دورية أكاديمية
أعرض في EDS

Raf kinase inhibitor protein expression in smooth muscle tumours of the uterus: a diagnostic marker for leiomyosarcoma?

التفاصيل البيبلوغرافية
العنوان: Raf kinase inhibitor protein expression in smooth muscle tumours of the uterus: a diagnostic marker for leiomyosarcoma?
المؤلفون: Greco S; Department of Experimental and Clinical Medicine, Università Politecnica delle Marche, Ancona, Italy., Pinheiro J; Life and Health Sciences Research Institute (ICVS), Health Sciences School, University of Minho, Braga, Portugal.; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal., Cardoso-Carneiro D; Life and Health Sciences Research Institute (ICVS), Health Sciences School, University of Minho, Braga, Portugal.; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal., Giantomassi F; Department of Biomedical Sciences and Public Health, Università Politecnica delle Marche, Ancona, Italy., Pellegrino P; Department of Experimental and Clinical Medicine, Università Politecnica delle Marche, Ancona, Italy., Scaglione G; Department of Woman and Child Health, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy; Università Cattolica del Sacro Cuore, Rome, Italy., Delli Carpini G; Department of Specialist and Odontostomatological Clinical Sciences, Università Politecnica delle Marche, Ancona, Italy., Ciavattini A; Department of Specialist and Odontostomatological Clinical Sciences, Università Politecnica delle Marche, Ancona, Italy., Zannoni GF; Department of Woman and Child Health, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy; Università Cattolica del Sacro Cuore, Rome, Italy., Goteri G; Department of Biomedical Sciences and Public Health, Università Politecnica delle Marche, Ancona, Italy., Martinho O; Life and Health Sciences Research Institute (ICVS), Health Sciences School, University of Minho, Braga, Portugal.; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal., Ciarmela P; Department of Experimental and Clinical Medicine, Università Politecnica delle Marche, Ancona, Italy.. Electronic address: p.ciarmela@univpm.it.
المصدر: Reproductive biomedicine online [Reprod Biomed Online] 2024 Jun; Vol. 48 (6), pp. 103816. Date of Electronic Publication: 2024 Jan 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101122473 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1472-6491 (Electronic) Linking ISSN: 14726483 NLM ISO Abbreviation: Reprod Biomed Online Subsets: MEDLINE
أسماء مطبوعة: Publication: <2009->: Amsterdam : Elsevier
Original Publication: Cambridge, UK : Reproductive Healthcare Ltd.
مواضيع طبية MeSH: Leiomyosarcoma*/metabolism , Leiomyosarcoma*/pathology , Leiomyosarcoma*/diagnosis , Uterine Neoplasms*/metabolism , Uterine Neoplasms*/pathology , Uterine Neoplasms*/genetics , Phosphatidylethanolamine Binding Protein*/metabolism , Phosphatidylethanolamine Binding Protein*/genetics , Leiomyoma*/metabolism , Leiomyoma*/pathology , Leiomyoma*/diagnosis , Biomarkers, Tumor*/metabolism, Humans ; Female ; Smooth Muscle Tumor/metabolism ; Smooth Muscle Tumor/pathology ; Smooth Muscle Tumor/diagnosis ; Cell Line, Tumor ; Middle Aged ; Cell Movement ; Adult ; Immunohistochemistry
مستخلص: Research Question: What is the expression pattern of Raf kinase inhibitory protein (RKIP) in different subtypes of leiomyoma (usual type, cellular, apoplectic or haemorrhagic leiomyoma, leiomyoma with bizarre nuclei and lipoleiomyoma) and leiomyosarcoma specimens, and what is its biological role in leiomyosarcoma cells?
Design: Leiomyoma and leiomyosarcoma specimens underwent immunohistochemistry staining. Leiomyosarcoma SK-LMS-1 cell line was RKIP knocked down and RKIP overexpressed, and cell viability, wound healing migration and clonogenicity assays were carried out.
Results: A higher immunohistochemical expression of RKIP was observed in bizarre leiomyomas, than in usual-type leiomyomas. Decreased expression was also found in cellular leiomyoma, with generally absent staining in leiomyosarcomas. Upon RKIP expression manipulation in SK-LMS-1 cell line, no major differences were observed in cell viability and migration capacity over time. RKIP knockout, however, resulted in a significant increase in the cell's ability to form colonies (P = 0.011).
Conclusion: RKIP distinct expression pattern among leiomyoma histotype and leiomyosarcoma, and its effect on leiomyosarcoma cells on colony formation, encourages further studies of RKIP in uterine smooth muscle disorders.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
معلومات مُعتمدة: 2021.08171.BD Portuguese National funds, through the Foundation for Science and Technology (FCT).; SFRH/BD/141200/2018 Portuguese National funds, through the Foundation for Science and Technology (FCT)
فهرسة مساهمة: Keywords: Histopathology; Leiomyosarcoma; Markers; RKIP; Uterine fibroid
المشرفين على المادة: 0 (Phosphatidylethanolamine Binding Protein)
0 (Biomarkers, Tumor)
0 (PEBP1 protein, human)
تواريخ الأحداث: Date Created: 20240412 Date Completed: 20240609 Latest Revision: 20240924
رمز التحديث: 20240924
DOI: 10.1016/j.rbmo.2024.103816
PMID: 38608337
قاعدة البيانات: MEDLINE
الوصف
تدمد:1472-6491
DOI:10.1016/j.rbmo.2024.103816