دورية أكاديمية
أعرض في EDS

Deciphering the role of brainstem glycinergic neurons during startle and prepulse inhibition.

التفاصيل البيبلوغرافية
العنوان: Deciphering the role of brainstem glycinergic neurons during startle and prepulse inhibition.
المؤلفون: Huang W; Biology Department, University of Massachusetts Amherst, Life Science Laboratories, 240 Thatcher Road, Amherst, MA, 01002, USA., Cano JC; Department of Biological Sciences, University of Texas at El Paso, 500 West University Avenue, El Paso, TX, 79912, USA., Fénelon K; Biology Department, University of Massachusetts Amherst, Life Science Laboratories, 240 Thatcher Road, Amherst, MA, 01002, USA. Electronic address: kfenelon@umass.edu.
المصدر: Brain research [Brain Res] 2024 Aug 01; Vol. 1836, pp. 148938. Date of Electronic Publication: 2024 Apr 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 0045503 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-6240 (Electronic) Linking ISSN: 00068993 NLM ISO Abbreviation: Brain Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam Elsevier/North-Holland Biomedical Press.
مواضيع طبية MeSH: Prepulse Inhibition*/physiology , Neurons*/physiology , Neurons*/metabolism , Reflex, Startle*/physiology , Brain Stem*/physiology , Brain Stem*/metabolism , Glycine Plasma Membrane Transport Proteins*/metabolism , Glycine*/metabolism, Animals ; Mice ; Male ; Optogenetics ; Mice, Transgenic ; Mice, Inbred C57BL ; Synaptic Transmission/physiology ; Central Amygdaloid Nucleus/physiology ; Central Amygdaloid Nucleus/metabolism
مستخلص: Prepulse inhibition (PPI) of the auditory startle response, a key measure of sensorimotor gating, diminishes with age and is impaired in various neurological conditions. While PPI deficits are often associated with cognitive impairments, their reversal is routinely used in experimental systems for antipsychotic drug screening. Yet, the cellular and circuit-level mechanisms of PPI remain unclear, even under non-pathological conditions. We recently showed that brainstem neurons located in the caudal pontine reticular nucleus (PnC) expressing the glycine transporter type 2 (GlyT2 ± ) receive inputs from the central nucleus of the amygdala (CeA) and contribute to PPI but via an uncharted pathway. Here, using tract-tracing, immunohistochemistry and in vitro optogenetic manipulations coupled to field electrophysiological recordings, we reveal the neuroanatomical distribution of GlyT2 ± PnC neurons and PnC-projecting CeA glutamatergic neurons and we provide mechanistic insights on how these glutamatergic inputs suppress auditory neurotransmission in PnC sections. Additionally, in vivo experiments using GlyT2-Cre mice confirm that optogenetic activation of GlyT2 ± PnC neurons enhances PPI and is sufficient to induce PPI in young mice, emphasizing their role. However, in older mice, PPI decline is not further influenced by inhibiting GlyT2 ± neurons. This study highlights the importance of GlyT2 ± PnC neurons in PPI and underscores their diminished activity in age-related PPI decline.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Aging; Amygdala; Brainstem; Glycine; Prepulse inhibition; Sensorimotor gating
المشرفين على المادة: 0 (Glycine Plasma Membrane Transport Proteins)
TE7660XO1C (Glycine)
0 (Slc6a5 protein, mouse)
تواريخ الأحداث: Date Created: 20240414 Date Completed: 20240602 Latest Revision: 20240602
رمز التحديث: 20240603
DOI: 10.1016/j.brainres.2024.148938
PMID: 38615924
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-6240
DOI:10.1016/j.brainres.2024.148938