دورية أكاديمية
أعرض في EDS

High-level de novo biosynthesis of glycosylated zeaxanthin and astaxanthin in Escherichia coli.

التفاصيل البيبلوغرافية
العنوان: High-level de novo biosynthesis of glycosylated zeaxanthin and astaxanthin in Escherichia coli.
المؤلفون: Chen X; Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (A*STAR), Proteos level 4, Nanos, 138673, Singapore., Lim X; Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (A*STAR), Proteos level 4, Nanos, 138673, Singapore., Bouin A; Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (A*STAR), Proteos level 4, Nanos, 138673, Singapore.; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France., Lautier T; Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (A*STAR), Proteos level 4, Nanos, 138673, Singapore.; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France., Zhang C; Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (A*STAR), Proteos level 4, Nanos, 138673, Singapore. zcqsimon@outlook.com.
المصدر: Bioresources and bioprocessing [Bioresour Bioprocess] 2021 Jul 29; Vol. 8 (1), pp. 67. Date of Electronic Publication: 2021 Jul 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer-Verlag, GmbH Country of Publication: Germany NLM ID: 101665551 Publication Model: Electronic Cited Medium: Internet ISSN: 2197-4365 (Electronic) Linking ISSN: 21974365 NLM ISO Abbreviation: Bioresour Bioprocess Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Heidelberg, Germany : Springer-Verlag, GmbH, [2014]-
مستخلص: Because of wide applications in food, feed, pharmaceutical and cosmetic industries, the carotenoid market is growing rapidly. Most carotenoids are hydrophobic, which limits their bioavailability. Glycosylation is a natural route that substantially increases the water solubility, as well as the bioavailability, photostability and biological activities of carotenoids. Here, we report metabolic engineering efforts (e.g., promoter and RBS engineering, optimization of carbon sources and supplementation of bottleneck genes) to produce glycosylated carotenoids in Escherichia coli. By fine-tuning the carotenoid-biosynthetic genes (crtX, crtZ and crtY), our strain produced up to 47.2 mg/L (~ 11,670 ppm) of zeaxanthin glucosides, ~ 78% of the total carotenoids produced. In another construct with mevalonate, astaxanthin pathway and crtX genes, the strain produced a mixture of carotenoid glucosides including astaxanthin and adonixanthin glucosides with a total yield of 8.1 mg/L (1774 ppm). Our work demonstrated a proof-of-concept study for the microbial biosynthesis of glycosylated carotenoids.
(© 2021. The Author(s).)
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معلومات مُعتمدة: A2084c0064 Agency for Science, Technology and Research; A1984c0040z Agency for Science, Technology and Research
فهرسة مساهمة: Keywords: Astaxanthin; Carotenoids; Glucosyltransferase; Glycosylation; Metabolic engineering; Synthetic biology; UPD-glucose; Zeaxanthin
تواريخ الأحداث: Date Created: 20240423 Latest Revision: 20240425
رمز التحديث: 20240425
مُعرف محوري في PubMed: PMC10992962
DOI: 10.1186/s40643-021-00415-0
PMID: 38650269
قاعدة البيانات: MEDLINE
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