دورية أكاديمية
Tricetin suppresses the cell migration and BMP-6 expression through p38 signaling pathways in human retinal pigment epithelium cells.
| العنوان: | Tricetin suppresses the cell migration and BMP-6 expression through p38 signaling pathways in human retinal pigment epithelium cells. |
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| المؤلفون: | Chien HW; Department of Ophthalmology, Cathay General Hospital, Taipei, Taiwan.; Department of Ophthalmology, Sijhih Cathay General Hospital, New Taipei City, Taiwan.; School of Medicine, National Tsing Hua University, Hsinchu, Taiwan.; School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan., Chuang CC; Department of Ophthalmology, Changhua Christian Hospital, Changhua, Taiwan.; Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.; Department of Post-Baccalaureate Medicine, College of Medicine, National Chung Hsing University, Taichung, Taiwan., Hsieh YH; Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.; Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan., Lee CY; Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.; Nobel Eye Institute, Taipei, Taiwan., Yu NY; Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.; Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan., Yang SF; Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.; Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan. |
| المصدر: | Environmental toxicology [Environ Toxicol] 2024 Aug; Vol. 39 (8), pp. 4148-4155. Date of Electronic Publication: 2024 Apr 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley & Sons Country of Publication: United States NLM ID: 100885357 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-7278 (Electronic) Linking ISSN: 15204081 NLM ISO Abbreviation: Environ Toxicol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY : John Wiley & Sons, c1999- |
| مواضيع طبية MeSH: | Retinal Pigment Epithelium*/drug effects , Retinal Pigment Epithelium*/metabolism , Retinal Pigment Epithelium*/cytology , Cell Movement*/drug effects , Bone Morphogenetic Protein 6*/metabolism , p38 Mitogen-Activated Protein Kinases*/metabolism, Humans ; Cell Line ; MAP Kinase Signaling System/drug effects ; Signal Transduction/drug effects |
| مستخلص: | Proliferative vitreoretinopathy (PVR) is a visual-threatening disease, which cause from the migration of retinal pigment epithelium (RPE). Tricetin, a family of flavonoids, can inhibit the metastasis of several cancers. Herein, we aim to evaluate the possible effect of tricetin on inhibiting ARPE-19 cells migration. The Boyden chamber assay, wound healing assay, RNA sequencing, and Western blot analysis were applied in our experiment. The results revealed that tricetin inhibited the cell migration abilities of ARPE-19 cells. Moreover, using RNA sequencing technology, we revealed that tricetin repressed bone morphogenetic protein-6 (BMP-6) gene expressions in ARPE-19 cells. Overexpression of BMP-6 resulted in significant restoration of cell migration capabilities of tricetin-treated ARPE-19 cells. Furthermore, tricetin suppressed the phosphorylation of the p38 signaling pathway. Moreover, blocking the p38 pathway also inhibits BMP-6 expression and migration in the ARPE-19 cells. In conclusion, this study revealed that tricetin inhibits the ARPE-19 cell migration mainly via the suppression of BMP-6 expression and p38 signaling pathway. (© 2024 Wiley Periodicals LLC.) |
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| معلومات مُعتمدة: | CSH-2023-D-010 Chung Shan Medical University Hospital |
| فهرسة مساهمة: | Keywords: BMP‐6; p38; proliferative vitreoretinopathy; retinal pigment epithelium; tricetin |
| المشرفين على المادة: | 0 (Bone Morphogenetic Protein 6) 0 (BMP6 protein, human) EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases) |
| تواريخ الأحداث: | Date Created: 20240424 Date Completed: 20240712 Latest Revision: 20240808 |
| رمز التحديث: | 20240808 |
| DOI: | 10.1002/tox.24294 |
| PMID: | 38654487 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1522-7278 |
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| DOI: | 10.1002/tox.24294 |