دورية أكاديمية
Role of formyl peptide receptor 2 in steatosis of L02 cells exposed to Mono-(2-ethylhexyl) phthalate.
| العنوان: | Role of formyl peptide receptor 2 in steatosis of L02 cells exposed to Mono-(2-ethylhexyl) phthalate. |
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| المؤلفون: | Feng X; Department of Neurology, the First Hospital of Jilin University, Changchun, China., Zhang R; Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, China., Miao X; Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, China., Li X; Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, China., Cui J; Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, China., Xu H; Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, China., Fang X; Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, China., Zhou C; Department of Neurology, the First Hospital of Jilin University, Changchun, China., Ye L; Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, China., Zhou L; Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, China. |
| المصدر: | Environmental toxicology [Environ Toxicol] 2024 Jul; Vol. 39 (7), pp. 3967-3979. Date of Electronic Publication: 2024 Apr 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley & Sons Country of Publication: United States NLM ID: 100885357 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-7278 (Electronic) Linking ISSN: 15204081 NLM ISO Abbreviation: Environ Toxicol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY : John Wiley & Sons, c1999- |
| مواضيع طبية MeSH: | Diethylhexyl Phthalate*/analogs & derivatives , Diethylhexyl Phthalate*/toxicity , Receptors, Formyl Peptide*/metabolism , Receptors, Lipoxin*/metabolism , Fatty Liver*/chemically induced , Fatty Liver*/pathology , Fatty Liver*/metabolism, Humans ; Cell Line ; Lipid Metabolism/drug effects |
| مستخلص: | Mono-(2-ethylhexyl) phthalate (MEHP) can accumulate in the liver and then lead to hepatic steatosis, while the underlying mechanism remains unclear. Inflammation plays an important role in the disorder of hepatic lipid metabolism. This study aims to clarify the role of the inflammatory response mediated by formyl peptide receptor 2 (FPR2) in steatosis of L02 cells exposed to MEHP. L02 cells were exposed to MEHP of different concentrations and different time. A steatosis model of L02 cells was induced with oleic acid and the cells were exposed to MEHP simultaneously. In addition, L02 cells were incubated with FPR2 antagonist and then exposed to MEHP. Lipid accumulation was determined by oil red O staining and extraction assay. The indicators related to lipid metabolism and inflammatory response were measured with appropriate kits. The relative expression levels of FPR2 and its ligand were determined by Western blot, and the interaction of them was detected by co-immunoprecipitation. As a result, MEHP exposure could promote the occurrence and progression of steatosis and the secretion of chemokines and inflammatory factors in L02 cells. MEHP could also affect the expression and activation of FPR2 and the secretion of FPR2 ligands. In addition, the promotion effect of MEHP on the secretion of total cholesterol and interleukin 1β in L02 cells could be significantly inhibited by the FPR2 antagonist. We concluded that FPR2 might affect the promotion effect of MEHP on steatosis of L02 cells by mediating inflammatory response. (© 2024 Wiley Periodicals LLC.) |
| References: | Erythropel HC, Maric M, Nicell JA, Leask RL, Yargeau V. Leaching of the plasticizer di(2‐ethylhexyl)phthalate (DEHP) from plastic containers and the question of human exposure. Appl Microbiol Biotechnol. 2014;98(24):9967‐9981. Kastner J, Cooper DG, Marić M, Dodd P, Yargeau V. Aqueous leaching of di‐2‐ethylhexyl phthalate and “green” plasticizers from poly(vinyl chloride). Sci Total Environ. 2012;432:357‐364. Wang L, Li J, Zheng J, Liang J, Li R, Gong Z. Source tracing and health risk assessment of phthalate esters in household tap‐water: a case study of the urban area of Quanzhou, Southeast China. Ecotoxicol Environ Saf. 2022;248:114277. Tran HT, Lin C, Lam SS, et al. Biodegradation of high di‐(2‐Ethylhexyl) phthalate (DEHP) concentration by food waste composting and its toxicity assessment using seed germination test. Environ Pollut. 