دورية أكاديمية
A novel in vitro model of the small intestinal epithelium in co-culture with 'gut-like' dendritic cells.
| العنوان: | A novel in vitro model of the small intestinal epithelium in co-culture with 'gut-like' dendritic cells. |
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| المؤلفون: | Johnston LJ; Department of Infection Biology, Institute of Infection and Global Health & School of Veterinary Science, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, UK.; Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, UK., Barningham L; Department of Infection Biology, Institute of Infection and Global Health & School of Veterinary Science, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, UK., Campbell EL; Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, UK., Cerovic V; Institute of Molecular Medicine, RWTH University Hospital, Aachen, Germany., Duckworth CA; Institute of Systems, Molecular and Integrative Biology, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, UK., Luu L; Department of Infection Biology, Institute of Infection and Global Health & School of Veterinary Science, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, UK., Wastling J; Department of Infection Biology, Institute of Infection and Global Health & School of Veterinary Science, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, UK.; College of Health, Medicine and Life Sciences, Brunel University London, Kingston Lane, Uxbridge, Middlesex, UK., Derricott H; Department of Infection Biology, Institute of Infection and Global Health & School of Veterinary Science, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, UK., Coombes JL; Department of Infection Biology, Institute of Infection and Global Health & School of Veterinary Science, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, UK.; School of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen, UK. |
| المصدر: | Discovery immunology [Discov Immunol] 2023 Oct 07; Vol. 2 (1), pp. kyad018. Date of Electronic Publication: 2023 Oct 07 (Print Publication: 2023). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press on behalf of the British Society for Immunology Country of Publication: England NLM ID: 9918486782306676 Publication Model: eCollection Cited Medium: Internet ISSN: 2754-2483 (Electronic) Linking ISSN: 27542483 NLM ISO Abbreviation: Discov Immunol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: [Oxford] : Oxford University Press on behalf of the British Society for Immunology, [2022]- |
| مستخلص: | Cross-talk between dendritic cells (DCs) and the intestinal epithelium is important in the decision to mount a protective immune response to a pathogen or to regulate potentially damaging responses to food antigens and the microbiota. Failures in this decision-making process contribute to the development of intestinal inflammation, making the molecular signals that pass between DCs and intestinal epithelial cells potential therapeutic targets. Until now, in vitro models with sufficient complexity to understand these interactions have been lacking. Here, we outline the development of a co-culture model of in vitro differentiated 'gut-like' DCs with small intestinal organoids (enteroids). Sequential exposure of murine bone marrow progenitors to Flt3L, granulocyte macrophage colony-stimulating factor (GM-CSF) and all- trans -retinoic acid (RA) resulted in the generation of a distinct population of conventional DCs expressing CD11b + SIRPα + CD103 +/- (cDC2) exhibiting retinaldehyde dehydrogenase (RALDH) activity. These 'gut-like' DCs extended transepithelial dendrites across the intact epithelium of enteroids. 'Gut-like' DC in co-culture with enteroids can be utilized to define how epithelial cells and cDCs communicate in the intestine under a variety of different physiological conditions, including exposure to different nutrients, natural products, components of the microbiota, or pathogens. Surprisingly, we found that co-culture with enteroids resulted in a loss of RALDH activity in 'gut-like' DCs. Continued provision of GM-CSF and RA during co-culture was required to oppose putative negative signals from the enteroid epithelium. Our data contribute to a growing understanding of how intestinal cDCs assess environmental conditions to ensure appropriate activation of the immune response. Competing Interests: None declared. (© The Author(s) 2023. Published by Oxford University Press on behalf of the British Society for Immunology.) |
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| فهرسة مساهمة: | Keywords: dendritic cell; enteroid; epithelial cell; flow cytometry; mucosal immunology |
| تواريخ الأحداث: | Date Created: 20240403 Latest Revision: 20240404 |
| رمز التحديث: | 20240404 |
| مُعرف محوري في PubMed: | PMC10917230 |
| DOI: | 10.1093/discim/kyad018 |
| PMID: | 38567056 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2754-2483 |
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| DOI: | 10.1093/discim/kyad018 |