinto G; Andrology Laboratory (ANDROLAB), Veterinary Medicine Departament, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil. gustavodeoliveiraalvespinto@hotmail.com., Carneiro GF; Andrology Laboratory (ANDROLAB), Veterinary Medicine Departament, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil., Pessoa Guerra MM; Andrology Laboratory (ANDROLAB), Veterinary Medicine Departament, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil. |
| المصدر: | Cryo letters [Cryo Letters] 2024 Mar-Apr; Vol. 45 (2), pp. 100-105. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: CryoLetters Country of Publication: England NLM ID: 9891832 Publication Model: Print Cited Medium: Print ISSN: 0143-2044 (Print) Linking ISSN: 01432044 NLM ISO Abbreviation: Cryo Letters Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London, Eng. : CryoLetters Original Publication: [London : Royal Veterinary College, |
| مواضيع طبية MeSH: | Zinc Oxide*/pharmacology , Semen Preservation*/veterinary, Animals ; Male ; Freezing ; Semen ; Goats ; Cryoprotective Agents/pharmacology ; Cryopreservation/veterinary ; Sperm Motility ; Spermatozoa |
| مستخلص: | Background: Nanotechnology can benefit livestock industries, especially through postharvest semen manipulation. Zinc oxide nanoparticles (Np-ZnO) are potentially an example. Objective: To investigate how the addition of zinc oxide nanoparticles (Np-ZnO) affected the characteristics of post-thawed goat semen. Materials and Methods: Seminal pools from four Saanen bucks were used. Semen was diluted in Tris-egg yolk extender, supplemented with Np-ZnO (0, 50, 100 or 200 ug/mL), frozen and stored in liquid nitrogen (-196 degree C), and thawed in a water bath (37 degree C / 30 s). Semen samples were evaluated for sperm kinetics by computer-assisted sperm analysis (CASA), and assessed for other functional properties by epifluorescence microscopy, such as plasma membrane integrity (PMi), acrosomal membrane integrity (ACi) and mitochondrial membrane potential (MMP). Results: For total motility (TM), the group treated with 200 ug/mL Np-ZnO was superior to the control. In straight-line velocity (VSL), the control was better than the group containing 200 ug/mL of Np-ZnO. For average path velocity (VAP), the control was higher than with 100 ug/mL Np-ZnO. For linearity (LIN), the control was higher than with 200 μg/mL Np-ZnO. In straightness (STR), the control and 100 μg/mL Np-ZnO were higher than with 200 ug/mL Np-ZnO. In wobble (WOB), the control was better than the 50 μg/mL Np-ZnO treatment. In PMi, ACi and MMP no significant differences were found. Conclusion: The addition of Np-ZnO (200 ug/mL) to the goat semen freezing extender improved the total motility of cells, whilst negatively affecting sperm kinetics. https://doi.org/10.54680/fr24210110512. |
| المشرفين على المادة: | SOI2LOH54Z (Zinc Oxide) 0 (Cryoprotective Agents) |
| تواريخ الأحداث: | Date Created: 20240401 Date Completed: 20240403 Latest Revision: 20240403 |
| رمز التحديث: | 20240403 |
| PMID: | 38557988 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0143-2044 |
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