2023;316(Pt 2):120640. Li Y, Huang G, Zhang L, et al. Phthalate esters (PAEs) in soil and vegetables in solar greenhouses irrigated with reclaimed water. Environ Sci Pollut Res Int. 2020;27(18):22658‐22669. Bazarsadueva SV, Taraskin VV, Budaeva OD, et al. First data on PAE levels in surface water in lakes of the eastern coast of Baikal. Int J Environ Res Public Health. 2023;20(2):1173. Ataei Y, Sun Y, Liu W, Ellie SA, Dong H, Ahmad UM. Health effects of exposure to indoor semi‐volatile organic compounds in Chinese building environment: a systematic review. Int J Environ Res Public Health. 2022;20(1):678. Abtahi M, Dobaradaran S, Torabbeigi M, et al. Health risk of phthalates in water environment: occurrence in water resources, bottled water, and tap water, and burden of disease from exposure through drinking water in tehran, Iran. Environ Res. 2019;173:469‐479. Praveena SM, Teh SW, Rajendran RK, et al. Recent updates on phthalate exposure and human health: a special focus on liver toxicity and stem cell regeneration. Environ Sci Pollut Res Int. 2018;25(12):11333‐11342. Kambia K, Dine T, Gressier B, Dupin‐Spriet T, Luyckx M, Brunet C. Evaluation of the direct toxicity of trioctyltrimellitate (TOTM), di(2‐ethylhexyl) phthalate (DEHP) and their hydrolysis products on isolated rat hepatocytes. Int J Artif Organs. 2004;27(11):971‐978. Chen H, Zhang W, Rui BB, Yang SM, Xu WP, Wei W. Di(2‐ethylhexyl) phthalate exacerbates non‐alcoholic fatty liver in rats and its potential mechanisms. Environ Toxicol Pharmacol. 2016;42:38‐44. Zhang Y, Wang S, Zhao T, et al. Mono‐2‐ethylhexyl phthalate (MEHP) promoted lipid accumulation via JAK2/STAT5 and aggravated oxidative stress in BRL‐3A cells. Ecotoxicol Environ Saf. 2019;184:109611. Idilman IS, Ozdeniz I, Karcaaltincaba M. Hepatic steatosis: etiology, patterns, and quantification. Semin Ultrasound CT MR. 2016;37(6):501‐510. Khovidhunkit W, Kim MS, Memon RA, et al. Effects of infection and inflammation on lipid and lipoprotein metabolism: mechanisms and consequences to the host. J Lipid Res. 2004;45(7):1169‐1196. Tacke F. Targeting hepatic macrophages to treat liver diseases. J Hepatol. 2017;66(6):1300‐1312. Chen X, Zhuo S, Zhu T, et al. Fpr2 deficiency alleviates diet‐induced insulin resistance through reducing body weight gain and inhibiting inflammation mediated by macrophage chemotaxis and M1 polarization. Diabetes. 2019;68(6):1130‐1142. Abuduhalike R, Sun J, Zhao L, Mahemuti A. Correlation study of venous thromboembolism with SAA, IL‐1, and TNF‐a levels and gene polymorphisms in Chinese population. J Thorac Dis. 2019;11(12):5527‐5534. Recchiuti A, Krishnamoorthy S, Fredman G, Chiang N, Serhan CN. MicroRNAs in resolution of acute inflammation: identification of novel resolvin D1‐miRNA circuits. FASEB J. 2011;25(2):544‐560. Morris T, Stables M, Hobbs A, et al. Effects of low‐dose aspirin on acute inflammatory responses in humans. J Immunol. 2009;183(3):2089‐2096. Lee C, Kim J, Han J, et al. Formyl peptide receptor 2 determines sex‐specific differences in the progression of nonalcoholic fatty liver disease and steatohepatitis. Nat Commun. 2022;13(1):578. Lee C, Han J, Jung Y. Formyl peptide receptor 2 is an emerging modulator of inflammation in the liver. Exp Mol Med. 2023;55(2):325‐332. Xu Q, Zhao T, Ri H, et al. Di(2‐ethylhexyl) phthalate induced thyroid toxicity via endoplasmic reticulum stress: in vivo and in vitro study. Environ Toxicol. 2022;37(12):2924‐2936. Koch HM, Drexler H, Angerer J. Internal exposure of nursery‐school children and their parents and teachers to di(2‐ethylhexyl)phthalate (DEHP). Int J Hyg Environ Health. 