دورية أكاديمية
Novel Phosphate Transporter-B PvPTB1;1/1;2 Contribute to Efficient Phosphate Uptake and Arsenic Accumulation in As-Hyperaccumulator Pteris vittata .
| العنوان: | Novel Phosphate Transporter-B PvPTB1;1/1;2 Contribute to Efficient Phosphate Uptake and Arsenic Accumulation in As-Hyperaccumulator Pteris vittata . |
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| المؤلفون: | Sun D; School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China., Zhang X; School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China., Zeng Z; School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China., Feng H; School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China.; Guangdong Provincial Key Lab for Environmental Pollution Control and Remediation Technology, Guangdong Provincial Engineering Research Center for Heavy Metal Contaminated Soil Remediation, Sun Yat-sen University, Guangzhou 510275, China., Yin Z; Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization, Agro-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong, China., Guo N; College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, Jiangsu, China., Tang Y; School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China.; Guangdong Provincial Key Lab for Environmental Pollution Control and Remediation Technology, Guangdong Provincial Engineering Research Center for Heavy Metal Contaminated Soil Remediation, Sun Yat-sen University, Guangzhou 510275, China.; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China., Qiu R; School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China.; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.; College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.; Guangdong Provincial Key Laboratory of Agricultural & Rural Pollution Abatement and Environmental Safety, South China Agricultural University, Guangzhou 510642, China., Ma LQ; Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China., Cao Y; School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China.; Guangdong Provincial Key Lab for Environmental Pollution Control and Remediation Technology, Guangdong Provincial Engineering Research Center for Heavy Metal Contaminated Soil Remediation, Sun Yat-sen University, Guangzhou 510275, China.; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China. |
| المصدر: | Environmental science & technology [Environ Sci Technol] 2024 Apr 30; Vol. 58 (17), pp. 7346-7356. Date of Electronic Publication: 2024 Apr 16. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0213155 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5851 (Electronic) Linking ISSN: 0013936X NLM ISO Abbreviation: Environ Sci Technol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington DC : American Chemical Society Original Publication: Easton, Pa. : American Chemical Society, c1967- |
| مواضيع طبية MeSH: | Pteris*/metabolism , Pteris*/genetics , Arsenic*/metabolism , Phosphates*/metabolism , Phosphate Transport Proteins*/metabolism , Phosphate Transport Proteins*/genetics, Arabidopsis/metabolism ; Arabidopsis/genetics ; Soil Pollutants/metabolism ; Biological Transport |
| مستخلص: | Arsenic (As) contamination in soil poses a potential threat to human health via crop uptake. As-hyperaccumulator Pteris vittata serves as a model plant to study As uptake and associated mechanisms. This study focuses on a novel P/AsV transport system mediated by low-affinity phosphate transporter-B 1 family (PTB1) in P. vittata . Here, we identified two plasma-membrane-localized PTB1 genes, PvPTB1;1/1;2 , in vascular plants for the first time, which were 4.4-40-fold greater in expression in P. vittata than in other Pteris ferns. Functional complementation of a yeast P-uptake mutant and enhanced P accumulation in transgenic Arabidopsis thaliana confirmed their role in P uptake. Moreover, the expression of PvPTB1;1/1;2 facilitated the transport and accumulation of As in both yeast and A. thaliana shoots, demonstrating a comparable AsV uptake capacity. Microdissection-qPCR analysis and single-cell transcriptome analysis collectively suggest that PvPTB1;1/1;2 are specifically expressed in the epidermal cells of P. vittata roots. PTB1 may play a pivotal role in efficient P recycling during phytate secretion and hydrolysis in P. vittata roots. In summary, the dual P transport mechanisms consisting of high-affinity Pht1 and low-affinity PTB1 may have contributed to the efficient P/As uptake in P. vittata , thereby contributing to efficient phytoremediation for As-contaminated soils. |
| فهرسة مساهمة: | Keywords: P/As transporters; dual P uptake system; high-affinity P transporter Pht1; low-affinity P transporters PvPTB1;1/1;2; phytate secretion and hydrolysis; phytoremediation |
| المشرفين على المادة: | N712M78A8G (Arsenic) 0 (Phosphates) 0 (Phosphate Transport Proteins) 0 (Soil Pollutants) |
| تواريخ الأحداث: | Date Created: 20240416 Date Completed: 20240430 Latest Revision: 20240502 |
| رمز التحديث: | 20240502 |
| DOI: | 10.1021/acs.est.3c09335 |
| PMID: | 38624169 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-5851 |
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| DOI: | 10.1021/acs.est.3c09335 |