دورية أكاديمية
أعرض في EDS

CytoLyt fixation impedes insulinoma-associated protein 1 (INSM1) immunoreactivity compared to formalin fixation.

التفاصيل البيبلوغرافية
العنوان: CytoLyt fixation impedes insulinoma-associated protein 1 (INSM1) immunoreactivity compared to formalin fixation.
المؤلفون: Garlin-Politis M; Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York Presbyterian Hospital, New York, New York. Electronic address: mg4384@cumc.columbia.edu., Upadhyay Baskota S; Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York Presbyterian Hospital, New York, New York; Department of Pathology and Laboratory Medicine, University of California Davis Health System, Sacramento, California., Picon S; Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York Presbyterian Hospital, New York, New York., Collins N; Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York Presbyterian Hospital, New York, New York., Virk RK; Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York Presbyterian Hospital, New York, New York., Cimic A; Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York Presbyterian Hospital, New York, New York., Yousefi E; Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York Presbyterian Hospital, New York, New York., Gonzalez A; Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York Presbyterian Hospital, New York, New York.
المصدر: Journal of the American Society of Cytopathology [J Am Soc Cytopathol] 2024 May-Jun; Vol. 13 (3), pp. 213-218. Date of Electronic Publication: 2024 Feb 13.
نوع المنشور: Journal Article; Comparative Study
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 101613234 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2213-2945 (Print) Linking ISSN: 22132953 NLM ISO Abbreviation: J Am Soc Cytopathol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York, NY : Elsevier
مواضيع طبية MeSH: Biomarkers, Tumor*/metabolism , Formaldehyde* , Immunohistochemistry*/methods , Repressor Proteins*/immunology , Repressor Proteins*/metabolism , Tissue Fixation*/methods, Female ; Humans ; Male ; Carcinoma, Neuroendocrine/pathology ; Carcinoma, Neuroendocrine/diagnosis ; Carcinoma, Neuroendocrine/metabolism ; Fixatives ; Neuroendocrine Tumors/pathology ; Neuroendocrine Tumors/metabolism ; Neuroendocrine Tumors/diagnosis ; Pancreatic Neoplasms/pathology ; Pancreatic Neoplasms/metabolism ; Pancreatic Neoplasms/diagnosis ; Retrospective Studies
مستخلص: Introduction: Insulinoma-associated protein 1 (INSM1) is an immunohistochemical marker commonly used to confirm cytomorphological concordant neuroendocrine tumors/carcinomas (NETs/NECs), demonstrating high utility in small samples. Previous reports have suggested comparable INSM1 staining in CytoLyt-fixed cell blocks and formalin-fixed surgical pathology specimens. This study aimed to assess INSM1 immunoreactivity using both fixation methods and investigate potential factors contributing to its variable expression.
Materials and Methods: A retrospective query was performed (03/31/21-05/31/22) for NET/NEC cases that had both formalin- and CytoLyt-fixed cell blocks. We collected clinical data and reporting of immunostains for each case. INSM1 staining was evaluated in both fixation methods, and reported as positive, negative, or equivocal. Equivocal INSM1 staining was further scored as a percentage of 1%-100% and intensity of weak (faint staining), moderate (darker staining), and strong (dense staining).
Results: Our search identified 20 cases from diverse body sites, including mediastinal lymph nodes (40%), pancreas (35%), lung (20%), and porta hepatis lymph nodes (5%). All cases exhibited a widespread positivity (over 90%) in formalin-fixed cell blocks. In contrast, CytoLyt fixed cells showed a negative stain in 65% of cases and 30% exhibited an equivocal positivity.
Conclusions: While INSM1 is previously reported as a sensitive (75%-100%) and specific (82.7%-100%) marker for NET/NECs, our study found a reduced immunohistochemical staining in CytoLyt-fixed cell blocks. Consequently, false negative INSM1 immunohistochemical results in CytoLyt-fixed cell block material may pose a pitfall in the diagnosis of NET/NEC.
(Copyright © 2024 American Society of Cytopathology. Published by Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: CytoLyt; Fixation; INSM1; Neuroendocrine; Quality-improvement
المشرفين على المادة: 0 (Biomarkers, Tumor)
0 (Fixatives)
1HG84L3525 (Formaldehyde)
147955-03-1 (INSM1 protein, human)
0 (Repressor Proteins)
تواريخ الأحداث: Date Created: 20240404 Date Completed: 20240511 Latest Revision: 20240628
رمز التحديث: 20240629
DOI: 10.1016/j.jasc.2024.02.002
PMID: 38575468
قاعدة البيانات: MEDLINE
الوصف
تدمد:2213-2945
DOI:10.1016/j.jasc.2024.02.002