2004;207(1):15‐22. Inoue K, Kawaguchi M, Yamanaka R, et al. Evaluation and analysis of exposure levels of di(2‐ethylhexyl) phthalate from blood bags. Clin Chim Acta. 2005;358(1–2):159‐166. Zhou X, Wang LL, Tang WJ, Tang B. Astragaloside IV inhibits protein tyrosine phosphatase 1B and improves insulin resistance in insulin‐resistant HepG2 cells and triglyceride accumulation in oleic acid (OA)‐treated HepG2 cells. J Ethnopharmacol. 2021;268:113556. Galvão I, De Carvalho RVH, Vago JP, et al. The role of annexin A1 in the modulation of the NLRP3 inflammasome. Immunology. 2020;160(1):78‐89. Lim GEH, Tang A, Ng CH, et al. An observational data meta‐analysis on the differences in prevalence and risk factors between MAFLD vs NAFLD. Clin Gastroenterol Hepatol. 2023;21(3):619‐629.e7. Alves‐Bezerra M, Cohen DE. Triglyceride metabolism in the liver. Compr Physiol. 2017;8(1):1‐8. Zhang S, Hong F, Ma C, Yang S. Hepatic lipid metabolism disorder and atherosclerosis. Endocr Metab Immune Disord Drug Targets. 2022;22(6):590‐600. Wältermann M, Hinz A, Robenek H, et al. Mechanism of lipid‐body formation in prokaryotes: how bacteria fatten up. Mol Microbiol. 2005;55(3):750‐763. Loomba R, Friedman SL, Shulman GI. Mechanisms and disease consequences of nonalcoholic fatty liver disease. Cell. 2021;184(10):2537‐2564. Xu M, Wang Y, Xia R, Wei Y, Wei X. Role of the CCL2‐CCR2 signalling axis in cancer: mechanisms and therapeutic targeting. Cell Prolif. 2021;54(10):e13115. Haller H, Bertram A, Nadrowitz F, Menne J. Monocyte chemoattractant protein‐1 and the kidney. Curr Opin Nephrol Hypertens. 2016;25(1):42‐49. Liu B, Xiang L, Ji J, et al. Sparcl1 promotes nonalcoholic steatohepatitis progression in mice through upregulation of CCL2. J Clin Invest. 2021;131(20):e144801. Schmidt‐Arras D, Rose‐John S. IL‐6 pathway in the liver: from physiopathology to therapy. J Hepatol. 2016;64(6):1403‐1415. Shen Y, Malik SA, Amir M, et al. Decreased hepatocyte autophagy leads to synergistic IL‐1β and TNF mouse liver injury and inflammation. Hepatology. 2020;72(2):595‐608. Zhao X, Wang S, Li X, Liu H, Xu S. Cadmium exposure induces TNF‐α‐mediated necroptosis via FPR2/TGF‐β/NF‐κB pathway in swine myocardium. Toxicology. 2021;453:152733. Horewicz VV, Crestani S, De Sordi R, et al. FPR2/ALX activation reverses LPS‐induced vascular hyporeactivity in aorta and increases survival in a pneumosepsis model. Eur J Pharmacol. 2015;746:267‐273. Cooray SN, Gobbetti T, Montero‐Melendez T, et al. Ligand‐specific conformational change of the G‐protein‐coupled receptor ALX/FPR2 determines proresolving functional responses. Proc Natl Acad Sci USA. 2013;110(45):18232‐18237. Sun Z, Huang W, Zheng Y, et al. Fpr2/CXCL1/2 controls rapid neutrophil infiltration to inhibit Streptococcus agalactiae infection. Front Immunol. 2021;12:786602. Chi G, Feng XX, Ru YX, et al. TLR2/4 ligand‐amplified liver inflammation promotes initiation of autoimmune hepatitis due to sustained IL‐6/IL‐12/IL‐4/IL‐25 expression. Mol Immunol. 2018;99:171‐181. Ye F, Chen C, Qin J, Liu J, Zheng C. Genetic profiling reveals an alarming rate of cross‐contamination among human cell lines used in China. FASEB J. 2015;29(10):4268‐4272. Gronert K, Gewirtz A, Madara JL, Serhan CN. Identification of a human enterocyte lipoxin A4 receptor that is regulated by interleukin (IL)‐13 and interferon gamma and inhibits tumor necrosis factor alpha‐induced IL‐8 release. J Exp Med. 1998;187(8):1285‐1294. |
| معلومات مُعتمدة: | 2022B26 Bethune Program of Jilin University; 20240101286JC Natural Science Foundation of Department of Science and Technology of Jilin Province |
| فهرسة مساهمة: | Keywords: formyl peptide receptor 2; hepatocyte; inflammatory response; mono‐(2‐ethylhexyl) phthalate; steatosis |
| المشرفين على المادة: | C42K0PH13C (Diethylhexyl Phthalate) FU2EWB60RT (mono-(2-ethylhexyl)phthalate) 0 (Receptors, Formyl Peptide) 0 (FPR2 protein, human) 0 (Receptors, Lipoxin) |
| تواريخ الأحداث: | Date Created: 20240410 Date Completed: 20240611 Latest Revision: 20240621 |
| رمز التحديث: | 20240621 |
| DOI: | 10.1002/tox.24256 |
| PMID: | 38598732 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1522-7278 |
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| DOI: | 10.1002/tox.24256 |