دورية أكاديمية
Maximum storage time of refrigerated blood and frozen plasma samples from tambaqui, Colossoma macropomum.
| العنوان: | Maximum storage time of refrigerated blood and frozen plasma samples from tambaqui, Colossoma macropomum. |
|---|---|
| المؤلفون: | Sena MC; Laboratory of in Vitro Fertilization, Institute of Biological Sciences, Federal University of Pará, Belém, PA, Brazil. Electronic address: marcelasena14@gmail.com., Sterzelecki FC; Amazonian Aquaculture Biosystems Laboratory - BIOAQUAM, Federal Rural University of Amazonia, Avenida Presidente Tancredo Neves, N° 2501 Bairro: Terra Firme, 66077-830 Belém, PA, Brazil., Gomes PFF; Amazonian Aquaculture Biosystems Laboratory - BIOAQUAM, Federal Rural University of Amazonia, Avenida Presidente Tancredo Neves, N° 2501 Bairro: Terra Firme, 66077-830 Belém, PA, Brazil., Souza HCA; Amazonian Aquaculture Biosystems Laboratory - BIOAQUAM, Federal Rural University of Amazonia, Avenida Presidente Tancredo Neves, N° 2501 Bairro: Terra Firme, 66077-830 Belém, PA, Brazil., Correia de Melo NFA; Amazonian Aquaculture Biosystems Laboratory - BIOAQUAM, Federal Rural University of Amazonia, Avenida Presidente Tancredo Neves, N° 2501 Bairro: Terra Firme, 66077-830 Belém, PA, Brazil., Natividade J; Amazonian Aquaculture Biosystems Laboratory - BIOAQUAM, Federal Rural University of Amazonia, Avenida Presidente Tancredo Neves, N° 2501 Bairro: Terra Firme, 66077-830 Belém, PA, Brazil., Baldisserotto B; Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, 970105-900 Santa Maria, RS, Brazil., Palheta GDA; Amazonian Aquaculture Biosystems Laboratory - BIOAQUAM, Federal Rural University of Amazonia, Avenida Presidente Tancredo Neves, N° 2501 Bairro: Terra Firme, 66077-830 Belém, PA, Brazil., Santos SDSD; Laboratory of in Vitro Fertilization, Institute of Biological Sciences, Federal University of Pará, Belém, PA, Brazil. |
| المصدر: | Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [Comp Biochem Physiol B Biochem Mol Biol] 2024 Aug-Sep; Vol. 273, pp. 110969. Date of Electronic Publication: 2024 Apr 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Country of Publication: England NLM ID: 9516061 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1107 (Electronic) Linking ISSN: 10964959 NLM ISO Abbreviation: Comp Biochem Physiol B Biochem Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Pergamon Original Publication: Oxford : Tarrytown, NY : Pergamon ; Elsevier, c1994- |
| مواضيع طبية MeSH: | Refrigeration*, Animals ; Time Factors ; Plasma/metabolism ; Plasma/chemistry ; Blood Preservation/methods ; Freezing ; Cryopreservation |
| مستخلص: | Blood analysis is an important tool for monitoring the health status of fish, but the time between collection and analysis can affect the outcome of the analysis. This study sought to determine the maximum time refrigerated blood and frozen plasma samples of the tambaqui, Colossoma macropomum, can be stored without affecting analysis. Samples from 12 fish were obtained, stored under refrigeration at 4 °C and evaluated after 0, 24, 48, 72, and 96 h, while samples from 14 fish were centrifuged, and the resulting plasma was frozen at -20 °C and then evaluated after 0, 8, 12, 16 and 20 weeks. The parameters analyzed were hematocrit (Ht), hemoglobin content (Hb), total erythrocytes (RBC), total (WBC) and differential leukocytes, total thrombocytes (TC), glucose content (Glc), total protein (TP), triglyceride content (TG), total cholesterol (CoT), and the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). For refrigerated whole blood samples, mean corpuscular hemoglobin content (MCHC) showed a transient decline in 24 h, and there were decreases in WBC, TC, Glc and TG that persisted until the 72 h sample point (for Glc and TG) or persisted until the 96 h sample point (for WBC and TC). A decrease in RBC was noted from 48 h on, while ALT was significantly higher in the 96 h sample. Significant decreases in lymphocytes, monocytes, neutrophils and eosinophils were noted from 48 h of storage on, while a significant decline in basophil counts were noted over the last two sampled timepoints. The coefficient of variation was greatest at the 96 h timepoint, indicating increased variability in measured parameters after 4 d of refrigeration. Plasma samples frozen at -20 °C showed a significant variation in ALT after 8 weeks, and increases in TP and TG after 20 weeks. Therefore, it is recommended that refrigerated tambaqui whole blood samples be analyzed within 24 h and frozen tambaqui plasma samples analyzed within 8 weeks. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Blood parameters; Fish; Temperature storage; Tropical fish |
| تواريخ الأحداث: | Date Created: 20240411 Date Completed: 20240602 Latest Revision: 20240602 |
| رمز التحديث: | 20240603 |
| DOI: | 10.1016/j.cbpb.2024.110969 |
| PMID: | 38604560 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder كن أول من يترك تعليقا!