دورية أكاديمية
Green manufacturing of a hypoxanthine enzyme sensor for fish freshness based on modified nitrocellulose surface with chito-oligosaccharide.
| العنوان: | Green manufacturing of a hypoxanthine enzyme sensor for fish freshness based on modified nitrocellulose surface with chito-oligosaccharide. |
|---|---|
| المؤلفون: | Wang Y; Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China., Zhang X; Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China., Wei M; Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China., Kang H; Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China., Zhang Z; Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China., Wang X; Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China; College of Food Science and Engineering, Ocean University of China, Qingdao, 266404, China. Electronic address: xiudan_wang@163.com., Ma C; Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China. Electronic address: mcp169@163.com. |
| المصدر: | Talanta [Talanta] 2024 Jul 01; Vol. 274, pp. 126007. Date of Electronic Publication: 2024 Mar 30. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Oxford : Pergamon Press |
| مواضيع طبية MeSH: | Oligosaccharides*/chemistry , Oligosaccharides*/analysis , Biosensing Techniques*/methods , Hypoxanthine*/analysis , Hypoxanthine*/chemistry , Collodion*/chemistry , Xanthine Oxidase*/chemistry , Xanthine Oxidase*/metabolism, Animals ; Fishes ; Chitin/chemistry ; Enzymes, Immobilized/chemistry ; Enzymes, Immobilized/metabolism ; Green Chemistry Technology/methods ; Surface Properties ; Limit of Detection |
| مستخلص: | Hypoxanthine (Hx), produced by adenosine triphosphate (ATP) metabolism, is a valuable indicator that determines the quality and degradation status of meat products and is also an important biochemical marker to certain diseases such as gout. The rapid emergence of paper-based enzyme biosensors has already revolutionized its on-site determination. But it is still limited by the complex patterning and fabrication, unstable enzyme and uneven coloration. This work aims to develop an eco-friendly method to construct engineered paper microfluidic, which seeks to produce reaction and non-reaction zones without any patterning procedure. Chito-oligosaccharide (COS), derived from shrimp shells, was used to modify nitrocellulose membranes and immobilize xanthine oxidase (XOD) and chromogenic agent of nitro blue tetrazolium chloride (NBT). After modification, micro fluids could converge into the modification area and Hx could be detected by XOD-catalyzed conversion. Due to the positively charged cationic basic properties of COS, the enzyme storage stability and the color homogeneity could be greatly strengthened through the electrostatic attraction between COS and XOD and formazan product. The detection limit (LOD) is 2.30 μM; the linear range is 0.05-0.35 mM; the complete test time can be as short as 5 min. The COS-based biosensor shows high specificity and can be used directly for Hx in complex samples such as fish and shrimp samples, and different broths. This biosensor is eco-friendly, nontechnical, economical and therefore a compelling platform for on-site or home-based detection of food freshness. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Chito-oligosaccharide; Enzyme sensor; Green manufacturing; Hypoxanthine; Paper microfluidics |
| المشرفين على المادة: | 0 (Oligosaccharides) 2TN51YD919 (Hypoxanthine) 9004-70-0 (Collodion) EC 1.17.3.2 (Xanthine Oxidase) 1398-61-4 (Chitin) 0 (Enzymes, Immobilized) |
| تواريخ الأحداث: | Date Created: 20240407 Date Completed: 20240503 Latest Revision: 20240503 |
| رمز التحديث: | 20240503 |
| DOI: | 10.1016/j.talanta.2024.126007 |
| PMID: | 38583331 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3573 |
|---|---|
| DOI: | 10.1016/j.talanta.2024.126007 |