دورية أكاديمية
Efficient production of High-Purity manno-oligosaccharides from guar gum by citric acid and enzymatic hydrolysis.
| العنوان: | Efficient production of High-Purity manno-oligosaccharides from guar gum by citric acid and enzymatic hydrolysis. |
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| المؤلفون: | Xin D; Bio-Agriculture Institute of Shaanxi, Xi'an 710043, Shaanxi, China., Yin H; Bio-Agriculture Institute of Shaanxi, Xi'an 710043, Shaanxi, China., Ran G; Bio-Agriculture Institute of Shaanxi, Xi'an 710043, Shaanxi, China. Electronic address: ranganqiao@163.com. |
| المصدر: | Bioresource technology [Bioresour Technol] 2024 Jun; Vol. 401, pp. 130719. Date of Electronic Publication: 2024 Apr 18. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991- |
| مواضيع طبية MeSH: | Mannans*/chemistry , Plant Gums*/chemistry , Galactans*/chemistry , Citric Acid*/chemistry , Oligosaccharides*/chemistry , beta-Mannosidase*/metabolism, Hydrolysis ; Saccharomyces cerevisiae/metabolism ; Fermentation |
| مستخلص: | Currently, the production of manno-oligosaccharides (MOS) from guar gum faces challenges of low oligosaccharide enzymatic hydrolysis yield and complicated steps in separation and purification. In this work, a potential strategy to address these issues was explored. By combining citric acid pretreatment (300 mM, 130 °C, 1 h) with β-mannanase hydrolysis, an impressive MOS yield of 61.8 % from guar gum (10 %, w/v) was achieved. The key success lay in the optimizing conditions that completely degraded other galactomannans into monosaccharides, which could be easily removable through Saccharomyces cerevisiae fermentation (without additional nutrients). Following ion exchange chromatography for desalination, and concluding with spray drying, 4.57 g of solid MOS with a purity of 90 % was obtained from 10 g of guar gum. This method offers a streamlined and effective pathway for obtaining high-yield and high-purity MOS from guar gum by combining citric acid pretreatment and enzymatic hydrolysis. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Citric acid pretreatment; Enzymatic hydrolysis; Guar gum; Manno-oligosaccharides; β-mannanase |
| المشرفين على المادة: | 0 (Mannans) 0 (Plant Gums) E89I1637KE (guar gum) 0 (Galactans) 2968PHW8QP (Citric Acid) 0 (Oligosaccharides) EC 3.2.1.25 (beta-Mannosidase) |
| تواريخ الأحداث: | Date Created: 20240420 Date Completed: 20240512 Latest Revision: 20240512 |
| رمز التحديث: | 20240513 |
| DOI: | 10.1016/j.biortech.2024.130719 |
| PMID: | 38642662 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2976 |
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| DOI: | 10.1016/j.biortech.2024.130719 |