دورية أكاديمية
LAMP-visualized photofuel cell self-powered dual-mode sensing platform for detection of transmissible gastroenteritis virus.
| العنوان: | LAMP-visualized photofuel cell self-powered dual-mode sensing platform for detection of transmissible gastroenteritis virus. |
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| المؤلفون: | Yuan R; Key Laboratory of Modern Agricultural Equipment and Technology (Jiangsu University), Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang, 212013, PR China., Ma H; School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, PR China., Min Y; School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, PR China., Ding L; Key Laboratory of Modern Agricultural Equipment and Technology (Jiangsu University), Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang, 212013, PR China., Li B; Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, PR China., Wang K; Key Laboratory of Modern Agricultural Equipment and Technology (Jiangsu University), Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang, 212013, PR China; School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, PR China. Electronic address: wangkun@ujs.edu.cn. |
| المصدر: | Talanta [Talanta] 2024 Jul 01; Vol. 274, pp. 126024. Date of Electronic Publication: 2024 Apr 04. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Oxford : Pergamon Press |
| مواضيع طبية MeSH: | Transmissible gastroenteritis virus*/isolation & purification , Zinc Oxide*/chemistry , Fluorocarbon Polymers* , Nucleic Acid Amplification Techniques* , Molecular Diagnostic Techniques*, Animals ; Swine ; Limit of Detection ; Cadmium Compounds/chemistry ; Electrochemical Techniques/methods ; Electrochemical Techniques/instrumentation ; Nanoparticles/chemistry ; Sulfides/chemistry |
| مستخلص: | The detection of transmissible gastroenteritis virus (TGEV) is of great significance to reduce the loss of pig industry. A LAMP-visualization/PFC self-powered dual-mode output sensor platform was constructed to detect TGEV by combining a simple and intuitive photoelectrochromic material with a highly sensitive PFC self-powered sensing platform without external power supply. The PFC sensing substrate was constructed using CdS nanoparticles modified ZnO NRs (CdS/ZnO NRs) as the photoanode, which exhibited high photoactivity, and Prussian blue (PB) as the cathode. After LAMP reaction on the optical anode, visual signals caused by PB discolorimetry can be detected semi-quantitatively, or PFC power density electrical signals collected by electrochemical workstation can be used. The output power density value is logarithm of TGEV concentration. The linear relationship was good within the detection range of 0.075 fg/μL-7.5 ng/μL, with a detection limit of 0.025 fg/μL (S/N = 3). This multi-signal output sensing platform provides more choices for quantifying TGEV detection results, and the two methods can be mutually verified, which meets the needs of different scenarios and improves the reliability of detection. It has a good effect in the actual sample detection, without the use of expensive and complex instruments, and has a broad application prospect. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: CdS/ZnO NRs; Electrochromic visualization; Loop-mediated isothermal amplification; Photocatalytic fuel cells; Transmissible gastroenteritis virus |
| المشرفين على المادة: | SOI2LOH54Z (Zinc Oxide) 0 (Cadmium Compounds) 39464-59-0 (perfluorosulfonic acid) 0 (Sulfides) 0 (Fluorocarbon Polymers) |
| SCR Protocol: | LAMP assay |
| تواريخ الأحداث: | Date Created: 20240407 Date Completed: 20240503 Latest Revision: 20240503 |
| رمز التحديث: | 20240503 |
| DOI: | 10.1016/j.talanta.2024.126024 |
| PMID: | 38583330 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3573 |
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| DOI: | 10.1016/j.talanta.2024.126024 |