دورية أكاديمية
أعرض في EDS

The adult environment promotes the transcriptional maturation of human iPSC-derived muscle grafts.

التفاصيل البيبلوغرافية
العنوان: The adult environment promotes the transcriptional maturation of human iPSC-derived muscle grafts.
المؤلفون: Crist SB; Lillehei Heart Institute, University of Minnesota, Minneapolis, MN, USA.; Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA., Azzag K; Lillehei Heart Institute, University of Minnesota, Minneapolis, MN, USA.; Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA., Kiley J; Lillehei Heart Institute, University of Minnesota, Minneapolis, MN, USA.; Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA., Coleman I; Human Biology Division, Fred Hutchinson Cancer Center, Seattle, WA, USA., Magli A; Lillehei Heart Institute, University of Minnesota, Minneapolis, MN, USA. alemagli@gmail.com.; Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA. alemagli@gmail.com.; Sanofi, Genomic Medicine Unit, 225 2nd Ave, Waltham, MA, 02451, USA. alemagli@gmail.com., Perlingeiro RCR; Lillehei Heart Institute, University of Minnesota, Minneapolis, MN, USA. perli032@umn.edu.; Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA. perli032@umn.edu.
المصدر: NPJ Regenerative medicine [NPJ Regen Med] 2024 Apr 04; Vol. 9 (1), pp. 16. Date of Electronic Publication: 2024 Apr 04.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group, published in partnership with Monash University and Australian Regenerative Medicine Institute Country of Publication: United States NLM ID: 101699846 Publication Model: Electronic Cited Medium: Internet ISSN: 2057-3995 (Electronic) Linking ISSN: 20573995 NLM ISO Abbreviation: NPJ Regen Med Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [New York] : Nature Publishing Group, published in partnership with Monash University and Australian Regenerative Medicine Institute, [2016]-
مستخلص: Pluripotent stem cell (PSC)-based cell therapy is an attractive option for the treatment of multiple human disorders, including muscular dystrophies. While in vitro differentiating PSCs can generate large numbers of human lineage-specific tissue, multiple studies evidenced that these cell populations mostly display embryonic/fetal features. We previously demonstrated that transplantation of PSC-derived myogenic progenitors provides long-term engraftment and functional improvement in several dystrophic mouse models, but it remained unknown whether donor-derived myofibers mature to match adult tissue. Here, we transplanted iPAX7 myogenic progenitors into muscles of non-dystrophic and dystrophic mice and compared the transcriptional landscape of human grafts with respective in vitro-differentiated iPAX7 myotubes as well as human skeletal muscle biospecimens. Pairing bulk RNA sequencing with computational deconvolution of human reads, we were able to pinpoint key myogenic changes that occur during the in vitro-to-in vivo transition, confirm developmental maturity, and consequently evaluate their applicability for cell-based therapies.
(© 2024. The Author(s).)
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معلومات مُعتمدة: R01 AR078571 United States AR NIAMS NIH HHS; R01 AR078624 United States AR NIAMS NIH HHS; R01 AR081882 United States AR NIAMS NIH HHS; T32 HL144472 United States HL NHLBI NIH HHS
تواريخ الأحداث: Date Created: 20240404 Latest Revision: 20240407
رمز التحديث: 20240407
مُعرف محوري في PubMed: PMC10994941
DOI: 10.1038/s41536-024-00360-4
PMID: 38575647
قاعدة البيانات: MEDLINE
الوصف
تدمد:2057-3995
DOI:10.1038/s41536-024-00360-4