دورية أكاديمية
Lactobacillus gasseri BNR17 Ameliorates Dexamethasone-Induced Muscle Loss in BALB/c Mice and C2C12 Myotubes.
| العنوان: | Lactobacillus gasseri BNR17 Ameliorates Dexamethasone-Induced Muscle Loss in BALB/c Mice and C2C12 Myotubes. |
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| المؤلفون: | Lee HY; Department of Microbiology, College of Medicine, Chung-Ang University, Seoul, Korea., Lee J; Department of Microbiology, College of Medicine, Chung-Ang University, Seoul, Korea., Lim H; Department of Microbiology, College of Medicine, Chung-Ang University, Seoul, Korea., Kim HY; Department of Microbiology, College of Medicine, Chung-Ang University, Seoul, Korea., Koo YS; Department of Microbiology, College of Medicine, Chung-Ang University, Seoul, Korea., Lim JS; Department of Microbiology, College of Medicine, Chung-Ang University, Seoul, Korea., Yoon Y; Department of Microbiology, College of Medicine, Chung-Ang University, Seoul, Korea. |
| المصدر: | Journal of medicinal food [J Med Food] 2024 May; Vol. 27 (5), pp. 385-395. Date of Electronic Publication: 2024 Apr 04. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 9812512 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-7600 (Electronic) Linking ISSN: 1096620X NLM ISO Abbreviation: J Med Food Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c1998- |
| مواضيع طبية MeSH: | Dexamethasone*/adverse effects , Mice, Inbred BALB C* , Muscle Fibers, Skeletal*/metabolism , Muscle Fibers, Skeletal*/drug effects , Probiotics* , Muscle Proteins*/metabolism , Muscular Atrophy*/chemically induced , Muscular Atrophy*/metabolism , Muscular Atrophy*/drug therapy , Lactobacillus gasseri*/metabolism , Muscle, Skeletal*/metabolism , Muscle, Skeletal*/drug effects , Ubiquitin-Protein Ligases*/metabolism , Ubiquitin-Protein Ligases*/genetics, Animals ; Mice ; Female ; Male ; SKP Cullin F-Box Protein Ligases/metabolism ; SKP Cullin F-Box Protein Ligases/genetics ; Tripartite Motif Proteins/metabolism ; Tripartite Motif Proteins/genetics ; Forkhead Box Protein O3/metabolism ; Forkhead Box Protein O3/genetics ; Humans ; Insulin-Like Growth Factor I/metabolism ; TOR Serine-Threonine Kinases/metabolism |
| مستخلص: | This study aimed to investigate the effects and mechanism of Lactobacillus gasseri BNR17, a probiotic strain isolated from human breast milk, on dexamethasone-induced muscle loss in mice and cultured myotubes. BALB/c mice were intraperitoneally injected with dexamethasone, and orally administered L. gasseri BNR17 for 21 days. L. gasseri BNR17 treatment ameliorated dexamethasone-induced decline in muscle function, as evidenced by an increase in forelimb grip strength, treadmill running time, and rotarod retention time in both female and male mice. In addition, L. gasseri BNR17 treatment significantly increased the mass of the gastrocnemius and quadriceps muscles. Dual-energy X-ray absorptiometry showed a significant increase in lean body mass and a decrease in fat mass in both whole body and hind limb after treatment with L. gasseri BNR17. It was found that L. gasseri BNR17 treatment downregulated serum myostatin level and the protein degradation pathway composed of muscle-specific ubiquitin E3 ligases, MuRF1 and MAFbx, and their transcription factor FoxO3. In contrast, L. gasseri BNR17 treatment upregulated serum insulin-like growth factor-1 level and Akt-mTOR-p70S6K signaling pathway involved in protein synthesis in muscle. As a result, L. gasseri BNR17 treatment significantly increased the levels of major muscular proteins such as myosin heavy chain and myoblast determination protein 1. Consistent with in vivo results, L. gasseri BNR17 culture supernatant significantly ameliorated dexamethasone-induced C2C12 myotube atrophy in vitro . In conclusion, L. gasseri BNR17 ameliorates muscle loss by downregulating the protein degradation pathway and upregulating the protein synthesis pathway. |
| فهرسة مساهمة: | Keywords: BALB/c; C2C12; Lactobacillus gasseri BNR17; dexamethasone; muscle loss |
| المشرفين على المادة: | 7S5I7G3JQL (Dexamethasone) 0 (Muscle Proteins) EC 2.3.2.27 (Trim63 protein, mouse) EC 2.3.2.27 (Ubiquitin-Protein Ligases) EC 2.3.2.27 (SKP Cullin F-Box Protein Ligases) EC 2.3.2.27 (Fbxo32 protein, mouse) 0 (Tripartite Motif Proteins) 0 (Forkhead Box Protein O3) 0 (FoxO3 protein, mouse) 67763-96-6 (Insulin-Like Growth Factor I) EC 2.7.11.1 (TOR Serine-Threonine Kinases) |
| تواريخ الأحداث: | Date Created: 20240404 Date Completed: 20240530 Latest Revision: 20240530 |
| رمز التحديث: | 20240531 |
| DOI: | 10.1089/jmf.2023.K.0278 |
| PMID: | 38574296 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1557-7600 |
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| DOI: | 10.1089/jmf.2023.K.0278 |