دورية أكاديمية
A Multiplex Fluorescence of Loop Primer Upon Self-Dequenching Loop-Mediated Isothermal Amplification Assay for the Detection of Epstein-Barr Virus and Human Parvovirus B19 in Clinical Transplant Samples.
| العنوان: | A Multiplex Fluorescence of Loop Primer Upon Self-Dequenching Loop-Mediated Isothermal Amplification Assay for the Detection of Epstein-Barr Virus and Human Parvovirus B19 in Clinical Transplant Samples. |
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| المؤلفون: | Xu Y; Department of Blood Transfusion, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China., Li M; Department of Blood Transfusion, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China., Lin M; Department of Blood Transfusion, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China., Lv Y; Department of Blood Transfusion, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China., Cui D; Department of Blood Transfusion, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China., Wang Y; Key Laboratory of Blood Safety Research of Zhejiang Province, Blood Center of Zhejiang Province, Hangzhou, Zhejiang, China., Xie J; Department of Blood Transfusion, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. |
| المصدر: | Viral immunology [Viral Immunol] 2024 May; Vol. 37 (4), pp. 177-185. Date of Electronic Publication: 2024 Apr 16. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 8801552 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-8976 (Electronic) Linking ISSN: 08828245 NLM ISO Abbreviation: Viral Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY : Mary Ann Liebert, Inc., c1987- |
| مواضيع طبية MeSH: | Herpesvirus 4, Human*/genetics , Herpesvirus 4, Human*/isolation & purification , Nucleic Acid Amplification Techniques*/methods , Parvovirus B19, Human*/genetics , Parvovirus B19, Human*/isolation & purification , Epstein-Barr Virus Infections*/diagnosis , Epstein-Barr Virus Infections*/virology , Sensitivity and Specificity* , Molecular Diagnostic Techniques*/methods, Humans ; Fluorescence ; DNA Primers/genetics ; Transplant Recipients ; Parvoviridae Infections/diagnosis ; Parvoviridae Infections/virology ; DNA, Viral/genetics ; Organ Transplantation |
| مستخلص: | Viral infections are major causes of mortality in solid-organ and hematopoietic stem cell transplant recipients. Epstein-Barr virus (EBV) and Parvovirus B19 (B19V) are among the common viral infections after transplantation and were recommended for increased screening in relevant guidelines. Therefore, the development of rapid, specific, and cost-effective diagnostic methods for EBV and B19V is of paramount importance. We applied Fluorescence of Loop Primer Upon Self-Dequenching Loop-mediated Isothermal Amplification (FLOS-LAMP) for the first time to develop a novel multiplex assay for the detection of EBV and B19V; the fluorophore attached to the probe are self-quenched in unbound state. After binding to the dumbbell-shaped DNA target, the fluorophore is dequenched, resulting in fluorescence development. The novel multiplex FLOS-LAMP assay was optimized by testing various ratios of primer sets. This novel assay, with great specificity, did not cross-react with the common virus. For the detection of EBV and B19V, the limits of detection could reach 969 and 798 copies/μL, respectively, and the assay could be completed within 25 min. Applying this novel assay to detect 200 clinical transplant individuals indicated that the novel assay had high specificity and good sensitivity. We developed multiplex FLOS-LAMP assay for the detection of EBV and B19V, which has the potential to become an important tool for clinical transplant patient screening. |
| فهرسة مساهمة: | Keywords: Epstein–Barr virus; fluorescence self-quenching; human parvovirus B19; loop-mediated isothermal amplification (LAMP); transplantation |
| المشرفين على المادة: | 0 (DNA Primers) 0 (DNA, Viral) |
| SCR Protocol: | LAMP assay |
| تواريخ الأحداث: | Date Created: 20240416 Date Completed: 20240508 Latest Revision: 20240513 |
| رمز التحديث: | 20240513 |
| DOI: | 10.1089/vim.2023.0133 |
| PMID: | 38625025 |
| قاعدة البيانات: | MEDLINE |
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